دورية أكاديمية
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Phosphatidic acid-dependent recruitment of microtubule motors to spherical supported lipid bilayers for in vitro motility assays.

التفاصيل البيبلوغرافية
العنوان: Phosphatidic acid-dependent recruitment of microtubule motors to spherical supported lipid bilayers for in vitro motility assays.
المؤلفون: Kumar P; Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India., Chaudhury D; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India., Sanghavi P; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India., Meghna A; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India., Mallik R; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India. Electronic address: rmallik@iitb.ac.in.
المصدر: Cell reports [Cell Rep] 2024 Jun 25; Vol. 43 (6), pp. 114252. Date of Electronic Publication: 2024 May 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
مواضيع طبية MeSH: Lipid Bilayers*/metabolism , Phosphatidic Acids*/metabolism , Phosphatidic Acids*/chemistry , Microtubules*/metabolism , Dictyostelium*/metabolism, Animals ; Rats ; Kinesins/metabolism ; Dyneins/metabolism
مستخلص: Motor proteins transport diverse membrane-bound vesicles along microtubules inside cells. How specific lipids, particularly rare lipids, on the membrane recruit and activate motors is poorly understood. To address this, we prepare spherical supported lipid bilayers (SSLBs) consisting of a latex bead enclosed within a membrane of desired lipid composition. SSLBs containing phosphatidic acid recruit dynein when incubated with Dictyostelium fractions but kinesin-1 when incubated with rat brain fractions. These SSLBs allow controlled biophysical investigation of membrane-bound motors along with their regulators at the single-cargo level in vitro. Optical trapping of single SSLBs reveals that motor-specific inhibitors can "lock" a motor to a microtubule, explaining the paradoxical arrest of overall cargo transport by such inhibitors. Increasing their size causes SSLBs to reverse direction more frequently, relevant to how large cargoes may navigate inside cells. These studies are relevant to understand how unidirectional or bidirectional motion of vesicles might be generated.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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فهرسة مساهمة: Keywords: CP: Molecular biology
المشرفين على المادة: 0 (Lipid Bilayers)
0 (Phosphatidic Acids)
EC 3.6.4.4 (Kinesins)
EC 3.6.4.2 (Dyneins)
تواريخ الأحداث: Date Created: 20240521 Date Completed: 20240701 Latest Revision: 20240705
رمز التحديث: 20240705
مُعرف محوري في PubMed: PMC11220796
DOI: 10.1016/j.celrep.2024.114252
PMID: 38771696
قاعدة البيانات: MEDLINE
الوصف
تدمد:2211-1247
DOI:10.1016/j.celrep.2024.114252