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Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking.

التفاصيل البيبلوغرافية
العنوان: Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking.
المؤلفون: Bonazza S; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom., Coutts HL; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom., Sukumar S; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom., Turkington HL; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom., Courtney DG; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom.
المصدر: PLoS pathogens [PLoS Pathog] 2024 May 16; Vol. 20 (5), pp. e1012231. Date of Electronic Publication: 2024 May 16 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Influenza A virus*/genetics , Influenza, Human*/metabolism , Influenza, Human*/virology , Influenza, Human*/genetics , RNA, Messenger*/metabolism , RNA, Messenger*/genetics , RNA, Viral*/metabolism , RNA, Viral*/genetics , RNA-Binding Proteins*/metabolism , RNA-Binding Proteins*/genetics, Animals ; Humans ; Active Transport, Cell Nucleus ; Cell Nucleus/metabolism ; Cell Nucleus/virology ; RNA Transport ; Virus Replication
مستخلص: Utilisation of RNA-binding proteins (RBPs) is an important aspect of post-transcriptional regulation of viral RNA. Viruses such as influenza A viruses (IAV) interact with RBPs to regulate processes including splicing, nuclear export and trafficking, while also encoding RBPs within their genomes, such as NP and NS1. But with almost 1000 RBPs encoded within the human genome it is still unclear what role, if any, many of these proteins play during viral replication. Using the RNA interactome capture (RIC) technique, we isolated RBPs from IAV infected cells to unravel the RBPome of mRNAs from IAV infected human cells. This led to the identification of one particular RBP, MKRN2, that associates with and positively regulates IAV mRNA. Through further validation, we determined that MKRN2 is involved in the nuclear-cytoplasmic trafficking of IAV mRNA potentially through an association with the RNA export mediator GLE1. In the absence of MKRN2, IAV mRNAs accumulate in the nucleus of infected cells, which may lead to their degradation by the nuclear RNA exosome complex. MKRN2, therefore, appears to be required for the efficient nuclear export of IAV mRNAs in human cells.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Bonazza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (RNA, Messenger)
0 (RNA, Viral)
0 (RNA-Binding Proteins)
0 (MKRN2 protein, human)
تواريخ الأحداث: Date Created: 20240516 Date Completed: 20240529 Latest Revision: 20240605
رمز التحديث: 20240605
مُعرف محوري في PubMed: PMC11135703
DOI: 10.1371/journal.ppat.1012231
PMID: 38753876
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7374
DOI:10.1371/journal.ppat.1012231