دورية أكاديمية
High frequency of multidrug-resistant Escherichia coli from cattle in the Cerrado and Pantanal biomes of Brazil.
| العنوان: | High frequency of multidrug-resistant Escherichia coli from cattle in the Cerrado and Pantanal biomes of Brazil. |
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| المؤلفون: | Tutija JF; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: juliane_tutija@hotmail.com., Freitas MG; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: marigreenf@hotmail.com., Martinez EV; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: ericaverneque.martinez@gmail.com., Silva JFG; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: gjessicafernanda@gmail.com., Araripe MBM; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: araripemilena24@gmail.com., Leal CRB; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: cassia.leal@ufms.br., Souza Filho AF; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: antonio.filho@ufms.br., Ramos CAN; Federal University of Mato Grosso do Sul, Campo Grande, Brazil. Electronic address: carlos.nascimento@ufms.br. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2024 Jul; Vol. 192, pp. 106704. Date of Electronic Publication: 2024 May 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Drug Resistance, Multiple, Bacterial* , Escherichia coli Infections*/microbiology , Escherichia coli Infections*/veterinary , Escherichia coli Infections*/epidemiology , Feces*/microbiology , Anti-Bacterial Agents*/pharmacology , Escherichia coli*/drug effects , Escherichia coli*/genetics , Escherichia coli*/isolation & purification , Cattle Diseases*/microbiology , Cattle Diseases*/epidemiology , Virulence Factors*/genetics , Microbial Sensitivity Tests*, Animals ; Cattle ; Brazil ; Shiga-Toxigenic Escherichia coli/isolation & purification ; Shiga-Toxigenic Escherichia coli/genetics ; Shiga-Toxigenic Escherichia coli/drug effects ; Enterohemorrhagic Escherichia coli/genetics ; Enterohemorrhagic Escherichia coli/isolation & purification ; Enterohemorrhagic Escherichia coli/drug effects ; Enterotoxigenic Escherichia coli/drug effects ; Enterotoxigenic Escherichia coli/genetics ; Enterotoxigenic Escherichia coli/isolation & purification ; Escherichia coli Proteins/genetics |
| مستخلص: | The indiscriminate use of antimicrobials has led to the emergence of resistant bacteria, especially pathogenic strains of Escherichia coli, which are associated with diseases in animals and humans. The aim of the present study was to characterize E. coli isolates in calves with regards to the presence of virulence genes and investigate the resistance of the isolates to different antimicrobials. Between 2021 and 2023, 456 fecal samples were collected from calves in the Pantanal and Cerrado biomes of the state of Mato Grosso do Sul, Brazil. All samples were subjected to microbiological analysis and disc diffusion antibiogram testing. The polymerase chain reaction method was used to detect virulence genes. Bacterial growth was found in 451 of the 456 samples and biochemically identified as Escherichia coli. All 451 isolates (100 %) exhibited some phenotypic resistance to antimicrobials and 67.62 % exhibited multidrug resistance. The frequency of multidrug-resistant isolates in the Cerrado biome was significantly higher than that in the Pantanal biome (p = 0.0001). In the Cerrado, the most common pathotype was Shiga toxin-producing Escherichia coli (STEC) (28 %), followed by toxigenic Escherichia coli (ETEC) (11 %), enterohemorrhagic Escherichia coli (EHEC) (8 %) and enteropathogenic Escherichia coli (EPEC) (2 %). In most cases, the concomitant occurrence of pathotypes was more common, the most frequent of which were ETEC + STEC (33 %), ETEC + EHEC (15 %) and ETEC + EPEC (3 %). The STEC pathotype (30 %) was also found more frequently in the Pantanal, followed by EHEC (12 %), ETEC (9 %) and EPEC (6 %). The STEC pathotype had a significantly higher frequency of multidrug resistance (p = 0.0486) compared to the other pathotypes identified. The frequency of resistance was lower in strains from the Pantanal biome compared to those from the Cerrado biome. Although some factors are discussed in this paper, it is necessary to clarify the reasons for this difference and the possible impacts of these findings on both animal and human health in the region. Competing Interests: Declaration of competing interest I declare not to be presented with any type of conflict of interest between the participants or any other collaborator, direct or indirect, for the development of the Research Project published “Escherichia coli pathotypes in cattle: Distribution of antimicrobial-resistant strains between the Pantanal and Cerrado biomes in Brazil”, researchers involved are: Juliane Francielle Tutija, Mariana Green, Erica Verneque Martinez, Jessica Fernanda Gomes da Silva, Milena Batista Machado Araripe, Carlos Alberto do Nascimento Ramos, Cássia Rejane Brito Leal, Antônio Francisco de Souza Filho. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Colibacillosis; Diarrhea; Multidrug resistance; Virulence genes |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Virulence Factors) 0 (Escherichia coli Proteins) |
| تواريخ الأحداث: | Date Created: 20240518 Date Completed: 20240615 Latest Revision: 20240615 |
| رمز التحديث: | 20240616 |
| DOI: | 10.1016/j.micpath.2024.106704 |
| PMID: | 38761893 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2024.106704 |