دورية أكاديمية
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Protective effect of luteolin against oxidative stress‑mediated cell injury via enhancing antioxidant systems.

التفاصيل البيبلوغرافية
العنوان: Protective effect of luteolin against oxidative stress‑mediated cell injury via enhancing antioxidant systems.
المؤلفون: Fernando PDSM; Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea., Ko DO; Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea., Piao MJ; Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea., Kang KA; Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea., Herath HMUL; Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea., Hyun JW; Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea.
المصدر: Molecular medicine reports [Mol Med Rep] 2024 Jul; Vol. 30 (1). Date of Electronic Publication: 2024 May 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Athens, Greece : D. A. Spandidos
مواضيع طبية MeSH: Luteolin*/pharmacology , Oxidative Stress*/drug effects , Antioxidants*/pharmacology , Hydrogen Peroxide*/pharmacology , Hydrogen Peroxide*/toxicity , Reactive Oxygen Species*/metabolism , Apoptosis*/drug effects , Cell Survival*/drug effects , Fibroblasts*/metabolism , Fibroblasts*/drug effects, Animals ; Cell Line ; Cricetinae ; Lipid Peroxidation/drug effects ; Cricetulus
مستخلص: Physiological stress such as excessive reactive oxygen species (ROS) production may contribute normal fibroblasts activation into cancer‑associated fibroblasts, which serve a crucial role in certain types of cancer such as pancreatic, breast, liver and lung cancer. The present study aimed to examine the cytoprotective effects of luteolin (3',4',5,7‑tetrahydroxyflavone) against hydrogen peroxide (H 2 O 2 )‑generated oxidative stress in lung fibroblasts. To examine the effects of luteolin against H 2 O 2 ‑induced damages, cell viability, sub‑G 1 cell population, nuclear staining with Hoechst 33342, lipid peroxidation and comet assays were performed. To evaluate the effects of luteolin on the protein expression level of apoptosis, western blot assay was performed. To assess the antioxidant effects of luteolin, detection of ROS using H 2 DCFDA staining, O 2 ‑ and ·OH using electron spin resonance spectrometer and antioxidant enzyme activity was performed. In a cell‑free chemical system, luteolin scavenges superoxide anion and hydroxyl radical generated by xanthine/xanthine oxidase and the Fenton reaction (FeSO 4 /H 2 O 2 ). Furthermore, Chinese hamster lung fibroblasts (V79‑4) treated with H 2 O 2 showed a significant increase in cellular ROS. Intracellular ROS levels and damage to cellular components such as lipids and DNA in H 2 O 2 ‑treated cells were significantly decreased by luteolin pretreatment. Luteolin increased cell viability, which was impaired following H 2 O 2 treatment and prevented H 2 O 2 ‑mediated apoptosis. Luteolin suppressed active caspase‑9 and caspase‑3 levels while increasing Bcl‑2 expression and decreasing Bax protein levels. Additionally, luteolin restored levels of glutathione that was reduced in response to H 2 O 2 . Moreover, luteolin enhanced the activity and protein expressions of superoxide dismutase, catalase, glutathione peroxidase, and heme oxygenase‑1. Overall, these results indicated that luteolin inhibits H 2 O 2 ‑mediated cellular damage by upregulating antioxidant enzymes.
References: Cell Death Dis. 2019 Mar 4;10(3):218. (PMID: 30833546)
Int J Physiol Pathophysiol Pharmacol. 2022 Aug 15;14(4):276-280. (PMID: 36161255)
Mar Drugs. 2019 Feb 25;17(2):. (PMID: 30823522)
Biochim Biophys Acta Mol Basis Dis. 2020 Jan 1;1866(1):165575. (PMID: 31666206)
Oncol Lett. 2021 May;21(5):413. (PMID: 33841574)
Antioxidants (Basel). 2020 Nov 19;9(11):. (PMID: 33228260)
Front Genet. 2021 Jul 29;12:673002. (PMID: 34394183)
Int J Mol Med. 2015 Feb;35(2):405-12. (PMID: 25435295)
Mol Nutr Food Res. 2024 Jan;68(1):e2300126. (PMID: 38037466)
Oxid Med Cell Longev. 2019 Oct 13;2019:5080843. (PMID: 31737171)
Mol Cell Biochem. 2019 Jan;450(1-2):135-147. (PMID: 29938378)
J Transl Med. 2015 Feb 01;13:42. (PMID: 25638174)
Biotechnol Lett. 2022 Jan;44(1):1-22. (PMID: 34734354)
Exp Mol Med. 2019 Apr 15;51(4):1-14. (PMID: 30988303)
Mol Med Rep. 2021 Feb;23(2):. (PMID: 33355379)
Vitam Horm. 2023;121:109-141. (PMID: 36707132)
Antioxidants (Basel). 2021 Aug 09;10(8):. (PMID: 34439516)
Nat Rev Immunol. 2021 Jul;21(7):411-425. (PMID: 33514947)
Oxid Med Cell Longev. 2018 Feb 11;2018:5730395. (PMID: 29599897)
Arch Biochem Biophys. 2020 Sep 30;691:108512. (PMID: 32712291)
Life Sci. 2016 Jan 1;144:138-47. (PMID: 26656468)
Chem Biol Interact. 2010 May 14;185(3):215-26. (PMID: 20188716)
Int J Oncol. 2017 Oct;51(4):1169-1178. (PMID: 28791416)
Phytomedicine. 2019 Jun;59:152774. (PMID: 31009852)
Int J Mol Sci. 2019 Feb 15;20(4):. (PMID: 30769918)
Molecules. 2020 Nov 11;25(22):. (PMID: 33187049)
Antioxidants (Basel). 2021 Aug 31;10(9):. (PMID: 34573030)
Am J Physiol Lung Cell Mol Physiol. 2017 Nov 1;313(5):L916-L929. (PMID: 28798253)
Biochemistry (Mosc). 2021 Oct;86(10):1256-1274. (PMID: 34903155)
Nat Rev Cancer. 2020 Mar;20(3):174-186. (PMID: 31980749)
Front Pharmacol. 2020 Jan 21;10:1551. (PMID: 32038239)
J Inorg Biochem. 2018 Feb;179:24-31. (PMID: 29156292)
Molecules. 2022 Jul 27;27(15):. (PMID: 35956749)
Biomed Pharmacother. 2019 Apr;112:108612. (PMID: 30798142)
J Ovarian Res. 2018 Nov 19;11(1):93. (PMID: 30454003)
Molecules. 2021 Sep 04;26(17):. (PMID: 34500810)
Int J Med Sci. 2020 Jan 1;17(1):63-70. (PMID: 31929739)
Antioxidants (Basel). 2023 Sep 06;12(9):. (PMID: 37760031)
Oxid Med Cell Longev. 2017;2017:8416763. (PMID: 28819546)
Antioxidants (Basel). 2020 Aug 25;9(9):. (PMID: 32854434)
Arch Toxicol. 2020 Jul;94(7):2293-2317. (PMID: 32524152)
فهرسة مساهمة: Keywords: antioxidant enzyme; apoptosis; lung fibroblast; luteolin; oxidative stress
تواريخ الأحداث: Date Created: 20240517 Date Completed: 20240517 Latest Revision: 20240529
رمز التحديث: 20240529
مُعرف محوري في PubMed: PMC11129544
DOI: 10.3892/mmr.2024.13244
PMID: 38757300
قاعدة البيانات: MEDLINE
الوصف
تدمد:1791-3004
DOI:10.3892/mmr.2024.13244