دورية أكاديمية
أعرض في EDS

Novel cereal bran based low-cost liquid medium for enhanced growth, multifunctional traits and shelf life of consortium biofertilizer containing Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.

التفاصيل البيبلوغرافية
العنوان: Novel cereal bran based low-cost liquid medium for enhanced growth, multifunctional traits and shelf life of consortium biofertilizer containing Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.
المؤلفون: Akashdeep; Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab 141004, India., Kumari S; Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab 141004, India. Electronic address: sumanverma@pau.edu., Rani N; School of Organic Farming, Punjab Agricultural University, Ludhiana, Punjab 141004, India.
المصدر: Journal of microbiological methods [J Microbiol Methods] 2024 Jul; Vol. 222, pp. 106952. Date of Electronic Publication: 2024 May 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-
مواضيع طبية MeSH: Bacillus subtilis*/growth & development , Pseudomonas*/growth & development , Azotobacter*/growth & development , Fertilizers* , Dietary Fiber* , Culture Media*/chemistry, Oryza/growth & development ; Oryza/microbiology ; Edible Grain/microbiology ; Edible Grain/growth & development ; Microbial Viability
مستخلص: The present study was carried out to valorise cereal (rice and wheat) bran for the development of low-cost liquid consortium bioformulation. Different concentrations of bran-based liquid media formulations were evaluated for the growth of consortium biofertilizer cultures (Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.). Among the bran-based formulations, wheat bran-based formulation WB5, exhibited the highest viable cell of 10.68 ± 0.09 Log 10 CFU/ml and 12.63 ± 0.04 Log 10 CFU/ml for Azotobacter chroococcum and Bacillus subtilis whereas for Pseudomonas sp., rice bran based bioformulation RB5 recorded maximum viability (12.71 ± 0.05 Log 10 CFU/ml) after 72 h of incubation. RB5 1 and WB5 2 liquid formulations were further optimized for enhanced shelf life using 5, 10 and 15 mM of trehalose, 0.05 and 0.1% carboxymethyl cellulose, and 0.5 and 1.0% glycerol. Following the peak growth at 72 h of incubation, a gradual decrease in the viable population of consortium biofertilizer cultures was observed in all the liquid formulations. The WB5 and RB5 formulations with 15 mM trehalose and 0.1% CMC, not only recorded significantly highest cell count of consortium biofertilizer cultures, but also maximally supported multi-functional traits i.e., phosphate and zinc solubilization, ammonia and IAA production up to 150 days. Further evaluation of seedling emergence and growth of wheat (PBW 826) under axenic conditions recorded WB5 amended with 15 mM trehalose-based consortium bioformulation to exhibit maximum emergence and growth of wheat seedlings. This low-cost liquid formulation can be used for large-scale biofertilizer production as a cost-effective liquid biofertilizer production technology.
Competing Interests: Declaration of competing interest Both the authors declare that they have no conflict of interest and have no relevant financial or non-financial interest to disclose. The current research was supported by the Department of Microbiology and School of Oragic Farming, Punjab Agricultural University, Ludhiana, Punjab, India.
(Copyright © 2024. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: And wheat bran; Consortium biofertilizer; Low-cost bioformulation; Rice
المشرفين على المادة: 0 (Fertilizers)
0 (Dietary Fiber)
0 (Culture Media)
تواريخ الأحداث: Date Created: 20240513 Date Completed: 20240614 Latest Revision: 20240614
رمز التحديث: 20240615
DOI: 10.1016/j.mimet.2024.106952
PMID: 38740286
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-8359
DOI:10.1016/j.mimet.2024.106952