دورية أكاديمية
Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacers in trans.
| العنوان: | Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacers in trans. |
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| المؤلفون: | Benz F; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Synthetic Biology, Paris 75015, France; Institut Pasteur, Université Paris Cité, CNRS UMR3525, Microbial Evolutionary Genomics, Paris 75015, France; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark; Institute of Integrative Biology, Department of Environmental Systems Science, ETH Zurich, Zurich, Switzerland., Camara-Wilpert S; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Russel J; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Wandera KG; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany., Čepaitė R; Life Sciences Center - European Molecular Biology Laboratory (LSC-EMBL) Partnership for Genome Editing Technologies, Vilnius University - Life Sciences Center, Vilnius University, Vilnius 10257, Lithuania., Ares-Arroyo M; Institut Pasteur, Université Paris Cité, CNRS UMR3525, Microbial Evolutionary Genomics, Paris 75015, France., Gomes-Filho JV; Department of Biology, Philipps Universität Marburg, Marburg, Germany., Englert F; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany., Kuehn JA; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Gloor S; Institute of Integrative Biology, Department of Environmental Systems Science, ETH Zurich, Zurich, Switzerland., Mestre MR; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Cuénod A; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland; Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland; Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada., Aguilà-Sans M; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Maccario L; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Egli A; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland; Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland., Randau L; Department of Biology, Philipps Universität Marburg, Marburg, Germany; SYNMIKRO, Center for Synthetic Microbiology, Marburg, Germany., Pausch P; Life Sciences Center - European Molecular Biology Laboratory (LSC-EMBL) Partnership for Genome Editing Technologies, Vilnius University - Life Sciences Center, Vilnius University, Vilnius 10257, Lithuania., Rocha EPC; Institut Pasteur, Université Paris Cité, CNRS UMR3525, Microbial Evolutionary Genomics, Paris 75015, France., Beisel CL; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany; Medical Faculty, University of Würzburg, Würzburg, Germany., Madsen JS; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark., Bikard D; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Synthetic Biology, Paris 75015, France., Hall AR; Institute of Integrative Biology, Department of Environmental Systems Science, ETH Zurich, Zurich, Switzerland., Sørensen SJ; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark. Electronic address: sjs@bio.ku.dk., Pinilla-Redondo R; Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark. Electronic address: rafael.pinilla@bio.ku.dk. |
| المصدر: | Cell host & microbe [Cell Host Microbe] 2024 Jun 12; Vol. 32 (6), pp. 875-886.e9. Date of Electronic Publication: 2024 May 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press |
| مواضيع طبية MeSH: | CRISPR-Cas Systems* , Plasmids*/genetics , Klebsiella pneumoniae*/genetics , Conjugation, Genetic*, Clustered Regularly Interspaced Short Palindromic Repeats ; Gene Transfer, Horizontal ; Bacteriophages/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism |
| مستخلص: | Plasmid-encoded type IV-A CRISPR-Cas systems lack an acquisition module, feature a DinG helicase instead of a nuclease, and form ribonucleoprotein complexes of unknown biological functions. Type IV-A3 systems are carried by conjugative plasmids that often harbor antibiotic-resistance genes and their CRISPR array contents suggest a role in mediating inter-plasmid conflicts, but this function remains unexplored. Here, we demonstrate that a plasmid-encoded type IV-A3 system co-opts the type I-E adaptation machinery from its host, Klebsiella pneumoniae (K. pneumoniae), to update its CRISPR array. Furthermore, we reveal that robust interference of conjugative plasmids and phages is elicited through CRISPR RNA-dependent transcriptional repression. By silencing plasmid core functions, type IV-A3 impacts the horizontal transfer and stability of targeted plasmids, supporting its role in plasmid competition. Our findings shed light on the mechanisms and ecological function of type IV-A3 systems and demonstrate their practical efficacy for countering antibiotic resistance in clinically relevant strains. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CRISPR-Cas; DinG helicase; Klebsiella pneumoniae; adaptive immunity; antibiotic resistance; inter-plasmid competition; phages; plasmids; type IV CRISPR-Cas |
| المشرفين على المادة: | 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20240516 Date Completed: 20240613 Latest Revision: 20240627 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1016/j.chom.2024.04.016 |
| PMID: | 38754416 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1934-6069 |
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| DOI: | 10.1016/j.chom.2024.04.016 |