دورية أكاديمية
The nature of chronic rejection after lung transplantation: a murine orthotopic lung transplant study.
| العنوان: | The nature of chronic rejection after lung transplantation: a murine orthotopic lung transplant study. |
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| المؤلفون: | Heigl T; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Kaes J; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Aelbrecht C; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Serré J; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Yamada Y; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium.; Department of Thoracic Surgery, Kyoto University Hospital, Kyoto, Japan., Geudens V; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Van Herck A; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Vanstapel A; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium.; Translational Cell and Tissue Research, KU Leuven and UZ Gasthuisberg, Leuven, Belgium., Sacreas A; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Ordies S; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Frick A; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Saez Gimenez B; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium.; Pulmonology Service, Lung Transplant Program, Hospital Universitari Vall d'Hebrón, Barcelona, Spain., Van Slambrouck J; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Beeckmans H; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Acet Oztürk NA; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium.; Department of Respiratory Medicine, Uludag University Faculty of Medicine, Bursa, Türkiye., Orlitova M; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Vaneylen A; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Claes S; Translational Cell and Tissue Research, KU Leuven and UZ Gasthuisberg, Leuven, Belgium., Schols D; Translational Cell and Tissue Research, KU Leuven and UZ Gasthuisberg, Leuven, Belgium., Vande Velde G; Department of Imaging and Pathology, Biomedical MRI/MoSAIC, KU Leuven, Leuven, Belgium., Schupp J; Pulmonary, Critical Care and Sleep Medicine, Yale University School of Medicine, New Haven, CT, United States.; Department of Respiratory Medicine, Hannover Medical School and Biomedical Research in End-stage and Obstructive Lung Disease Hannover, German Lung Research Center (DZL), Hannover, Germany., Kaminski N; Pulmonary, Critical Care and Sleep Medicine, Yale University School of Medicine, New Haven, CT, United States., Boesch M; Laboratory of Hepatology, CHROMETA Department, KU Leuven, Leuven, Belgium., Korf H; Laboratory of Hepatology, CHROMETA Department, KU Leuven, Leuven, Belgium., van der Merwe S; Laboratory of Hepatology, CHROMETA Department, KU Leuven, Leuven, Belgium.; Department of Gastroenterology and Hepatology, UZ Leuven, Leuven, Belgium., Dupont L; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Vanoirbeek J; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Godinas L; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Van Raemdonck DE; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Janssens W; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Gayan-Ramirez G; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Ceulemans LJ; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., McDonough JE; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium.; Pulmonary, Critical Care and Sleep Medicine, Yale University School of Medicine, New Haven, CT, United States., Verbeken EK; Translational Cell and Tissue Research, KU Leuven and UZ Gasthuisberg, Leuven, Belgium., Vos R; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium., Vanaudenaerde BM; Laboratory of Respiratory Diseases and Thoracic Surgery, KULeuven and UZ Gasthuisberg, Leuven, Belgium. |
| المصدر: | Frontiers in immunology [Front Immunol] 2024 Apr 25; Vol. 15, pp. 1369536. Date of Electronic Publication: 2024 Apr 25 (Print Publication: 2024). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Lung Transplantation*/adverse effects , Graft Rejection*/immunology, Animals ; Mice ; Chronic Disease ; Disease Models, Animal ; Mice, Inbred C57BL ; Lung/pathology ; Lung/immunology ; Male ; Bronchiolitis Obliterans/etiology ; Bronchiolitis Obliterans/immunology ; Bronchiolitis Obliterans/pathology |
| مستخلص: | Introduction: Chronic rejection is a major complication post-transplantation. Within lung transplantation, chronic rejection was considered as airway centred. Chronic Lung Allograft Dysfunction (CLAD), defined to cover all late chronic complications, makes it more difficult to understand chronic rejection from an immunological perspective. This study investigated the true nature, timing and location of chronic rejection as a whole, within mouse lung transplantation. Methods: 40 mice underwent an orthotopic left lung transplantation, were sacrificed at day 70 and evaluated by histology and in vivo µCT. For timing and location of rejection, extra grafts were sacrificed at day 7, 35, 56 and investigated by ex vivo µCT or single cell RNA (scRNA) profiling. Results: Chronic rejection originated as innate inflammation around small arteries evolving toward adaptive organization with subsequent end-arterial fibrosis and obliterans. Subsequently, venous and pleural infiltration appeared, followed by airway related bronchiolar folding and rarely bronchiolitis obliterans was observed. Ex vivo µCT and scRNA profiling validated the time, location and sequence of events with endothelial destruction and activation as primary onset. Conclusion: Against the current belief, chronic rejection in lung transplantation may start as an arterial response, followed by responses in venules, pleura, and, only in the late stage, bronchioles, as may be seen in some but not all patients with CLAD. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Heigl, Kaes, Aelbrecht, Serré, Yamada, Geudens, Van Herck, Vanstapel, Sacreas, Ordies, Frick, Saez Gimenez, Van Slambrouck, Beeckmans, Acet Oztürk, Orlitova, Vaneylen, Claes, Schols, Vande Velde, Schupp, Kaminski, Boesch, Korf, van der Merwe, Dupont, Vanoirbeek, Godinas, Van Raemdonck, Janssens, Gayan-Ramirez, Ceulemans, McDonough, Verbeken, Vos and Vanaudenaerde.) |
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| فهرسة مساهمة: | Keywords: chronic rejection; imaging; lung transplantation; mouse model; single-cell profiling |
| تواريخ الأحداث: | Date Created: 20240513 Date Completed: 20240513 Latest Revision: 20240514 |
| رمز التحديث: | 20240514 |
| مُعرف محوري في PubMed: | PMC11084670 |
| DOI: | 10.3389/fimmu.2024.1369536 |
| PMID: | 38736881 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2024.1369536 |