دورية أكاديمية
أعرض في EDS

Multimodal inhibitory effect of matcha on Porphyromonas gingivalis .

التفاصيل البيبلوغرافية
العنوان: Multimodal inhibitory effect of matcha on Porphyromonas gingivalis .
المؤلفون: Nakao R; Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan., Takatsuka A; Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan.; Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon University, Kanagawa, Japan., Mandokoro K; Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon University, Kanagawa, Japan., Narisawa N; Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon University, Kanagawa, Japan., Ikeda T; Department of Pharmaceutical Sciences, Sojo University, Kumamoto, Japan., Takai H; Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan., Ogata Y; Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
المصدر: Microbiology spectrum [Microbiol Spectr] 2024 Jul 02; Vol. 12 (7), pp. e0342623. Date of Electronic Publication: 2024 May 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : ASM Press, 2013-
مواضيع طبية MeSH: Porphyromonas gingivalis*/drug effects , Porphyromonas gingivalis*/growth & development , Porphyromonas gingivalis*/physiology , Periodontitis*/microbiology , Anti-Bacterial Agents*/pharmacology , Bacterial Adhesion*/drug effects, Humans ; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Catechin/pharmacology ; Fusobacterium nucleatum/drug effects ; Fusobacterium nucleatum/growth & development ; Fusobacterium nucleatum/physiology ; Adult ; Prevotella nigrescens/drug effects ; Prevotella nigrescens/physiology ; Female ; Bacteroidaceae Infections/microbiology ; Male ; Aggregatibacter actinomycetemcomitans/drug effects ; Aggregatibacter actinomycetemcomitans/growth & development ; Aggregatibacter actinomycetemcomitans/physiology
مستخلص: Porphyromonas gingivalis has been associated with progression of periodontitis, characterized by inflammation and destruction of periodontal tissues. Here, we report that matcha, a product of Camellia sinensis , hampers the adherence and survival of P. gingivalis through multiple tactics. Matcha extract (ME) inhibited the growth not only of P. gingivalis but also of Prevotella nigrescen s and Fusobacterium nucleatum , while it did not inhibit growth of nine species of oral streptococci and Aggregatibacter actinomycetemcomitans . ME-mediated P. gingivalis growth inhibition was characterized by both morphological and physiological changes at the bacterial envelope, which were accompanied by nano-particle formation and decreased membrane fluidity/permeability without loss of membrane integrity. ME also triggered autoaggregation of P. gingivalis in a major fimbriae (FimA)-dependent manner. In addition, adherence of P. gingivalis was dramatically inhibited by ME, irrespective of fimbriae. Furthermore, a structure-activity relationship study tested a series of catechins isolated from ME and identified the pyrogallol-type B-ring of catechins as essential for P. gingivalis growth inhibition. In a clinical study to assess the microbiological and therapeutic effects of matcha mouthwash in patients with periodontitis, the P. gingivalis number in saliva was significantly reduced by matcha mouthwash compared to the pre-intervention level. A tendency toward improvement in probing pocket depth was observed in the matcha group, although the difference was not statistically significant. Taken together, we present a proof of concept, based on the multimodal inhibitory effect of matcha against P. gingivalis , and that matcha may have clinical applicability for prevention and treatment of periodontitis.
Importance: Periodontitis, a multifactorial inflammatory disease of the oral cavity, results in alveolar bone destruction, and is a major cause of tooth loss of humans. In addition, emerging evidence has demonstrated associations between periodontitis and a wide range of other chronic inflammation-driven disorders, including diabetes mellitus, preterm birth, cardiovascular disease, aspiration pneumonia, rheumatoid arthritis, cognitive disorder, and cancer. In the present study, we report that matcha, a product of Camellia sinensis , hampers Porphyromonas gingivalis , a major periodontal pathobiont, in not only a series of in vitro experiments but also a pilot intervention clinical trial of patients with periodontitis, in which matcha mouthwash statistically significantly reduced the P. gingivalis number in saliva, as compared to the pre-intervention level. Taken together, we suggest that matcha may have clinical applicability for prevention and treatment of periodontitis.
Competing Interests: The authors declare no conflict of interest.
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معلومات مُعتمدة: 90006400, 90009000 Matcha and Health Research with Kyoto Prefecture; 20H03861 MEXT KAKENHI; 21K18284, 21KK0164, 20H03861, 19K22644 JSPS KAKENHI
فهرسة مساهمة: Keywords: Camellia sinensis; FimA; Porphyromonas gingivalis; catechins; matcha; membrane fluidity; membrane potential; mouthwash; periodontal disease/periodontitis; probing pocket depth
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Plant Extracts)
8R1V1STN48 (Catechin)
تواريخ الأحداث: Date Created: 20240521 Date Completed: 20240702 Latest Revision: 20240711
رمز التحديث: 20240711
مُعرف محوري في PubMed: PMC11218439
DOI: 10.1128/spectrum.03426-23
PMID: 38771061
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-0497
DOI:10.1128/spectrum.03426-23