دورية أكاديمية
The α-linkage in funoran and agarose could be hydrolyzed by a GH96 family enzyme: Discovery of the α-funoranase.
| العنوان: | The α-linkage in funoran and agarose could be hydrolyzed by a GH96 family enzyme: Discovery of the α-funoranase. |
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| المؤلفون: | Zhang Y; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China., Zheng L; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China., Liu G; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China., Shen J; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China., Chen G; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China., Mei X; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China., Chang Y; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China; Qingdao Marine Science and Technology Center, 1 Wenhai Road, Qingdao 266237, China. Electronic address: changyg@ouc.edu.cn., Xue C; College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China; Qingdao Marine Science and Technology Center, 1 Wenhai Road, Qingdao 266237, China. |
| المصدر: | Carbohydrate polymers [Carbohydr Polym] 2024 Aug 15; Vol. 338, pp. 122201. Date of Electronic Publication: 2024 Apr 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1992-> : Barking : Elsevier Applied Science Publishers Original Publication: London [Eng.] : Applied Science Publishers, c1981- |
| مواضيع طبية MeSH: | Sepharose*/chemistry , Glycoside Hydrolases*/chemistry , Glycoside Hydrolases*/metabolism , Glycoside Hydrolases*/genetics, Hydrolysis ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Escherichia coli/genetics ; Galactans/chemistry ; Galactans/metabolism |
| مستخلص: | Agarans represent a group of galactans extracted from red algae. Funoran and agarose are the two major types and commercially applied polysaccharides of agaran. Although the glycoside hydrolases targeting β-glycosidic bonds of agaran have been widely investigated, those capable of degrading α-glycosidic bonds of agarose were limited, and the enzyme degrading α-linkages of funoran has not been reported till now. In this study, a GH96 family enzyme BiAF96A_Aq from a marine bacterium Aquimarina sp. AD1 was heterologously expressed in Escherichia coli. BiAF96A_Aq exhibited dual activities towards the characteristic structure of funoran and agarose, underscoring the multifunctionality of GH96 family members. Glycomics and NMR analysis revealed that BiAF96A_Aq hydrolyzed the α-1,3 glycosidic bonds between 3,6-anhydro-α-l-galactopyranose (LA) and β-d-galactopyranose-6-sulfate (G6S) of funoran, as well as LA and β-d-galactopyranose (G) of agarose, through an endo-acting manner. The end products of BiAF96A_Aq were majorly composed of disaccharides and tetrasaccharides. The identification of the activity of BiAF96A_Aq on funoran indicated the first discovery of the funoran hydrolase for α-1,3 linkage. Considering the novel catalytic reaction, we proposed to name this activity as "α-funoranase" and recommended the assignment of a dedicated EC number for its classification. Competing Interests: Declaration of competing interest The authors declare no conflict of interest. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Agarase; Agarose; Funoran; Funoranase; GH96; Hydrolysis pattern |
| تواريخ الأحداث: | Date Created: 20240519 Date Completed: 20240519 Latest Revision: 20240604 |
| رمز التحديث: | 20240605 |
| DOI: | 10.1016/j.carbpol.2024.122201 |
| PMID: | 38763726 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1344 |
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| DOI: | 10.1016/j.carbpol.2024.122201 |