دورية أكاديمية
Antibody Isolation in C. neoformans.
| العنوان: | Antibody Isolation in C. neoformans. |
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| المؤلفون: | Mendoza SR; Laboratório de Bioquímica e Imunologia das Micoses, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Fluminense Federal University, Niterói, RJ, Brazil.; Pós-Graduação em Imunologia e Inflamação, Instituto de Microbiologia Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., da Silva Ferreira M; Laboratório de Bioquímica e Imunologia das Micoses, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Fluminense Federal University, Niterói, RJ, Brazil.; Pós-Graduação em Imunologia e Inflamação, Instituto de Microbiologia Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Valente MR; Laboratório de Bioquímica e Imunologia das Micoses, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Fluminense Federal University, Niterói, RJ, Brazil.; Programa de Pós-Graduação em Microbiologia e Parasitologia Aplicadas, Instituto Biomédico, Fluminense Federal University, Niterói, RJ, Brazil., Guimarães AJ; Laboratório de Bioquímica e Imunologia das Micoses, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Fluminense Federal University, Niterói, RJ, Brazil. allanguimaraes@id.uff.br.; Pós-Graduação em Imunologia e Inflamação, Instituto de Microbiologia Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. allanguimaraes@id.uff.br.; Programa de Pós-Graduação em Microbiologia e Parasitologia Aplicadas, Instituto Biomédico, Fluminense Federal University, Niterói, RJ, Brazil. allanguimaraes@id.uff.br. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2775, pp. 307-328. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Cryptococcus neoformans*/immunology , Antibodies, Monoclonal*/immunology , Antibodies, Monoclonal*/isolation & purification , Hybridomas*/immunology , Antibodies, Fungal*/immunology , Antibodies, Fungal*/isolation & purification, Animals ; Humans ; Mice ; B-Lymphocytes/immunology ; Cryptococcosis/immunology ; Cryptococcosis/diagnosis ; Antigens, Fungal/immunology ; Immunization |
| مستخلص: | The importance of humoral immunity to fungal infections remains to be elucidated. In cryptococcosis, patients that fail to generate antibodies against antigens of the fungus Cryptococcus neoformans are more susceptible to the disease, demonstrating the importance of these molecules to the antifungal immune response. Historically, antibodies against C. neoformans have been applied in diagnosis, therapeutics, and as important research tools to elucidate fungal biology. Throughout the process of generating monoclonal antibodies (mAbs) from a single B-cell clone and targeting a single epitope, several immunization steps might be required for the detection of responsive antibodies to the antigen of interest in the serum. This complex mixture of antibodies comprises the polyclonal antibodies. To obtain mAbs, B-lymphocytes are harvested (from spleen or peripheral blood) and fused with tumor myeloma cells, to generate hybridomas that are individually cloned and specifically screened for mAb production. In this chapter, we describe all the necessary steps, from the immunization to polyclonal antibody harvesting, hybridoma generation, and mAb production and purification. Additionally, we discuss new cutting-edge approaches for generating interspecies mAbs, such as humanized mAbs, or for similar species in distinct host backgrounds. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Antibodies; Capsule; Cryptococcus neoformans; Melanin; Monoclonal antibodiesAntibodies; Polyclonal antibodiesAntibodies |
| تواريخ الأحداث: | Date Created: 20240517 Date Completed: 20240517 Latest Revision: 20240628 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-0716-3722-7_20 |
| PMID: | 38758326 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-3722-7_20 |