دورية أكاديمية
Delay in the diagnosis of Brucella abortus bacteremia in a nonendemic country: a case report.
| العنوان: | Delay in the diagnosis of Brucella abortus bacteremia in a nonendemic country: a case report. |
|---|---|
| المؤلفون: | Park JH; Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Republic of Korea., Kim TS; Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Republic of Korea.; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea., Park H; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.; Department of Laboratory Medicine, Seoul National University Boramae Medical Center, Seoul, Republic of Korea., Kang CK; Department of Internal Medicine, Seoul National University College of Medicine, 101 Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea. voyager0@snu.ac.kr. |
| المصدر: | BMC infectious diseases [BMC Infect Dis] 2024 May 13; Vol. 24 (1), pp. 489. Date of Electronic Publication: 2024 May 13. |
| نوع المنشور: | Case Reports; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2001- |
| مواضيع طبية MeSH: | Brucellosis*/diagnosis , Brucellosis*/microbiology , Brucellosis*/drug therapy , Brucella abortus*/isolation & purification , Brucella abortus*/genetics , Bacteremia*/diagnosis , Bacteremia*/microbiology , Bacteremia*/drug therapy, Aged ; Humans ; Male ; Delayed Diagnosis ; Anti-Bacterial Agents/therapeutic use ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Animals |
| مستخلص: | Background: It is challenging to diagnose brucellosis in nonendemic regions because it is a nonspecific febrile disease. The accurate identification of Brucella spp. in clinical microbiology laboratories (CMLs) continues to pose difficulties. Most reports of misidentification are for B. melitensis, and we report a rare case of misidentified B. abortus. Case Presentation: A 67-year-old man visited an outpatient clinic complaining of fatigue, fever, and weight loss. The patient had a history of slaughtering cows with brucellosis one year prior, and his Brucella antibody tests were negative twice. After blood culture, the administration of doxycycline and rifampin was initiated. The patient was hospitalized due to a positive blood culture. Gram-negative coccobacilli were detected in aerobic blood culture bottles, but the CML's lack of experience with Brucella prevented appropriate further testing. Inaccurate identification results were obtained for a GN ID card of VITEK 2 (bioMérieux, USA) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using a MALDI Biotyper (Bruker, Germany). The strain showed 100.0% identity with Brucella spp. according to 16S rRNA sequencing. MALDI-TOF MS peaks were reanalyzed using the CDC MicrobeNet database to determine Brucella spp. (score value: 2.023). The patient was discharged after nine days of hospitalization and improved after maintaining only doxycycline for six weeks. The isolate was also identified as Brucella abortus by genomic evidence. Conclusion: Automated identification instruments and MALDI-TOF MS are widely used to identify bacteria in CMLs, but there are limitations in accurately identifying Brucella spp. It is important for CMLs to be aware of the possibility of brucellosis through communication with clinicians. Performing an analysis with an additional well-curated MALDI-TOF MS database such as Bruker security-relevant (SR) database or CDC MicrobeNet database is helpful for quickly identifying the genus Brucella. (© 2024. The Author(s).) |
| References: | Rev Esp Quimioter. 2020 Jun;33(3):223-224. (PMID: 32312029) J Clin Microbiol. 2020 Jan 28;58(2):. (PMID: 31694974) Front Microbiol. 2020 Apr 07;11:468. (PMID: 32373076) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) Mol Biol Evol. 2021 Sep 27;38(10):4647-4654. (PMID: 34320186) Infect Dis (Lond). 2020 Jun;52(6):437-439. (PMID: 32085681) J Clin Microbiol. 2023 Aug 23;61(8):e0043823. (PMID: 37395662) IDCases. 2018 Feb 01;11:74-76. (PMID: 29619327) J Clin Microbiol. 2011 Mar;49(3):1165-8. (PMID: 21209167) FEMS Microbiol Rev. 2021 Jan 8;45(1):. (PMID: 33016322) J Clin Microbiol. 2017 Dec;55(12):3513-3529. (PMID: 29021156) Infect Chemother. 2018 Jun;50(2):149-152. (PMID: 29968983) Bioinformatics. 2018 Jul 1;34(13):i142-i150. (PMID: 29949969) Open Forum Infect Dis. 2021 Oct 01;8(10):ofab473. (PMID: 34660837) Int J Syst Evol Microbiol. 2017 May;67(5):1613-1617. (PMID: 28005526) J Clin Microbiol. 2012 Jun;50(6):2173-5. (PMID: 22495564) Acta Trop. 2023 Dec;248:107036. (PMID: 37793493) J Clin Microbiol. 2016 Mar;54(3):816. (PMID: 26763958) Yonsei Med J. 2017 Jan;58(1):259-260. (PMID: 27873523) Pathogens. 2022 Mar 21;11(3):. (PMID: 35335701) Ann Lab Med. 2019 Nov;39(6):530-536. (PMID: 31240880) Enferm Infecc Microbiol Clin (Engl Ed). 2022 Apr;40(4):210-212. (PMID: 35473994) J Med Microbiol. 2003 May;52(Pt 5):441-442. (PMID: 12721322) J Clin Microbiol. 2018 May 25;56(6):. (PMID: 29802238) PLoS Comput Biol. 2017 Jun 8;13(6):e1005595. (PMID: 28594827) J Clin Microbiol. 2022 Aug 17;60(8):e0031122. (PMID: 35852343) J Clin Microbiol. 2018 Aug 27;56(9):. (PMID: 29925641) Ann Lab Med. 2016 Jul;36(4):390-2. (PMID: 27139618) J Infect Dev Ctries. 2016 Apr 28;10(4):432-6. (PMID: 27131010) Int J Syst Evol Microbiol. 2020 Jul;70(7):4043-4049. (PMID: 32731908) PLoS Negl Trop Dis. 2018 Oct 18;12(10):e0006874. (PMID: 30335748) |
| فهرسة مساهمة: | Keywords: Brucella abortus; Bacteremia; Brucellosis; MALDI–TOF MS; Whole-genome sequencing |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20240513 Date Completed: 20240514 Latest Revision: 20240702 |
| رمز التحديث: | 20240702 |
| مُعرف محوري في PubMed: | PMC11089730 |
| DOI: | 10.1186/s12879-024-09377-y |
| PMID: | 38741035 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1471-2334 |
|---|---|
| DOI: | 10.1186/s12879-024-09377-y |