Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores.
| العنوان: | Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores. |
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| المؤلفون: | Jradi FM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA., English BP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA., Brown TA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA., Aaron J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA., Khuon S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA., Galbraith JA; Department of Biomedical Engineering and Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA., Galbraith CG; Department of Biomedical Engineering and Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA., Lavis LD; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 May 12. Date of Electronic Publication: 2024 May 12. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Single-molecule localization microscopy (SMLM) uses activatable or switchable fluorophores to create non-diffraction limited maps of molecular location in biological samples. Despite the utility of this imaging technique, the portfolio of appropriate labels for SMLM remains limited. Here, we describe a general strategy for the construction of "glitter bomb" labels by simply combining rhodamine and coumarin dyes though an amide bond. Condensation of the ortho -carboxyl group on the pendant phenyl ring of rhodamine dyes with a 7-aminocoumarin yields photochromic or spontaneously blinking fluorophores depending on the parent rhodamine structure. We apply this strategy to prepare labels useful super-resolution experiments in fixed cells using different attachment techniques. This general glitter bomb strategy should lead to improved labels for SMLM, ultimately enabling the creation of detailed molecular maps in biological samples. |
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| معلومات مُعتمدة: | R01 GM117188 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: | Date Created: 20240520 Latest Revision: 20240531 |
| رمز التحديث: | 20240531 |
| مُعرف محوري في PubMed: | PMC11100827 |
| DOI: | 10.1101/2024.05.12.593749 |
| PMID: | 38766036 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2024.05.12.593749 |
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