دورية أكاديمية
Central carbon metabolism exhibits unique characteristics during the handling of fungal patterns by monocyte-derived dendritic cells.
العنوان: | Central carbon metabolism exhibits unique characteristics during the handling of fungal patterns by monocyte-derived dendritic cells. |
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المؤلفون: | Alvarez Y; Departamento de Bioquímica, Biología Molecular y Fisiología, Universidad de Valladolid, 47003, Valladolid, Spain., Mancebo C; Departamento de Bioquímica, Biología Molecular y Fisiología, Universidad de Valladolid, 47003, Valladolid, Spain., Alonso S; Unidad de Excelencia Instituto de Biomedicina y Genética Molecular, CSIC-Universidad de Valladolid, 47003, Valladolid, Spain., Montero O; Unidad de Excelencia Instituto de Biomedicina y Genética Molecular, CSIC-Universidad de Valladolid, 47003, Valladolid, Spain., Fernández N; Departamento de Bioquímica, Biología Molecular y Fisiología, Universidad de Valladolid, 47003, Valladolid, Spain; Unidad de Excelencia Instituto de Biomedicina y Genética Molecular, CSIC-Universidad de Valladolid, 47003, Valladolid, Spain., Sánchez Crespo M; Unidad de Excelencia Instituto de Biomedicina y Genética Molecular, CSIC-Universidad de Valladolid, 47003, Valladolid, Spain. Electronic address: sanchezcrespomariano@gmail.com. |
المصدر: | Redox biology [Redox Biol] 2024 Jul; Vol. 73, pp. 103187. Date of Electronic Publication: 2024 May 09. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier, B.V Country of Publication: Netherlands NLM ID: 101605639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-2317 (Electronic) Linking ISSN: 22132317 NLM ISO Abbreviation: Redox Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Amsterdam]: Elsevier, B.V., [2013]- |
مواضيع طبية MeSH: | Carbon*/metabolism , Dendritic Cells*/metabolism, Humans ; Zymosan/metabolism ; Monocytes/metabolism ; Pentose Phosphate Pathway ; Glycolysis ; Reactive Oxygen Species/metabolism ; Energy Metabolism ; Saccharomyces cerevisiae/metabolism ; Citric Acid Cycle ; NADPH Oxidases/metabolism ; Phagocytosis ; Cytokines/metabolism |
مستخلص: | Monocyte-derived dendritic cells (MDDCs) are key players in the defense against fungal infection because of their outstanding capacity for non-opsonic phagocytosis and phenotypic plasticity. Accordingly, MDDCs rewire metabolism to meet the energetic demands for microbial killing and biomass synthesis required to restore homeostasis. It has been commonplace considering the metabolic reprogramming a mimicry of the Warburg effect observed in tumor cells. However, this may be an oversimplification since the offshoots of glycolysis and the tricarboxylic acid (TCA) cycle are connected in central carbon metabolism. Zymosan, the external wall of Saccharomyces cerevisiae, contains β-glucan and α-mannan chains that engage the C-type lectin receptors dectin-1/2 and Toll-like receptors. This makes it an optimal fungal surrogate for experimental research. Using real-time bioenergetic assays and [U- 13 C]glucose labeling, central hubs connected to cytokine expression were identified. The pentose phosphate pathway (PPP) exhibited a more relevant capacity to yield ribose-5-phosphate than reducing equivalents of NADPH, as judged from the high levels of isotopologues showing 13 C-labeling in the ribose moiety and the limited contribution of the oxidative arm of the PPP to the production of ROS by NADPH oxidases (NOX). The finding of 13 C-label in the purine ring and in glutathione unveiled the contribution of serine-derived glycine to purine ring and glutathione synthesis. Serine synthesis also supported the TCA cycle. Zymosan exhausted NAD + and ATP, consistent with intracellular consumption and/or extracellular export. Poly-ADP-ribosylated proteins detected in the nuclear fractions of MDDCs did not show major changes upon zymosan stimulation, which suggests its dependence on constitutive Fe(II)/2-oxoglutarate-dependent demethylation of 5-methylcytosine by TET translocases and/or demethylation of histone H3 lysine 27 by JMJD demethylases rather than on NOX activities. These results disclose a unique pattern of central carbon metabolism following fungal challenge, characterized by the leverage of glycolysis offshoots and an extensive recycling of NAD + and poly(ADP-ribose). Competing Interests: Declaration of competing interest The authors declare that there are not conflicts of interest. (Copyright © 2024. Published by Elsevier B.V.) |
فهرسة مساهمة: | Keywords: Cytokines; Dendritic cells; Fungal infection; Glycolysis; Mitochondrial shuttles; Serine biosynthesis |
المشرفين على المادة: | 7440-44-0 (Carbon) 9010-72-4 (Zymosan) 0 (Reactive Oxygen Species) EC 1.6.3.- (NADPH Oxidases) 0 (Cytokines) |
تواريخ الأحداث: | Date Created: 20240514 Date Completed: 20240612 Latest Revision: 20240612 |
رمز التحديث: | 20240613 |
مُعرف محوري في PubMed: | PMC11103932 |
DOI: | 10.1016/j.redox.2024.103187 |
PMID: | 38744190 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2213-2317 |
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DOI: | 10.1016/j.redox.2024.103187 |