دورية أكاديمية
أعرض في EDS

FTO-mediated regulation of m6A methylation is closely related to apoptosis induced by repeated UV irradiation.

التفاصيل البيبلوغرافية
العنوان: FTO-mediated regulation of m6A methylation is closely related to apoptosis induced by repeated UV irradiation.
المؤلفون: Lin Y; Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Sun Y; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Hou W; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Chen X; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Zhou F; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Xu Q; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address: xqf69@163.com., Zheng Y; Nanfang Hospital, Southern Medical University, Guangzhou, China; The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address: benbenzhu-11@163.com.
المصدر: Journal of dermatological science [J Dermatol Sci] 2024 Jun; Vol. 114 (3), pp. 124-132. Date of Electronic Publication: 2024 Jan 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9011485 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-569X (Electronic) Linking ISSN: 09231811 NLM ISO Abbreviation: J Dermatol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier, c1990-
مواضيع طبية MeSH: Ultraviolet Rays*/adverse effects , Alpha-Ketoglutarate-Dependent Dioxygenase FTO*/metabolism , Alpha-Ketoglutarate-Dependent Dioxygenase FTO*/genetics , Apoptosis*/radiation effects , Apoptosis*/genetics , Adenosine*/analogs & derivatives , Adenosine*/metabolism , HaCaT Cells* , Mice, Nude* , Mice, Inbred BALB C* , Skin Aging*/radiation effects , Skin Aging*/genetics, Animals ; Humans ; Mice ; Methylation/radiation effects ; Skin/radiation effects ; Skin/pathology ; Skin/metabolism ; Keratinocytes/radiation effects ; Keratinocytes/metabolism ; Cell Survival/radiation effects ; Epigenesis, Genetic/radiation effects ; Female
مستخلص: Background: Ultraviolet (UV) damage is closely related to skin photoaging and many skin diseases, including dermatic tumors. N6-methyladenosine (m6A) modification is an important epigenetic regulatory mechanism. However, the role of m6A methylation in apoptosis induced by repeated UV irradiation has not been characterized.
Objective: To explore m6A methylation changes and regulatory mechanisms in the repeated UV-induced skin damage process, especially apoptosis.
Methods: HaCaT cells and BALB/c-Nu nude mice were exposed to repeated UVB/UVA+UVB irradiation. Colorimetry and flow cytometry were used to measure cellular viability and apoptosis. m6A-modified genes were detected via colorimetry and methylated RNA immunoprecipitation (MeRIP) sequencing. Methyltransferases and demethylases were detected via RT-PCR, western blotting and immunohistochemistry. Transfection of siRNA and plasmid was performed to knock down or overexpress the selected genes.
Results: After UVB irradiation, 861 m6A peaks were increased and 425 m6A peaks were decreased in HaCaT cells. The differentially modified genes were enriched in apoptosis-related pathways. The m6A demethylase FTO was decreased in both HaCaT cells and mouse skin after UV damage. Overexpressing FTO could improve cell viability, inhibit apoptosis and decrease RNA-m6A methylation, including LPCAT3-m6A, which increase LPCAT3 expression, cell viability promotion and apoptosis inhibition.
Conclusion: Our study identified the cell m6A methylation change lists after repeated UVB irradiation, and revealed that FTO and LPCAT3 play key roles in the m6A methylation pathogenesis of UV-induced skin cell apoptosis. FTO-m6A-LPCAT3 might serve as a novel upstream target for preventing and treating photoaging and UV-induced skin diseases.
Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Apoptosis; FTO; N6-methyladenosine (m6A); Skin; Ultraviolet (UV)
المشرفين على المادة: EC 1.14.11.33 (Alpha-Ketoglutarate-Dependent Dioxygenase FTO)
K72T3FS567 (Adenosine)
CLE6G00625 (N-methyladenosine)
EC 1.14.11.33 (FTO protein, human)
EC 1.14.11.- (FTO protein, mouse)
تواريخ الأحداث: Date Created: 20240515 Date Completed: 20240621 Latest Revision: 20240710
رمز التحديث: 20240710
DOI: 10.1016/j.jdermsci.2024.01.001
PMID: 38749796
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-569X
DOI:10.1016/j.jdermsci.2024.01.001