دورية أكاديمية
أعرض في EDS

Functional extracellular vesicles from SHEDs combined with gelatin methacryloyl promote the odontogenic differentiation of DPSCs for pulp regeneration.

التفاصيل البيبلوغرافية
العنوان: Functional extracellular vesicles from SHEDs combined with gelatin methacryloyl promote the odontogenic differentiation of DPSCs for pulp regeneration.
المؤلفون: Lu H; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Mu Q; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Ku W; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Zheng Y; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Yi P; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Lin L; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Li P; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Wang B; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Wu J; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China., Yu D; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China. yudsh@mail.sysu.edu.cn., Zhao W; Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China. zhaowei3@mail.sysu.edu.cn.
المصدر: Journal of nanobiotechnology [J Nanobiotechnology] 2024 May 17; Vol. 22 (1), pp. 265. Date of Electronic Publication: 2024 May 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Dental Pulp*/cytology , Extracellular Vesicles*/chemistry , Gelatin*/chemistry , Gelatin*/pharmacology , Cell Differentiation*/drug effects , Odontogenesis*/drug effects , Stem Cells*/drug effects , Stem Cells*/cytology , Stem Cells*/metabolism , Regeneration*/drug effects , Tooth, Deciduous*/cytology , Methacrylates*/chemistry , Methacrylates*/pharmacology, Humans ; Animals ; Mice ; Cell Proliferation/drug effects ; Mice, Nude ; Cells, Cultured ; Hydrogels/chemistry ; Hydrogels/pharmacology ; Cell Movement/drug effects
مستخلص: Background: Pulp regeneration is a novel approach for the treatment of immature permanent teeth with pulp necrosis. This technique includes the combination of stem cells, scaffolds, and growth factors. Recently, stem cell-derived extracellular vesicles (EVs) have emerged as a new methodology for pulp regeneration. Emerging evidence has proven that preconditioning is an effective scheme to modify EVs for better therapeutic potency. Meanwhile, proper scaffolding is of great significance to protect EVs from rapid clearance and destruction. This investigation aims to fabricate an injectable hydrogel loaded with EVs from pre-differentiated stem cells from human exfoliated deciduous teeth (SHEDs) and examine their effects on pulp regeneration.
Results: We successfully employed the odontogenic induction medium (OM) of SHEDs to generate functional EV (OM-EV). The OM-EV at a concentration of 20 µg/mL was demonstrated to promote the proliferation and migration of dental pulp stem cells (DPSCs). The results revealed that OM-EV has a better potential to promote odontogenic differentiation of DPSCs than common EVs (CM-EV) in vitro through Alizarin red phalloidin, alkaline phosphatase staining, and assessment of the expression of odontogenic-related markers. High-throughput sequencing suggests that the superior effects of OM-EV may be attributed to activation of the AMPK/mTOR pathway. Simultaneously, we prepared a photocrosslinkable gelatin methacryloyl (GelMA) to construct an OM-EV-encapsulated hydrogel. The hydrogel exhibited sustained release of OM-EV and good biocompatibility for DPSCs. The released OM-EV from the hydrogel could be internalized by DPSCs, thereby enhancing their survival and migration. In tooth root slices that were subcutaneously transplanted in nude mice, the OM-EV-encapsulated hydrogel was found to facilitate dentinogenesis. After 8 weeks, there was more formation of mineralized tissue, as well as higher levels of dentin sialophosphoprotein (DSPP) and dentin matrix protein-1 (DMP-1).
Conclusions: The effects of EV can be substantially enhanced by preconditioning of SHEDs. The functional EVs from SHEDs combined with GelMA are capable of effectively promoting dentinogenesis through upregulating the odontogenic differentiation of DPSCs, which provides a promising therapeutic approach for pulp regeneration.
(© 2024. The Author(s).)
References: ACS Nano. 2019 Aug 27;13(8):8694-8707. (PMID: 31390518)
Biomater Res. 2023 Sep 15;27(1):86. (PMID: 37715230)
Stem Cell Res Ther. 2020 Jul 17;11(1):296. (PMID: 32680564)
Biomater Sci. 2020 Jun 21;8(12):3430-3442. (PMID: 32406432)
J Extracell Vesicles. 2022 Sep;11(9):e12266. (PMID: 36124834)
Stem Cell Res Ther. 2023 Apr 11;14(1):76. (PMID: 37038220)
Stem Cell Res Ther. 2020 Mar 6;11(1):110. (PMID: 32143712)
Cell Prolif. 2022 Dec;55(12):e13324. (PMID: 36054692)
Int J Mol Med. 2020 Oct;46(4):1571-1581. (PMID: 32945363)
Polymers (Basel). 2022 Aug 16;14(16):. (PMID: 36015596)
Sci Transl Med. 2018 Aug 22;10(455):. (PMID: 30135248)
Biotechnol Adv. 2022 Oct;59:107983. (PMID: 35588952)
J Mol Histol. 2020 Aug;51(4):455-466. (PMID: 32656578)
Regen Ther. 2023 Sep 22;24:489-498. (PMID: 37767183)
Cell Prolif. 2021 Jul;54(7):e13074. (PMID: 34101281)
Stem Cells Int. 2020 May 27;2020:5816723. (PMID: 32565828)
Mol Med Rep. 2019 Nov;20(5):4558-4566. (PMID: 31702021)
Int J Mol Sci. 2019 Apr 04;20(7):. (PMID: 30987410)
Adv Healthc Mater. 2022 Nov;11(21):e2201384. (PMID: 36053562)
J Nanobiotechnology. 2023 Sep 16;21(1):332. (PMID: 37716974)
J Endod. 2018 May;44(5):751-758. (PMID: 29426641)
J Extracell Vesicles. 2017 Oct 12;6(1):1388731. (PMID: 29184625)
Int J Mol Sci. 2021 Jun 08;22(12):. (PMID: 34201385)
Mol Med. 2021 Apr 26;27(1):43. (PMID: 33902432)
Acta Pharm Sin B. 2022 Oct;12(10):3822-3842. (PMID: 36213541)
ACS Nano. 2019 Sep 24;13(9):10279-10293. (PMID: 31483606)
J Nanobiotechnology. 2021 Nov 17;19(1):368. (PMID: 34789267)
Gerontology. 2018;64(2):127-134. (PMID: 29190625)
Stem Cell Res Ther. 2020 Jun 10;11(1):227. (PMID: 32522250)
Bioact Mater. 2020 Jul 16;5(4):1113-1126. (PMID: 32743122)
Chem Soc Rev. 2017 Oct 16;46(20):6255-6275. (PMID: 28816316)
Curr Stem Cell Res Ther. 2016;12(1):71-79. (PMID: 27527527)
J Nanobiotechnology. 2023 Oct 18;21(1):380. (PMID: 37848990)
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3561-3571. (PMID: 33405738)
Pharmaceutics. 2023 Jan 14;15(1):. (PMID: 36678909)
Mol Cell. 2017 Jun 15;66(6):789-800. (PMID: 28622524)
Int J Nanomedicine. 2021 Nov 30;16:7831-7846. (PMID: 34876812)
J Tissue Eng. 2022 Mar 16;13:20417314221084095. (PMID: 35321254)
J Biomech Eng. 2016 Jun;138(6):061006. (PMID: 27093446)
Int J Oral Sci. 2019 Aug 19;11(3):23. (PMID: 31423011)
Adv Sci (Weinh). 2020 Aug 07;7(19):2001334. (PMID: 33042751)
Stem Cells Int. 2021 Feb 5;2021:8876745. (PMID: 33628273)
Stem Cell Res Ther. 2019 Jun 13;10(1):170. (PMID: 31196201)
Differentiation. 2020 Jan - Feb;111:1-11. (PMID: 31630077)
ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091. (PMID: 30118197)
Theranostics. 2023 Mar 27;13(6):2015-2039. (PMID: 37064871)
J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094)
Mol Biol Rep. 2023 Feb;50(2):1281-1291. (PMID: 36451000)
Biomaterials. 2016 Dec;111:103-115. (PMID: 27728810)
ACS Omega. 2023 Feb 01;8(6):5393-5405. (PMID: 36816699)
Theranostics. 2020 Apr 27;10(13):5914-5931. (PMID: 32483427)
J Extracell Vesicles. 2021 Jan;10(3):e12061. (PMID: 33532042)
Anal Chem. 2015 Sep 15;87(18):9225-33. (PMID: 26291637)
معلومات مُعتمدة: 81974146 National Natural Sciences Foundation of China; 2023A1515012554 Guangdong Natural Sciences Foundation; 2019015 Sun Yat-sen University Clinical Research 5010 Programme
فهرسة مساهمة: Keywords: Extracellular vesicles; Hydrogel; Odontogenic differentiation; Pulp regeneration; Stem cells from human exfoliated deciduous teeth
المشرفين على المادة: 0 (gelatin methacryloyl)
تواريخ الأحداث: Date Created: 20240517 Date Completed: 20240518 Latest Revision: 20240521
رمز التحديث: 20240521
مُعرف محوري في PubMed: PMC11102175
DOI: 10.1186/s12951-024-02542-0
PMID: 38760763
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-3155
DOI:10.1186/s12951-024-02542-0