دورية أكاديمية
أعرض في EDS

Mutations in the SARS-CoV-2 spike proteins affected the ACE2-binding affinity during the development of Omicron pandemic variants.

التفاصيل البيبلوغرافية
العنوان: Mutations in the SARS-CoV-2 spike proteins affected the ACE2-binding affinity during the development of Omicron pandemic variants.
المؤلفون: Tachibana K; Tokai University School of Medicine, Department of Internal Medicine, Division of Hematology and Oncology, 143 Shimokasuya, Isehara, Kanagawa, Japan. Electronic address: kouichi-tachibana@aist.go.jp., Nakamura Y; Tokai University School of Medicine, Center for Regenerative Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan., Do TL; The University of Kitakyusyu, Department of Life and Environment Engineering, 1-1 Hibikino, Wakamatu, Kitakyusyu, Fukuoka, Japan., Kihara T; The University of Kitakyusyu, Department of Life and Environment Engineering, 1-1 Hibikino, Wakamatu, Kitakyusyu, Fukuoka, Japan., Kawada H; Tokai University School of Medicine, Department of Internal Medicine, Division of Hematology and Oncology, 143 Shimokasuya, Isehara, Kanagawa, Japan., Yamamoto N; Tokai University School of Medicine, Department of Microbiology, 143 Shimokasuya, Isehara, Kanagawa, Japan., Ando K; Tokai University School of Medicine, Department of Internal Medicine, Division of Hematology and Oncology, 143 Shimokasuya, Isehara, Kanagawa, Japan.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Jul 30; Vol. 719, pp. 150120. Date of Electronic Publication: 2024 May 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Angiotensin-Converting Enzyme 2*/metabolism , Angiotensin-Converting Enzyme 2*/genetics , Spike Glycoprotein, Coronavirus*/genetics , Spike Glycoprotein, Coronavirus*/metabolism , Spike Glycoprotein, Coronavirus*/chemistry , SARS-CoV-2*/genetics , SARS-CoV-2*/metabolism , Mutation* , Protein Binding* , COVID-19*/virology , COVID-19*/metabolism, Humans ; Animals ; Mice ; Pandemics
مستخلص: Mutations in SARS-CoV-2 caused multiple waves of pandemics. To identify the function of such mutations, we investigated the binding affinity of the S protein with its receptor, ACE2. Omicron BA.1 showed significantly lower binding affinity with human ACE2 than prototype SARS-CoV-2 and Alpha strain, indicating that pre-Omicron to Omicron transition was not mediated by increasing the ACE2-binding affinity. Meanwhile, the later Omicron variants, BA.5 and XBB.1.5, showed significantly higher ACE2-binding affinity, suggesting that the increased ACE2-binding could be involved in the variant transition within Omicron strains. Furthermore, Alpha and Omicron variants, but not prototype SARS-CoV-2, bound mouse ACE2, which lead to a hypothesis that early Omicron strains evolved from Alpha strain by acquiring multiple mutations in mice.
Competing Interests: Declaration of Competing interest All authors declare that there is no conflict of interest in this study.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: ACE2; Binding; Mouse; Omicron; SARS-CoV-2; Spike protein
المشرفين على المادة: EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
0 (Spike Glycoprotein, Coronavirus)
0 (spike protein, SARS-CoV-2)
EC 3.4.17.23 (ACE2 protein, human)
EC 3.4.17.23 (Ace2 protein, mouse)
SCR Organism: SARS-CoV-2 variants
تواريخ الأحداث: Date Created: 20240517 Date Completed: 20240603 Latest Revision: 20240611
رمز التحديث: 20240611
DOI: 10.1016/j.bbrc.2024.150120
PMID: 38759524
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2024.150120