دورية أكاديمية
A new Zymomonas mobilis platform strain for the efficient production of chemicals.
| العنوان: | A new Zymomonas mobilis platform strain for the efficient production of chemicals. |
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| المؤلفون: | Frohwitter J; Analysis and Redesign of Biological Networks, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany., Behrendt G; Analysis and Redesign of Biological Networks, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany., Klamt S; Analysis and Redesign of Biological Networks, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany., Bettenbrock K; Analysis and Redesign of Biological Networks, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany. bettenbrock@mpi-magdeburg.mpg.de. |
| المصدر: | Microbial cell factories [Microb Cell Fact] 2024 May 22; Vol. 23 (1), pp. 143. Date of Electronic Publication: 2024 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101139812 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2859 (Electronic) Linking ISSN: 14752859 NLM ISO Abbreviation: Microb Cell Fact Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2002- |
| مواضيع طبية MeSH: | Zymomonas*/metabolism , Zymomonas*/genetics , Pyruvate Decarboxylase*/metabolism , Pyruvate Decarboxylase*/genetics , Metabolic Engineering*/methods , Ethanol*/metabolism , Lactic Acid*/metabolism , Lactic Acid*/biosynthesis , Escherichia coli*/metabolism , Escherichia coli*/genetics, L-Lactate Dehydrogenase/metabolism ; L-Lactate Dehydrogenase/genetics ; Alanine/metabolism ; Pyruvic Acid/metabolism ; Fermentation |
| مستخلص: | Background: Zymomonas mobilis is well known for its outstanding ability to produce ethanol with both high specific productivity and with high yield close to the theoretical maximum. The key enzyme in the ethanol production pathway is the pyruvate decarboxylase (PDC) which is converting pyruvate to acetaldehyde. Since it is widely considered that its gene pdc is essential, metabolic engineering strategies aiming to produce other compounds derived from pyruvate need to find ways to reduce PDC activity. Results: Here, we present a new platform strain (sGB027) of Z. mobilis in which the native promoter of pdc was replaced with the IPTG-inducible P Conclusion: We demonstrated that our new platform strain can be an excellent starting point for the efficient production of various compounds derived from pyruvate with Z. mobilis and can thus enhance the establishment of this organism as a workhorse for biotechnological production processes. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Zymomonas mobilis; Alanine; Lactate; Metabolic engineering; Platform strain; Pyruvate decarboxylase |
| تواريخ الأحداث: | Date Created: 20240521 Date Completed: 20240522 Latest Revision: 20240524 |
| رمز التحديث: | 20240524 |
| مُعرف محوري في PubMed: | PMC11110354 |
| DOI: | 10.1186/s12934-024-02419-9 |
| PMID: | 38773442 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1475-2859 |
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| DOI: | 10.1186/s12934-024-02419-9 |