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Genomic insights unveil the plasmid transfer mechanism and epidemiology of hypervirulent Klebsiella pneumoniae in Vietnam.

التفاصيل البيبلوغرافية
العنوان: Genomic insights unveil the plasmid transfer mechanism and epidemiology of hypervirulent Klebsiella pneumoniae in Vietnam.
المؤلفون: Nguyen Q; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Nguyen YTP; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Ha TT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Tran DTN; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Voong PV; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Chau V; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Nguyen PLN; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam., Le NTQ; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam., Nguyen LPH; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam., Nguyen TTN; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Trinh TV; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Carrique-Mas JJ; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Baker S; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID) Department of Medicine, University of Cambridge, Cambridge, UK., Thwaites G; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Rabaa MA; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Choisy M; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Chung HT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Pham DT; Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. duypt@oucru.org.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK. duypt@oucru.org.
المصدر: Nature communications [Nat Commun] 2024 May 17; Vol. 15 (1), pp. 4187. Date of Electronic Publication: 2024 May 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Klebsiella pneumoniae*/genetics , Klebsiella pneumoniae*/pathogenicity , Klebsiella pneumoniae*/isolation & purification , Plasmids*/genetics , Klebsiella Infections*/epidemiology , Klebsiella Infections*/microbiology , Phylogeny* , Whole Genome Sequencing*, Vietnam/epidemiology ; Humans ; Virulence/genetics ; Adult ; Female ; Gene Transfer, Horizontal ; Male ; Genome, Bacterial ; Middle Aged ; Anti-Bacterial Agents/pharmacology ; Child ; Genomics ; Drug Resistance, Bacterial/genetics
مستخلص: Hypervirulent Klebsiella pneumoniae (hvKp) is a significant cause of severe invasive infections in Vietnam, yet data on its epidemiology, population structure and dynamics are scarce. We screened hvKp isolates from patients with bloodstream infections (BSIs) at a tertiary infectious diseases hospital in Vietnam and healthy individuals, followed by whole genome sequencing and plasmid analysis. Among 700 BSI-causing Kp strains, 100 (14.3%) were hvKp. Thirteen hvKp isolates were identified from 350 rectal swabs of healthy adults; none from 500 rectal swabs of healthy children. The hvKp isolates were genetically diverse, encompassing 17 sequence types (STs), predominantly ST23, ST86 and ST65. Among the 113 hvKp isolates, 14 (12.6%) carried at least one antimicrobial resistance (AMR) gene, largely mediated by IncFII, IncR, and IncA/C plasmids. Notably, the acquisition of AMR conjugative plasmids facilitated horizontal transfer of the non-conjugative virulence plasmid between K. pneumoniae strains. Phylogenetic analysis demonstrated hvKp isolates from BSIs and human carriage clustered together, suggesting a significant role of intestinal carriage in hvKp transmission. Enhanced surveillance is crucial to understand the factors driving intestinal carriage and hvKp transmission dynamics for informing preventive measures. Furthermore, we advocate the clinical use of our molecular assay for diagnosing hvKp infections to guide effective management.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 222983/Z/21/Z Wellcome Trust (Wellcome); 0010734 Oxford University | John Fell Fund, University of Oxford (John Fell OUP Research Fund)
تواريخ الأحداث: Date Created: 20240517 Date Completed: 20240517 Latest Revision: 20240520
رمز التحديث: 20240520
مُعرف محوري في PubMed: PMC11101633
DOI: 10.1038/s41467-024-48206-3
PMID: 38760381
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-48206-3