دورية أكاديمية
Ascertaining variations in the activity of larval midgut enzymes of Helicoverpa armigera by dietary emamectin benzoate through biochemical and in silico docking study.
| العنوان: | Ascertaining variations in the activity of larval midgut enzymes of Helicoverpa armigera by dietary emamectin benzoate through biochemical and in silico docking study. |
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| المؤلفون: | Dagar VS; Department of Zoology, Acharya Narendra Dev College (University of Delhi), Govindpuri, New Delhi, India; Department of Zoology, Deen Dayal Upadhyaya College, University of Delhi, Sector-3, Dwarka, New Delhi, India. Electronic address: vinaydagar04@gmail.com., Mishra M; Department of Zoology, Acharya Narendra Dev College (University of Delhi), Govindpuri, New Delhi, India. Electronic address: mishra_monika7@yahoo.co.in., Sharma A; Galgotias University, School of Biological and Life Sciences, Greater Noida, Uttar Pradesh, India. Electronic address: aartisharma@galgotiasuniversity.edu.in., Sankar M; Department of Zoology, Acharya Narendra Dev College (University of Delhi), Govindpuri, New Delhi, India. Electronic address: sankarmanu00@gmail.com., Goyal S; Department of Microbiology, University of Manitoba, Winnipeg City, Manitoba Province, Canada. Electronic address: Sggoyal742@gmail.com., Pal R; Department of Biotechnology, University of Wroclaw, Wroclaw, Poland. Electronic address: paulranjan843@gmail.com., Kumar S; Department of Zoology, Acharya Narendra Dev College (University of Delhi), Govindpuri, New Delhi, India. Electronic address: saritakumar@andc.du.ac.in. |
| المصدر: | Chemosphere [Chemosphere] 2024 Jul; Vol. 359, pp. 142288. Date of Electronic Publication: 2024 May 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Science Ltd Original Publication: Oxford, New York, : Pergamon Press. |
| مواضيع طبية MeSH: | Ivermectin*/analogs & derivatives , Ivermectin*/toxicity , Larva*/drug effects , Molecular Docking Simulation* , Moths*/drug effects , Insecticides*/toxicity , Insecticides*/chemistry , Insecticides*/metabolism , Alkaline Phosphatase*/metabolism , Acid Phosphatase*/metabolism , Alanine Transaminase*/metabolism , Aspartate Aminotransferases*/metabolism, Animals ; Helicoverpa armigera |
| مستخلص: | Helicoverpa armigera, a ubiquitous polyphagous pest, poses a significant threat to global agriculture, causing substantial economic losses and demonstrating resistance to synthetic pesticides. This study investigates the potential of emamectin benzoate (EMB), an avermectin derivative, as an effective control agent against H. armigera. The larvae of the NBII-MP-NOC-01 strain of H. armigera were reared on an artificial diet. The impact of dietary EMB was examined on four midgut enzymes; alanine aminotransferase (ALT), aspartate aminotransferase (AST), acid phosphatase (ACP), and alkaline phosphatase (ALP). Results showed a dose-dependent and time-dependent reduction in ALT and AST activity, while an initial increase and subsequent decline in ACP and ALP activity at higher EMB concentrations. Computational modelling of enzyme structures and molecular docking studies revealed differential binding of EMB with the midgut enzymes. The strongest interaction was observed between EMB and ALT residues, contrasting with weakest interactions observed with AST. The study also showed that decreased activity of transaminases in H. armigera caused by EMB may be because of stability-activity trade-off, while in phosphatases reverse may be the case. This research provides crucial insights into the biochemical responses and the intricate insecticide-enzyme interactions in H. armigera caused by EMB exposure. This study lays the foundation for further research aimed at developing environmentally friendly approaches for managing H. armigera, addressing the challenges associated with conventional pesticides. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Emamectin benzoate; Helicoverpa armigera; Insecticide resistance; Midgut enzymes; Pest management |
| المشرفين على المادة: | 70288-86-7 (Ivermectin) HVM3G4A01W (emamectin benzoate) 0 (Insecticides) EC 3.1.3.1 (Alkaline Phosphatase) EC 3.1.3.2 (Acid Phosphatase) EC 2.6.1.2 (Alanine Transaminase) EC 2.6.1.1 (Aspartate Aminotransferases) |
| تواريخ الأحداث: | Date Created: 20240516 Date Completed: 20240604 Latest Revision: 20240604 |
| رمز التحديث: | 20240604 |
| DOI: | 10.1016/j.chemosphere.2024.142288 |
| PMID: | 38750729 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1298 |
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| DOI: | 10.1016/j.chemosphere.2024.142288 |