دورية أكاديمية
أعرض في EDS

Pilot Study on Boiling Histotripsy Treatment of Human Leiomyoma Ex Vivo.

التفاصيل البيبلوغرافية
العنوان: Pilot Study on Boiling Histotripsy Treatment of Human Leiomyoma Ex Vivo.
المؤلفون: Ponomarchuk E; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia. Electronic address: ponomarchuk.em14@physics.msu.ru., Tsysar S; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Kvashennikova A; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Chupova D; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Pestova P; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia., Danilova N; Lomonosov Moscow State University, Medical Research and Educational Center, Moscow, Russia., Malkov P; Lomonosov Moscow State University, Medical Research and Educational Center, Moscow, Russia., Buravkov S; Lomonosov Moscow State University, Faculty of Fundamental Medicine, Moscow, Russia., Khokhlova V; Physics Faculty, Lomonosov Moscow State University, Moscow, Russia.
المصدر: Ultrasound in medicine & biology [Ultrasound Med Biol] 2024 Aug; Vol. 50 (8), pp. 1255-1261. Date of Electronic Publication: 2024 May 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 0410553 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-291X (Electronic) Linking ISSN: 03015629 NLM ISO Abbreviation: Ultrasound Med Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, New York, Pergamon Press.
مواضيع طبية MeSH: Leiomyoma*/therapy , Leiomyoma*/diagnostic imaging , Leiomyoma*/surgery , Uterine Neoplasms*/therapy , Uterine Neoplasms*/diagnostic imaging , High-Intensity Focused Ultrasound Ablation*/methods, Humans ; Female ; Pilot Projects ; Feasibility Studies ; In Vitro Techniques ; Treatment Outcome
مستخلص: Objective: As an alternative to surgical excision and magnetic resonance-guided thermal high-intensity focused ultrasound ablation of uterine leiomyoma, this work was aimed at pilot feasibility demonstration of use of ultrasound-guided boiling histotripsy for non-invasive non-thermal fractionation of human uterine leiomyoma ex vivo.
Methods: A custom-made sector ultrasound transducer of 1.5-MHz operating frequency and nominal f-number F# = 0.75 was used to produce a volumetric lesion (two layers of 5 × 5 foci with a 1 mm step) in surgically resected human leiomyoma ex vivo. A sequence of 10 ms pulses (P+/P-/A s = 157/-25/170 MPa in situ) with 1% duty cycle was delivered N = 30 times per focus under B-mode guidance. The treatment outcome was evaluated via B-mode imaging and histologically with hematoxylin and eosin and Masson's trichrome staining.
Results: The treatment was successfully performed in less than 30 min and resulted in formation of a rectangular lesion visualized on B-mode images during the sonication as an echogenic region, which sustained for about 10 min post-treatment. Histology revealed loss of cellular structure, necrotic debris and globules of degenerated collagen in the target volume surrounded by injured smooth muscle cells.
Conclusion: The pilot experiment described here indicates that boiling histotripsy is feasible for non-invasive mechanical disintegration of human uterine leiomyoma ex vivo under B-mode guidance, encouraging further investigation and optimization of this potential clinical application of boiling histotripsy.
Competing Interests: Conflict of interest The authors declare no competing interests.
(Copyright © 2024 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Boiling histotripsy; High-intensity focused ultrasound; Non-invasive surgery; Uterine leiomyoma
تواريخ الأحداث: Date Created: 20240518 Date Completed: 20240620 Latest Revision: 20240620
رمز التحديث: 20240621
DOI: 10.1016/j.ultrasmedbio.2024.05.002
PMID: 38762389
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-291X
DOI:10.1016/j.ultrasmedbio.2024.05.002