دورية أكاديمية
Genomic surveillance and serological profile of SARS-CoV-2 variants circulating in Macaé and nearby cities, southeastern Brazil.
| العنوان: | Genomic surveillance and serological profile of SARS-CoV-2 variants circulating in Macaé and nearby cities, southeastern Brazil. |
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| المؤلفون: | da Silva ACVF; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Silva CAO; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., de Sousa GF; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Coelho VAGS; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., da Cunha LT; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Paes AN; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Pozzobon APB; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., das Graças Dos Santos D; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Carpes RM; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Costa EP; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Monteiro-de-Barros C; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Nepomuceno-Silva JL; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., de Souza Gestinari R; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil., Mury FB; Laboratório Integrado de Doenças Emergentes e Negligenciadas, Instituto de Biodiversidade e Sustentabilidade (NUPEM) - Universidade Federal do Rio de Janeiro (UFRJ), Macaé, Brazil. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2024 Apr 30; Vol. 15, pp. 1386271. Date of Electronic Publication: 2024 Apr 30 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Introduction: A characteristic of the COVID-19 pandemic has been the sequential emergence and global dissemination of SARS-CoV-2 variants, noted for their enhanced transmission efficiency. These variants with mutations in the Spike glycoprotein (S-glycoprotein), which interacts with ACE2 receptors in human cells is critical for infection, affects the transmissibility of the virus, which is a matter of great concern for public health. Objective: This research analyses the effects these variants on a cohort of vaccinated and naturally infected individuals from the cities of Macaé-RJ, Rio das Ostras-RJ, and Campos dos Goytacazes-RJ, Brazil, from March 2021 to March 2023. Methods: This investigation encompasses the Alpha (B.1.1.7), Gamma (P.1), Delta (B.1.617.2, B.1.671.3), and Omicron (BQ.1, BQ.1.1 sublines, and BF.7) variants, focusing on their genomic surveillance and implications for the disease's epidemiology. The experimental analysis included a control group (vaccinated and uninfected subjects), and an infected group (post-vaccinated subjects). Samples from nasopharyngeal swabs underwent viral detection via RT-qPCR for diagnosis confirmation. RNase H-dependent RT-qPCR (rhAmp-PCR) and third-generation sequencing were used to detect SARS-CoV-2 variants. Anti-S-glycoprotein immunoglobulins were also evaluated for vaccinated infected and noninfected volunteers. Symptoms from infected individuals were compiled in order to reveal patterns of clinical signs associated with viral infection. Results: The study included 289 participants, with infections identified by Gamma ( n = 44), Delta ( n = 189), and Omicron ( n = 56) variants. The prevalent symptoms among the naturally infected participants were cough, fever, sore throat, headache, and runny nose. For Omicron, cognitive symptoms such as memory loss and concentration issues were reported. Interestingly, the infected vaccinated group had higher anti-S-glycoprotein IgM production ( n = 28, 0.2833 ± 0.09768 OD) compared to the uninfected vaccinated group ( n = 14, 0.1035 ± 0.03625 OD). Conversely, anti-S-glycoprotein IgG production was higher in the control group ( n = 12, 1.770 ± 0.1393 OD) than in the infected vaccinated group ( n = 26, 1.391 ± 0.1563 OD). Conclusion: This comprehensive study enables monitoring of predominant variants and their correlation with clinical cases, providing valuable insights for public health. Our research group continues to survey circulating variants, contributing to the global understanding of the pandemic. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 da Silva, Silva, de Sousa, Coelho, da Cunha, Paes, Pozzobon, das Graças dos Santos, Carpes, Costa, Monteiro-de-Barros, Nepomuceno-Silva, de Souza Gestinari and Mury.) |
| References: | Front Immunol. 2021 Nov 30;12:751778. (PMID: 34917076) Clin Chim Acta. 2020 Aug;507:164-166. (PMID: 32343948) Nature. 2021 Mar;591(7851):639-644. (PMID: 33461210) Biochem Eng J. 2022 Aug;186:108537. (PMID: 35874089) Euro Surveill. 2021 Jan;26(1):. (PMID: 33413740) mBio. 2022 Apr 26;13(2):e0297921. (PMID: 35352979) Nat Commun. 2023 Jul 26;14(1):4485. (PMID: 37495586) JAMA. 2021 Apr 6;325(13):1261-1262. (PMID: 33571363) Rev Med Virol. 2021 May;31(3):e2181. (PMID: 33152146) Physiol Res. 2020 Jul 16;69(3):379-388. (PMID: 32469225) J Infect Public Health. 2022 Feb;15(2):228-240. (PMID: 35042059) Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168) BMC Infect Dis. 2023 Nov 28;23(1):838. (PMID: 38017395) Wien Klin Wochenschr. 2021 Apr;133(7-8):271-283. (PMID: 33725201) Elife. 2020 Oct 28;9:. (PMID: 33112236) J Med Virol. 2022 Mar;94(3):1115-1129. (PMID: 34726786) N Engl J Med. 2020 Oct 29;383(18):1724-1734. (PMID: 32871063) Semin Immunol. 2021 Jun;55:101533. (PMID: 34836774) Euro Surveill. 2020 Jan;25(3):. (PMID: 31992387) Presse Med. 2022 Sep;51(3):104131. (PMID: 35667598) Nat Rev Microbiol. 2021 Jul;19(7):409-424. (PMID: 34075212) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) China CDC Wkly. 2021 Dec 3;3(49):1049-1051. (PMID: 34934514) Nat Med. 2020 Jun;26(6):845-848. (PMID: 32350462) Exp Mol Med. 2021 Apr;53(4):537-547. (PMID: 33864026) Cell. 2020 Aug 20;182(4):812-827.e19. (PMID: 32697968) Microb Biotechnol. 2020 Jul;13(4):950-961. (PMID: 32333644) Clin Microbiol Infect. 2021 Jan 20;:. (PMID: 33482352) Nat Rev Genet. 2021 Dec;22(12):757-773. (PMID: 34535792) Cell. 2022 Mar 3;185(5):860-871.e13. (PMID: 35120603) Proc Natl Acad Sci U S A. 2021 Sep 7;118(36):. (PMID: 34417349) J Med Virol. 2022 Mar;94(3):847-857. (PMID: 34609003) PLoS One. 2021 Dec 15;16(12):e0260487. (PMID: 34910739) Cureus. 2021 Mar 3;13(3):e13681. (PMID: 33833907) Vaccines (Basel). 2021 Jan 11;9(1):. (PMID: 33440640) Viruses. 2023 Jul 13;15(7):. (PMID: 37515231) Cell Host Microbe. 2021 Mar 10;29(3):477-488.e4. (PMID: 33535027) Front Immunol. 2020 Aug 26;11:2037. (PMID: 32983152) Salud Publica Mex. 2023 Dec 08;66(1, ene-feb):14-16. (PMID: 38065105) J Virol. 2020 Mar 17;94(7):. (PMID: 31996437) Rev Med Virol. 2022 Jul;32(4):e2313. (PMID: 34755408) Clinics (Sao Paulo). 2023 Jun 13;78:100233. (PMID: 37348256) Epidemiol Infect. 2022 Nov 03;150:e187. (PMID: 36325837) Science. 2021 May 21;372(6544):815-821. (PMID: 33853970) Nat Commun. 2021 Oct 29;12(1):6250. (PMID: 34716320) Lancet. 2021 Jan 23;397(10271):267. (PMID: 33485437) Sci Rep. 2021 Oct 11;11(1):20121. (PMID: 34635707) J Gene Med. 2022 Jul;24(7):e3435. (PMID: 35726542) Clin Microbiol Infect. 2020 Aug;26(8):1094.e7-1094.e10. (PMID: 32502646) Nature. 2022 Feb;602(7898):657-663. (PMID: 35016194) J Clin Med Res. 2021 Jun;13(6):317-325. (PMID: 34267839) Sci Immunol. 2020 Oct 8;5(52):. (PMID: 33033173) Emerg Infect Dis. 2020 Jul;26(7):1478-1488. (PMID: 32267220) Basic Res Cardiol. 2020 Apr 9;115(3):31. (PMID: 32274570) J Infect Public Health. 2023 Jan;16(1):4-14. (PMID: 36446204) Cell. 2020 Oct 29;183(3):739-751.e8. (PMID: 32991842) Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943) Clin Microbiol Infect. 2021 Jul;27(7):981-986. (PMID: 33975005) Ann Med. 2022 Dec;54(1):524-540. (PMID: 35132910) PLoS One. 2021 Mar 11;16(3):e0247773. (PMID: 33705435) Saudi J Biol Sci. 2022 May;29(5):3586-3599. (PMID: 35165505) Front Public Health. 2023 Sep 13;11:1256768. (PMID: 37780420) |
| فهرسة مساهمة: | Keywords: COVID-19; SARS-CoV-2; genomic surveillance; serology; variants |
| تواريخ الأحداث: | Date Created: 20240515 Latest Revision: 20240516 |
| رمز التحديث: | 20240516 |
| مُعرف محوري في PubMed: | PMC11091293 |
| DOI: | 10.3389/fmicb.2024.1386271 |
| PMID: | 38746751 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2024.1386271 |