دورية أكاديمية
أعرض في EDS

Inhibition of miR-199b-5p reduces pathological alterations in osteoarthritis by potentially targeting Fzd6 and Gcnt2 .

التفاصيل البيبلوغرافية
العنوان: Inhibition of miR-199b-5p reduces pathological alterations in osteoarthritis by potentially targeting Fzd6 and Gcnt2 .
المؤلفون: Feng T; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Zhang Q; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.; Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China., Li SH; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Ping YL; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Tian MQ; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Zhou SH; Department of Orthopedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, United States.; Harvard Stem Cell Institute, Harvard University, Cambridge, United States., Wang X; Departments of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, United States., Wang JM; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Liang FR; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China., Yu SG; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.; Key Laboratory of Acupuncture for Senile Disease (Chengdu University of TCM), Ministry of Education, Chengdu, China., Wu QF; Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.; Key Laboratory of Acupuncture for Senile Disease (Chengdu University of TCM), Ministry of Education, Chengdu, China.
المصدر: ELife [Elife] 2024 May 21; Vol. 12. Date of Electronic Publication: 2024 May 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: MicroRNAs*/genetics , MicroRNAs*/metabolism , Osteoarthritis*/genetics , Osteoarthritis*/pathology , Osteoarthritis*/metabolism , Frizzled Receptors*/genetics , Frizzled Receptors*/metabolism, Animals ; Mice ; Humans ; Male ; Mice, Inbred C57BL ; Chondrocytes/metabolism ; Disease Models, Animal ; Gene Expression Regulation
مستخلص: Osteoarthritis (OA) is a degenerative disease with a high prevalence in the elderly population, but our understanding of its mechanisms remains incomplete. Analysis of serum exosomal small RNA sequencing data from clinical patients and gene expression data from OA patient serum and cartilage obtained from the GEO database revealed a common dysregulated miRNA, miR-199b-5p. In vitro cell experiments demonstrated that miR-199b-5p inhibits chondrocyte vitality and promotes extracellular matrix degradation. Conversely, inhibition of miR-199b-5p under inflammatory conditions exhibited protective effects against damage. Local viral injection of miR-199b-5p into mice induced a decrease in pain threshold and OA-like changes. In an OA model, inhibition of miR-199b-5p alleviated the pathological progression of OA. Furthermore, bioinformatics analysis and experimental validation identified Gcnt2 and Fzd6 as potential target genes of MiR-199b-5p . Thus, these results indicated that MiR-199b-5p / Gcnt2 and Fzd6 axis might be a novel therapeutic target for the treatment of OA.
Competing Interests: TF, QZ, SL, YP, MT, SZ, XW, JW, FL, SY, QW No competing interests declared
(© 2023, Feng, Zhang et al.)
References: Br J Cancer. 2021 Mar;124(5):982-994. (PMID: 33239676)
Ann Rheum Dis. 2019 Jan;78(1):111-121. (PMID: 30287418)
Bio Protoc. 2017 Aug 20;7(16):. (PMID: 28920069)
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S17-23. (PMID: 20864019)
Nat Commun. 2021 Jul 6;12(1):4148. (PMID: 34230481)
Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438)
Arthritis Res Ther. 2014 Apr 01;16(2):R84. (PMID: 24690414)
Nat Commun. 2019 Jun 28;10(1):2876. (PMID: 31253842)
Nucleic Acids Res. 2019 Jan 8;47(D1):D155-D162. (PMID: 30423142)
Arthritis Res Ther. 2009;11(5):R133. (PMID: 19723327)
Nucleic Acids Res. 2022 Jul 5;50(W1):W216-W221. (PMID: 35325185)
Nucleic Acids Res. 2018 Jan 4;46(D1):D239-D245. (PMID: 29156006)
J Cell Mol Med. 2017 Jun;21(6):1159-1170. (PMID: 27957826)
Lancet. 2019 Apr 27;393(10182):1745-1759. (PMID: 31034380)
Bone Res. 2017 Jan 17;5:16044. (PMID: 28149655)
Nucleic Acids Res. 2024 Jan 5;52(D1):D1082-D1088. (PMID: 37953330)
J Vis Exp. 2016 May 16;(111):. (PMID: 27214709)
J Biomed Inform. 2011 Oct;44(5):839-47. (PMID: 21605702)
Nat Rev Rheumatol. 2021 Nov;17(11):692-705. (PMID: 34588660)
Environ Toxicol. 2022 Mar;37(3):593-602. (PMID: 34850997)
J Biol Chem. 2012 Jun 22;287(26):22206-15. (PMID: 22547066)
Nucleic Acids Res. 2014 Jan;42(Database issue):D92-7. (PMID: 24297251)
Science. 2020 Feb 7;367(6478):. (PMID: 32029601)
Nature. 2019 Feb;566(7743):254-258. (PMID: 30728500)
Mol Genet Genomics. 2023 Mar;298(2):343-352. (PMID: 36513842)
Arthritis Rheum. 2009 Sep;60(9):2723-30. (PMID: 19714579)
Cell Biochem Funct. 2019 Jan;37(1):42-51. (PMID: 30575058)
Int J Biochem Cell Biol. 2008;40(3):471-83. (PMID: 17931955)
Exp Gerontol. 2023 Apr;174:112103. (PMID: 36716981)
J Cell Biochem. 2019 Jan;120(1):171-181. (PMID: 30277597)
Biochem Biophys Res Commun. 2016 Sep 2;477(4):749-754. (PMID: 27363340)
J Exp Clin Cancer Res. 2016 Sep 29;35(1):156. (PMID: 27686593)
Nucleic Acids Res. 2020 Jan 8;48(D1):D127-D131. (PMID: 31504780)
J Tissue Eng Regen Med. 2020 Nov;14(11):1618-1629. (PMID: 32870569)
Elife. 2015 Aug 12;4:. (PMID: 26267216)
معلومات مُعتمدة: 2019YFC1709000 National Key Research and Development Program of China; 2022YFC3500703 National Key Research and Development Program of China; 2022ZDZX0033 Fund of Science and Technology Department of Sichuan Province; QNTD2022003 Xinglin Scholar Foundation of Chengdu University of Traditional Chinese Medicine
فهرسة مساهمة: Keywords: animal; extracellular matrix; immunology; inflammation; miRNA-199b-5p; mouse; osteoarthritis
سلسلة جزيئية: GEO GSE263996; GSE105027; GSE175961; GSE55235; GSE55457; GSE16464
المشرفين على المادة: 0 (FZD6 protein, human)
0 (mirn199 microRNA, human)
0 (Fzd6 protein, mouse)
0 (Mirn199 microRNA, mouse)
تواريخ الأحداث: Date Created: 20240521 Date Completed: 20240521 Latest Revision: 20240523
رمز التحديث: 20240523
مُعرف محوري في PubMed: PMC11108644
DOI: 10.7554/eLife.92645
PMID: 38770735
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.92645