أعرض في EDS

PCIF1 is partly cytoplasmic, dynamically localizes to stress granules and binds mRNA coding regions upon oxidative stress.

التفاصيل البيبلوغرافية
العنوان: PCIF1 is partly cytoplasmic, dynamically localizes to stress granules and binds mRNA coding regions upon oxidative stress.
المؤلفون: Tat TT; Center for RNA Therapeutics, Baylor College of Medicine, Houston TX.; Department of Cardiovascular Sciences, Baylor College of Medicine, Houston TX.; Houston Methodist Academic Institute, Baylor College of Medicine, Houston TX.; Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030 USA., Raza S; Technology Operations, Baylor College of Medicine, Houston TX.; Houston Methodist Academic Institute, Baylor College of Medicine, Houston TX.; Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030 USA., Khan S; Center for RNA Therapeutics, Baylor College of Medicine, Houston TX.; Department of Cardiovascular Sciences, Baylor College of Medicine, Houston TX.; Houston Methodist Academic Institute, Baylor College of Medicine, Houston TX.; Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030 USA., Watson TL; Center for RNA Therapeutics, Baylor College of Medicine, Houston TX.; Department of Cardiovascular Sciences, Baylor College of Medicine, Houston TX.; Houston Methodist Academic Institute, Baylor College of Medicine, Houston TX.; Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030 USA., Jung SY; Department of Molecular and Cellular Pharmacology, Baylor College of Medicine, Houston TX., Kiss DL; Center for RNA Therapeutics, Baylor College of Medicine, Houston TX.; Department of Cardiovascular Sciences, Baylor College of Medicine, Houston TX.; Houston Methodist Academic Institute, Baylor College of Medicine, Houston TX.; Weil Cornell Medical College, 6670 Bertner Ave, Houston, TX 77030 USA.; Houston Methodist Cancer Center, 6670 Bertner Ave, Houston, TX 77030 USA.; Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030 USA.
المصدر: BioRxiv : the preprint server for biology [bioRxiv] 2024 May 12. Date of Electronic Publication: 2024 May 12.
نوع المنشور: Preprint
اللغة: English
بيانات الدورية: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
مستخلص: PCIF1 ( P hosphorylated C TD- I nteracting F actor 1 ) is the mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase that catalyzes the formation of cap-adjacent N 6 ,2'-O-dimethyladenosine (m6Am) by methylating adenosines at the first transcribed position of capped mRNAs. While previous studies assumed that PCIF1 was nuclear, cell fractionation and immunofluorescence both show that a population of PCIF1 is localized to the cytoplasm. Further, PCIF1 redistributes to stress granules upon oxidative stress. Immunoprecipitation studies with stressed cells show that PCIF1 also physically interacts with G3BP and other stress granule components. In addition, PCIF1 behaves as a stress granule component as it disassociates from stress granules upon recovery from stress. Overexpressing full-length PCIF1 also inhibits stress granule formation, while knocking out PCIF1 slows stress granule disassembly. Next, our enhanced crosslinking and immunoprecipitation (eCLIP) data show that PCIF1 binds mRNAs in their coding sequences rather than cap-proximal regions. Further PCIF1's association with mRNAs increased upon NaAsO 2 stress. In contrast to eCLIP data, ChIP-Seq experiments show that PCIF1 is predominantly associated with transcription start sites rather than gene bodies, indicating that PCIF1's association with mature mRNA is not co-transcriptional. Collectively, our data suggest that PCIF1 has cytoplasmic RNA surveillance role(s) independent of transcription-associated cap-adjacent mRNA modification, particularly during the stress response.
References: Nucleic Acids Res. 2016 Jan 8;44(1):304-14. (PMID: 26673707)
Proc Natl Acad Sci U S A. 2021 Jul 20;118(29):. (PMID: 34266951)
Commun Biol. 2023 Sep 14;6(1):942. (PMID: 37709832)
Cell. 2016 Jan 28;164(3):487-98. (PMID: 26777405)
Dev Cell. 2023 Sep 11;58(17):1562-1577.e8. (PMID: 37625403)
Trends Biotechnol. 2021 Jan;39(1):72-89. (PMID: 32620324)
J Mol Biol. 2024 Mar 15;436(6):168496. (PMID: 38365086)
BMC Res Notes. 2010 Nov 10;3:294. (PMID: 21067583)
Biochem Biophys Res Commun. 2003 Feb 7;301(2):378-85. (PMID: 12565871)
Trends Cell Biol. 2016 Sep;26(9):668-679. (PMID: 27289443)
Mol Cell Proteomics. 2018 Nov;17(11):2270-2283. (PMID: 30093420)
Cell Res. 2019 Jan;29(1):80-82. (PMID: 30487554)
Mol Cell. 2020 Dec 3;80(5):876-891.e6. (PMID: 33217318)
Mol Cell. 2016 Dec 1;64(5):967-981. (PMID: 27912097)
Nat Cell Biol. 2019 Feb;21(2):162-168. (PMID: 30664789)
Biochem Biophys Res Commun. 2008 May 2;369(2):449-55. (PMID: 18294453)
Nature. 2023 Oct;622(7981):41-47. (PMID: 37794265)
Cell Rep. 2020 Aug 18;32(7):108038. (PMID: 32814042)
Nucleic Acids Res. 2021 Nov 8;49(19):10895-10910. (PMID: 34634806)
Cell. 2018 Jan 25;172(3):590-604.e13. (PMID: 29373831)
J Genet Genomics. 2014 Jan 20;41(1):21-33. (PMID: 24480744)
J Cell Biol. 2024 Jun 3;223(6):. (PMID: 38526325)
J Cell Biol. 2019 Dec 2;218(12):4127-4140. (PMID: 31636118)
J Mol Biol. 2020 Mar 27;432(7):2349-2368. (PMID: 32105731)
J Biol Chem. 2022 Apr;298(4):101751. (PMID: 35189146)
Mol Cell. 2019 Aug 8;75(3):631-643.e8. (PMID: 31279658)
Mol Cell. 2019 Aug 8;75(3):620-630.e9. (PMID: 31279659)
Nat Methods. 2021 Jul;18(7):713. (PMID: 34239096)
Mol Oncol. 2023 Dec;17(12):2526-2545. (PMID: 37149844)
Biochim Biophys Acta Mol Cell Res. 2021 Jan;1868(1):118876. (PMID: 33007331)
Nature. 2017 Jan 19;541(7637):371-375. (PMID: 28002401)
Nat Commun. 2021 Sep 20;12(1):5543. (PMID: 34545078)
J Cell Biol. 2020 Aug 3;219(8):. (PMID: 32520986)
EMBO J. 2022 May 2;41(9):e110137. (PMID: 35355287)
Biochemistry. 2022 May 23;:. (PMID: 35605980)
Nature. 1975 Sep 18;257(5523):251-3. (PMID: 1161029)
Nat Commun. 2023 Nov 15;14(1):7390. (PMID: 37968266)
FEBS Lett. 2019 Apr;593(7):670-679. (PMID: 30810230)
Science. 2019 Jan 11;363(6423):. (PMID: 30467178)
J Biol Chem. 2021 Jan-Jun;296:100270. (PMID: 33428944)
Nat Commun. 2021 Mar 19;12(1):1716. (PMID: 33741917)
Trends Biochem Sci. 2008 Mar;33(3):141-50. (PMID: 18291657)
Anal Chem. 2003 Feb 1;75(3):663-70. (PMID: 12585499)
J Biol Chem. 1978 Jul 25;253(14):5033-9. (PMID: 670176)
Methods. 2017 Aug 15;126:29-37. (PMID: 28790018)
Nucleic Acids Res. 1978 Mar;5(3):771-6. (PMID: 205837)
معلومات مُعتمدة: R35 GM137819 United States GM NIGMS NIH HHS
تواريخ الأحداث: Date Created: 20240520 Latest Revision: 20240531
رمز التحديث: 20240531
مُعرف محوري في PubMed: PMC11100685
DOI: 10.1101/2024.05.08.593175
PMID: 38766247
قاعدة البيانات: MEDLINE
الوصف
DOI:10.1101/2024.05.08.593175