دورية أكاديمية
Small extracellular vesicles detection using dielectrophoresis-based microfluidic chip for diagnosis of breast cancer.
| العنوان: | Small extracellular vesicles detection using dielectrophoresis-based microfluidic chip for diagnosis of breast cancer. |
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| المؤلفون: | Lan M; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China., Ren Z; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China., Cheng C; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China., Li G; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China. Electronic address: ligy@jlu.edu.cn., Yang F; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China. Electronic address: fangyang@jlu.edu.cn. |
| المصدر: | Biosensors & bioelectronics [Biosens Bioelectron] 2024 Sep 01; Vol. 259, pp. 116382. Date of Electronic Publication: 2024 May 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| مواضيع طبية MeSH: | Breast Neoplasms*/diagnosis , Breast Neoplasms*/blood , Extracellular Vesicles*/chemistry , Biosensing Techniques*/instrumentation , Biosensing Techniques*/methods , Mucin-1*/blood , Mucin-1*/analysis , Biomarkers, Tumor*/blood , Biomarkers, Tumor*/isolation & purification , Epithelial Cell Adhesion Molecule* , Lab-On-A-Chip Devices*, Humans ; Female ; Limit of Detection ; Equipment Design ; Electrophoresis/instrumentation ; Microfluidic Analytical Techniques/instrumentation ; Liquid Biopsy/methods ; Liquid Biopsy/instrumentation |
| مستخلص: | Small extracellular vesicles (sEVs) reflect the genotype and phenotype of original cells and are biomarkers for early diagnosis and treatment monitoring of tumors. Yet, their small size and low density make them difficult to isolate and detect in body fluid samples. This study proposes a novel acDEP-Exo chip filled with transparent micro-beads, which formed a non-uniform electrical field, and finally achieved rapid, sensitive, and tunable sEVs capture and detection. The method requires only 20-50 μL of sample, achieved a limit of detection (LOD) of 161 particles/μL, and can detect biomarkers within 13 min. We applied the chip to analyze the two markers of sEV's EpCAM and MUC1 in clinical plasma samples from breast cancer (BC) patients and healthy volunteers and found that the combined evaluation of sEV's biomarkers has extremely high sensitivity, specificity and accuracy. The present study introduces an alternative approach to sEVs isolation and detection, has a great potential in real-time sEVs-based liquid biopsy. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biomarkers; Breast cancer; Dielectrophoresis; Liquid biopsy; Microfluidics; Small extracellular vesicles |
| المشرفين على المادة: | 0 (Mucin-1) 0 (Biomarkers, Tumor) 0 (Epithelial Cell Adhesion Molecule) 0 (MUC1 protein, human) 0 (EPCAM protein, human) |
| تواريخ الأحداث: | Date Created: 20240515 Date Completed: 20240601 Latest Revision: 20240601 |
| رمز التحديث: | 20240602 |
| DOI: | 10.1016/j.bios.2024.116382 |
| PMID: | 38749284 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2024.116382 |