دورية أكاديمية
Orientin alleviates the inflammatory response in psoriasis like dermatitis in BALB/c mice by inhibiting the MAPK signaling pathway.
| العنوان: | Orientin alleviates the inflammatory response in psoriasis like dermatitis in BALB/c mice by inhibiting the MAPK signaling pathway. |
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| المؤلفون: | Long Q; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China; Molecular Biology Laboratory, Guizhou Medical University, Guiyang, Guizhou 550000, China., Ma T; Department of Dermatology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550000, China., Wang Y; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China., Chen S; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China; Department of Hepatobiliary Surgery, Guizhou Medical University Hospital, Guiyang, Guizhou 550000, China., Tang S; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China., Wang T; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China., Zhou Y; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China., Xu K; School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550000, China., Wan P; Department of Dermatology, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; Department of Dermatology, Guizhou Branch of Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Guiyang, Guizhou 550000, China. Electronic address: fof2007@126.com., Cao Y; Department of Dermatology, Guizhou Medical University Hospital, Guiyang, Guizhou 550000, China. Electronic address: caoyu@gmc.edu.cn. |
| المصدر: | International immunopharmacology [Int Immunopharmacol] 2024 Jun 15; Vol. 134, pp. 112261. Date of Electronic Publication: 2024 May 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Psoriasis*/drug therapy , Psoriasis*/immunology , Psoriasis*/chemically induced , Psoriasis*/pathology , Mice, Inbred BALB C* , Imiquimod* , Anti-Inflammatory Agents*/therapeutic use , Anti-Inflammatory Agents*/pharmacology , Flavonoids*/pharmacology , Flavonoids*/therapeutic use , Cytokines*/metabolism , HaCaT Cells* , Disease Models, Animal* , Lipopolysaccharides* , Keratinocytes*/drug effects , Glucosides*/therapeutic use , Glucosides*/pharmacology , MAP Kinase Signaling System*/drug effects, Animals ; Mice ; Humans ; RAW 264.7 Cells ; Skin/pathology ; Skin/drug effects ; Skin/immunology ; Cell Proliferation/drug effects ; Male ; Reactive Oxygen Species/metabolism ; Dermatitis/drug therapy ; Dermatitis/pathology ; Dermatitis/immunology ; Cell Line |
| مستخلص: | Background: Psoriasis, a chronic inflammatory condition of the skin, is characterized by an atypical proliferation of epidermal keratinocytes and immune cell infiltration. Orientin is a flavonoid monomer with potent anti-inflammatory activities. However, the therapeutic effects of orientin on psoriasis and the underlying mechanisms have not been elucidated. Objective: To investigate the therapeutic effect of orientin on psoriasis and the underlying mechanisms using network pharmacology and experimental studies. Methods: A psoriasis-like mouse model was established using imiquimod (IMQ). Lipopolysaccharide (LPS) was used to stimulate the RAW264.7 and HaCaT cells in vitro. The therapeutic effects of orientin and the underlying mechanism were analyzed using histopathological, immunohistochemical, quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, flow cytometry, and western blotting analyses. Results: Orientin ameliorated skin lesions and suppressed keratinocyte proliferation and immune cell infiltration in the IMQ-induced psoriasis-like mouse model. Additionally, orientin inhibited the secretion of the pro-inflammatory factors interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, IL-8, IL-17, and IL-23 in the psoriasis-like mouse model and LPS-induced RAW264.7 and HaCaT cells. Furthermore, orientin mitigated the LPS-induced upregulation of reactive oxygen species and downregulation of IL-10 and glutathione levels. Orientin alleviated inflammation by downregulating the MAPK signaling pathway. Conclusion: Orientin alleviated psoriasis-like dermatitis by suppressing the MAPK signaling pathway, suggesting that orientin is a potential therapeutic for psoriasis. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Inflammation; MAPK; Orientin; Oxidative Stress; Psoriasis |
| المشرفين على المادة: | P1QW714R7M (Imiquimod) 0 (Anti-Inflammatory Agents) 0 (Flavonoids) IAX93XCW6C (orientin) 0 (Cytokines) 0 (Lipopolysaccharides) 0 (Glucosides) 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20240518 Date Completed: 20240602 Latest Revision: 20240610 |
| رمز التحديث: | 20240610 |
| DOI: | 10.1016/j.intimp.2024.112261 |
| PMID: | 38761783 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1705 |
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| DOI: | 10.1016/j.intimp.2024.112261 |