دورية أكاديمية
Similar neurotoxin expression profiles of traditional Chinese scorpion medicine material between juvenile and adult Mesobuthus martensii scorpions revealed by multiple strategic proteomics.
| العنوان: | Similar neurotoxin expression profiles of traditional Chinese scorpion medicine material between juvenile and adult Mesobuthus martensii scorpions revealed by multiple strategic proteomics. |
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| المؤلفون: | Guo Y; College of Life Sciences, Wuhan University, Wuhan, 430072, China., Zhu W; College of Life Sciences, Wuhan University, Wuhan, 430072, China., Yuan P; College of Life Sciences, Wuhan University, Wuhan, 430072, China., Huang X; College of Life Sciences, Wuhan University, Wuhan, 430072, China., Lu S; College of Life Sciences, Wuhan University, Wuhan, 430072, China., Cao Z; College of Life Sciences, Wuhan University, Wuhan, 430072, China., Zhao X; College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: zhaoxiaolu@whu.edu.cn., Wu Y; College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: ylwu@whu.edu.cn. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2024 Oct 05; Vol. 332, pp. 118338. Date of Electronic Publication: 2024 May 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Scorpions* , Proteomics*/methods , Scorpion Venoms* , Neurotoxins* , Medicine, Chinese Traditional*, Animals ; Animals, Poisonous |
| مستخلص: | Ethnopharmacological Relevance: The Mesobuthus martensii scorpions, called as "Quanxie", are known Chinese medicinal material base on the "Combat poison with poison" strategy for more than one thousand years, and still widely used to treat various diseases according to the Pharmacopoeia of the People's Republic of China nowadays. Aim of Study: The study aims to investigate the similarity of scorpion neurotoxins at the protein level between the juvenile and adult Mesobuthus martensii scorpions as Chinese medicine materials. Materials and Methods: The second-, third- and fourth-instar, and adult Mesobuthus martensii scorpions were collected for the characterization of neurotoxin expression through multiple strategic proteomics, including undigested scorpion venom, endopeptidase-digested, and undigested scorpion telson extract for the sample analysis. Results: Based on the known 107 scorpion neurotoxins from the genomic and transcriptomic analysis of adult Mesobuthus martensii scorpions, the multiple strategic proteomics first revealed that neurotoxins exhibited more stability in telson extract than secreted venom. In the reported transcripts of scorpion neurotoxins, approximately 53%, 56%, 66% and 78% of neurotoxins were detected through undigested scorpion venom, the endopeptidase Arg-C-, Lys-C-digested telson extract, and undigested telson extract strategies, respectively. Nearly 79% of scorpion neurotoxins detected in third-instar Mesobuthus martensii scorpions represent the largest number of scorpion neurotoxins from proteomic analysis to date. Moreover, a total of 84% of scorpion neurotoxins were successfully identified at the protein level, and similar neurotoxin expression profiles in second-, third- and fourth-instar, and adult Mesobuthus martensii scorpions were first revealed by the multiple strategic proteomics. Conclusion: These findings for the first time demonstrate the similar neurotoxin expression profiles between the juvenile and adult Mesobuthus martensii scorpions as Chinese medicinal material, which would serve as a paradigm for further toxin analysis from different venomous animals. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Arg-C digestion; Lys-C digestion; Mesobuthus martensii scorpion; Neurotoxin expression profile; Scorpion telson extract; Scorpion venom |
| المشرفين على المادة: | 0 (Scorpion Venoms) 0 (Neurotoxins) |
| SCR Organism: | Mesobuthus martensii |
| تواريخ الأحداث: | Date Created: 20240517 Date Completed: 20240611 Latest Revision: 20240611 |
| رمز التحديث: | 20240612 |
| DOI: | 10.1016/j.jep.2024.118338 |
| PMID: | 38759762 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2024.118338 |