دورية أكاديمية
أعرض في EDS

Lipid levels correlate with neuronal and dopaminergic markers during the differentiation of SH-SY5Y cells.

التفاصيل البيبلوغرافية
العنوان: Lipid levels correlate with neuronal and dopaminergic markers during the differentiation of SH-SY5Y cells.
المؤلفون: Marlet FR; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Muñoz SS; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Sotiraki N; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Eliasen JN; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Woessmann J; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby 2800, Denmark., Weicher J; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Dreier JE; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Schoof EM; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby 2800, Denmark., Kohlmeier KA; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark., Maeda K; Cell Death and Metabolism group, Center for Autophagy, Recycling and Disease, Danish Cancer Institute, Copenhagen, Denmark., Galvagnion C; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark. Electronic address: celine.galvagnion@sund.ku.dk.
المصدر: Biochimica et biophysica acta. Molecular basis of disease [Biochim Biophys Acta Mol Basis Dis] 2024 Aug; Vol. 1870 (6), pp. 167212. Date of Electronic Publication: 2024 May 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731730 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-260X (Electronic) Linking ISSN: 09254439 NLM ISO Abbreviation: Biochim Biophys Acta Mol Basis Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Cell Differentiation* , Dopaminergic Neurons*/metabolism, Humans ; Cell Line, Tumor ; Lipidomics/methods ; alpha-Synuclein/metabolism ; Neuroblastoma/metabolism ; Neuroblastoma/pathology ; Parkinson Disease/metabolism ; Parkinson Disease/pathology ; Lipid Metabolism ; Biomarkers/metabolism ; Lipids/analysis ; Sphingolipids/metabolism ; Proteomics/methods
مستخلص: Parkinson's Disease (PD) is characterised by the loss of dopaminergic neurons and the deposition of protein inclusions called Lewy Bodies (LBs). LBs are heterogeneous structures composed of protein and lipid molecules and their main constituent is the presynaptic protein α-synuclein. SH-SY5Y cells are neuroblastoma cells commonly used to model PD because they express dopaminergic markers and α-synuclein and they can be differentiated into neuronal cells using established protocols. Despite increasing evidence pointing towards a role of lipids in PD, limited knowledge is available on the lipidome of undifferentiated and differentiated SH-SY5Y cells. Using a combination of lipidomics, proteomics, morphological and electrophysiological measurements, we identified specific lipids, including sphingolipids, whose levels are affected by the differentiation of SH-SY5Y neuroblastoma cells and found that the levels of these lipids correlate with those of neuronal and dopaminergic markers. These results provide a quantitative characterisation of the changes in lipidome associated with the differentiation of SH-SY5Y cells into more neuronal and dopaminergic-like phenotype and serve as a basis for further characterisation of lipid disruptions in association with PD and its risk factors in this dopaminergic-like neuronal cell model.
Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest with the contents of this article.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Electrophysiology; Lipidomics; Neuroblastoma cells; Proteomics; Sphingolipids
المشرفين على المادة: 0 (alpha-Synuclein)
0 (Biomarkers)
0 (Lipids)
0 (Sphingolipids)
تواريخ الأحداث: Date Created: 20240516 Date Completed: 20240615 Latest Revision: 20240615
رمز التحديث: 20240616
DOI: 10.1016/j.bbadis.2024.167212
PMID: 38750771
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-260X
DOI:10.1016/j.bbadis.2024.167212