دورية أكاديمية
Genomic surveillance and serological profile of SARS-CoV-2 variants circulating in Macaé and nearby cities, southeastern Brazil.
العنوان: | Genomic surveillance and serological profile of SARS-CoV-2 variants circulating in Macaé and nearby cities, southeastern Brazil. |
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المؤلفون: | da Silva ACVF; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Silva CAO; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., de Sousa GF; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Coelho VAGS; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., da Cunha LT; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Paes AN; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Pozzobon APB; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., das Graças Dos Santos D; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Carpes RM; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Costa EP; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Monteiro-de-Barros C; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Nepomuceno-Silva JL; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., de Souza Gestinari R; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Mury FB; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil. |
المصدر: | Frontiers in microbiology [Front Microbiol] 2024 Apr 30; Vol. 15, pp. 1386271. Date of Electronic Publication: 2024 Apr 30 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
مستخلص: | Introduction: A characteristic of the COVID-19 pandemic has been the sequential emergence and global dissemination of SARS-CoV-2 variants, noted for their enhanced transmission efficiency. These variants with mutations in the Spike glycoprotein (S-glycoprotein), which interacts with ACE2 receptors in human cells is critical for infection, affects the transmissibility of the virus, which is a matter of great concern for public health. Objective: This research analyses the effects these variants on a cohort of vaccinated and naturally infected individuals from the cities of Macaé-RJ, Rio das Ostras-RJ, and Campos dos Goytacazes-RJ, Brazil, from March 2021 to March 2023. Methods: This investigation encompasses the Alpha (B.1.1.7), Gamma (P.1), Delta (B.1.617.2, B.1.671.3), and Omicron (BQ.1, BQ.1.1 sublines, and BF.7) variants, focusing on their genomic surveillance and implications for the disease's epidemiology. The experimental analysis included a control group (vaccinated and uninfected subjects), and an infected group (post-vaccinated subjects). Samples from nasopharyngeal swabs underwent viral detection via RT-qPCR for diagnosis confirmation. RNase H-dependent RT-qPCR (rhAmp-PCR) and third-generation sequencing were used to detect SARS-CoV-2 variants. Anti-S-glycoprotein immunoglobulins were also evaluated for vaccinated infected and noninfected volunteers. Symptoms from infected individuals were compiled in order to reveal patterns of clinical signs associated with viral infection. Results: The study included 289 participants, with infections identified by Gamma ( n = 44), Delta ( n = 189), and Omicron ( n = 56) variants. The prevalent symptoms among the naturally infected participants were cough, fever, sore throat, headache, and runny nose. For Omicron, cognitive symptoms such as memory loss and concentration issues were reported. Interestingly, the infected vaccinated group had higher anti-S-glycoprotein IgM production ( n = 28, 0.2833 ± 0.09768 OD) compared to the uninfected vaccinated group ( n = 14, 0.1035 ± 0.03625 OD). Conversely, anti-S-glycoprotein IgG production was higher in the control group ( n = 12, 1.770 ± 0.1393 OD) than in the infected vaccinated group ( n = 26, 1.391 ± 0.1563 OD). Conclusion: This comprehensive study enables monitoring of predominant variants and their correlation with clinical cases, providing valuable insights for public health. Our research group continues to survey circulating variants, contributing to the global understanding of the pandemic. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 da Silva, Silva, de Sousa, Coelho, da Cunha, Paes, Pozzobon, das Graças dos Santos, Carpes, Costa, Monteiro-de-Barros, Nepomuceno-Silva, de Souza Gestinari and Mury.) |
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فهرسة مساهمة: | Keywords: COVID-19; SARS-CoV-2; genomic surveillance; serology; variants |
تواريخ الأحداث: | Date Created: 20240515 Latest Revision: 20240516 |
رمز التحديث: | 20240516 |
مُعرف محوري في PubMed: | PMC11091293 |
DOI: | 10.3389/fmicb.2024.1386271 |
PMID: | 38746751 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-302X |
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DOI: | 10.3389/fmicb.2024.1386271 |