دورية أكاديمية
Porcine nasal septum cartilage-derived decellularized matrix promotes chondrogenic differentiation of human umbilical mesenchymal stem cells without exogenous growth factors.
| العنوان: | Porcine nasal septum cartilage-derived decellularized matrix promotes chondrogenic differentiation of human umbilical mesenchymal stem cells without exogenous growth factors. |
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| المؤلفون: | Shen J; Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Guizhou, P. R. China. yan.liang524@hotmail.com.; Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, P. R. China. g_lhpeng@163.com.; Department of Plastic Surgery, Taizhou Enze Medical Center, Zhejiang, P. R. China., Ye D; Research Center for Translational Medicine, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, P. R. China., Jin H; Department of Cardiology, Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, P. R. China., Wu Y; Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, P. R. China. g_lhpeng@163.com., Peng L; Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, P. R. China. g_lhpeng@163.com., Liang Y; Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Guizhou, P. R. China. yan.liang524@hotmail.com. |
| المصدر: | Journal of materials chemistry. B [J Mater Chem B] 2024 Jun 05; Vol. 12 (22), pp. 5513-5524. Date of Electronic Publication: 2024 Jun 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Mesenchymal Stem Cells*/cytology , Mesenchymal Stem Cells*/metabolism , Cell Differentiation* , Chondrogenesis* , Nasal Septum*/cytology , Nasal Septum*/chemistry, Humans ; Animals ; Swine ; Cells, Cultured ; Tissue Scaffolds/chemistry ; Decellularized Extracellular Matrix/chemistry ; Decellularized Extracellular Matrix/pharmacology ; Extracellular Matrix/metabolism ; Extracellular Matrix/chemistry ; Tissue Engineering ; Umbilical Cord/cytology |
| مستخلص: | Background: In the domain of plastic surgery, nasal cartilage regeneration is of significant importance. The extracellular matrix (ECM) from porcine nasal septum cartilage has shown potential for promoting human cartilage regeneration. Nonetheless, the specific biological inductive factors and their pathways in cartilage tissue engineering remain undefined. Methods: The decellularized matrix derived from porcine nasal septum cartilage (PN-DCM) was prepared using a grinding method. Human umbilical cord mesenchymal stem cells (HuMSCs) were cultured on these PN-DCM scaffolds for 4 weeks without exogenous growth factors to evaluate their chondroinductive potential. Subsequently, proteomic analysis was employed to identify potential biological inductive factors within the PN-DCM scaffolds. Results: Compared to the TGF-β3-cultured pellet model serving as a positive control, the PN-DCM scaffolds promoted significant deposition of a Safranin-O positive matrix and Type II collagen by HuMSCs. Gene expression profiling revealed upregulation of ACAN, COL2A1, and SOX9. Proteomic analysis identified potential chondroinductive factors in the PN-DCM scaffolds, including CYTL1, CTGF, MGP, ITGB1, BMP7, and GDF5, which influence HuMSC differentiation. Conclusion: Our findings have demonstrated that the PN-DCM scaffolds promoted HuMSC differentiation towards a nasal chondrocyte phenotype without the supplementation of exogenous growth factors. This outcome is associated with the chondroinductive factors present within the PN-DCM scaffolds. |
| المشرفين على المادة: | 0 (Decellularized Extracellular Matrix) |
| تواريخ الأحداث: | Date Created: 20240515 Date Completed: 20240605 Latest Revision: 20240605 |
| رمز التحديث: | 20240605 |
| DOI: | 10.1039/d3tb03077f |
| PMID: | 38745541 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2050-7518 |
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| DOI: | 10.1039/d3tb03077f |