دورية أكاديمية
Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
| العنوان: | Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models. |
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| المؤلفون: | Saraceni PR; Italian National Agency for New Technologies, Energy and Sustainable Development (ENEA), Department of Sustainability, 00123 Rome, Italy., Miccoli A; National Research Council, Institute for Marine Biological Resources and Biotechnology (IRBIM), 60125 Ancona, Italy., Bada A; Dept. for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Largo dell'Università snc, 01100 Viterbo, Italy., Taddei AR; Center of Large Equipments, Section of Electron Microscopy, University of Tuscia, Largo dell'Università Snc, 01100 Viterbo, Italy., Mazzonna M; National Research Council, Institute for Biological Systems (ISB), 00015 Monterotondo, Italy., Fausto AM; Dept. for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Largo dell'Università snc, 01100 Viterbo, Italy., Scapigliati G; Dept. for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Largo dell'Università snc, 01100 Viterbo, Italy., Picchietti S; Dept. for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Largo dell'Università snc, 01100 Viterbo, Italy. Electronic address: picchietti@unitus.it. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Jul 15; Vol. 934, pp. 173159. Date of Electronic Publication: 2024 May 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Water Pollutants, Chemical*/toxicity , Polystyrenes*/toxicity, Animals ; Fresh Water ; Transcriptome/drug effects ; Oncorhynchus mykiss/physiology ; Sea Bream/physiology ; Cell Line ; Ecotoxicology ; Seawater/chemistry ; Nanoparticles/toxicity |
| مستخلص: | The contamination of marine and freshwater environments by nanoplastics is considered a global threat for aquatic biota. Taking into account the most recent concentration range estimates reported globally and recognizing a knowledge gap in polystyrene nanoplastics (PS-NPs) ecotoxicology, the present work investigated the harmful effects of 20 nm and 80 nm PS-NPs, at increasing biological complexity, on the rainbow trout Oncorhynchus mykiss RTG-2 and gilthead seabream Sparus aurata SAF-1 cell lines. Twenty nm PS-NPs exerted a greater cytotoxicity than 80 nm ones and SAF-1 were approximately 4-fold more vulnerable to PS-NPs than RTG-2. The engagement of PS-NPs with plasma membranes was accompanied by discernible uptake patterns and morphological alterations along with a nuclear translocation already within a 30-min exposure. Cells were structurally damaged only by the 20 nm PS-NPs in a time-dependent manner as indicated by distinctive features of the execution phase of the apoptotic cell death mechanism such as cell shrinkage, plasma membrane blebbing, translocation of phosphatidylserine to the outer leaflet of the cell membrane and DNA fragmentation. At last, functional analyses unveiled marked transcriptional impairment at both sublethal and lethal doses of 20 nm PS-NPs, with the latter impacting the "Steroid biosynthesis", "TGF-beta signaling pathway", "ECM-receptor interaction", "Focal adhesion", "Regulation of actin cytoskeleton" and "Protein processing in endoplasmic reticulum" pathways. Overall, a distinct ecotoxicological hazard of PS-NPs at environmentally relevant concentrations was thoroughly characterized on two piscine cell lines. The effects were demonstrated to depend on size, exposure time and model, emphasizing the need for a comparative evaluation of endpoints between freshwater and marine ecosystems. Competing Interests: Declaration of competing interest The authors declare no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ATP; Ecotoxicology; Piscine cell lines; Polystyrene nanoplastics; RNAseq; Ultrastructure; Uptake and translocation |
| المشرفين على المادة: | 0 (Water Pollutants, Chemical) 0 (Polystyrenes) |
| تواريخ الأحداث: | Date Created: 20240518 Date Completed: 20240602 Latest Revision: 20240602 |
| رمز التحديث: | 20240602 |
| DOI: | 10.1016/j.scitotenv.2024.173159 |
| PMID: | 38761939 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2024.173159 |