دورية أكاديمية
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Single-Cell Patch-Clamp/Proteomics of Human Alzheimer's Disease iPSC-Derived Excitatory Neurons Versus Isogenic Wild-Type Controls Suggests Novel Causation and Therapeutic Targets.

التفاصيل البيبلوغرافية
العنوان: Single-Cell Patch-Clamp/Proteomics of Human Alzheimer's Disease iPSC-Derived Excitatory Neurons Versus Isogenic Wild-Type Controls Suggests Novel Causation and Therapeutic Targets.
المؤلفون: Ghatak S; Neurodegeneration New Medicines Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Diedrich JK; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Talantova M; Neurodegeneration New Medicines Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Bhadra N; Quantitative Medicine and Systems Biology, The Translational Genomics Research Institute, Phoenix, AZ, 85004, USA., Scott H; Neurodegeneration New Medicines Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Sharma M; Neurodegeneration New Medicines Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Albertolle M; Neurodegeneration New Medicines Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Schork NJ; Quantitative Medicine and Systems Biology, The Translational Genomics Research Institute, Phoenix, AZ, 85004, USA., Yates JR 3rd; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA., Lipton SA; Neurodegeneration New Medicines Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA.; Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
المصدر: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Aug; Vol. 11 (29), pp. e2400545. Date of Electronic Publication: 2024 May 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim : WILEY-VCH, [2014]-
مواضيع طبية MeSH: Alzheimer Disease*/metabolism , Alzheimer Disease*/genetics , Induced Pluripotent Stem Cells*/metabolism , Proteomics*/methods , Neurons*/metabolism , Patch-Clamp Techniques*/methods, Humans ; Single-Cell Analysis/methods
مستخلص: Standard single-cell (sc) proteomics of disease states inferred from multicellular organs or organoids cannot currently be related to single-cell physiology. Here, a scPatch-Clamp/Proteomics platform is developed on single neurons generated from hiPSCs bearing an Alzheimer's disease (AD) genetic mutation and compares them to isogenic wild-type controls. This approach provides both current and voltage electrophysiological data plus detailed proteomics information on single-cells. With this new method, the authors are able to observe hyperelectrical activity in the AD hiPSC-neurons, similar to that observed in the human AD brain, and correlate it to ≈1400 proteins detected at the single neuron level. Using linear regression and mediation analyses to explore the relationship between the abundance of individual proteins and the neuron's mutational and electrophysiological status, this approach yields new information on therapeutic targets in excitatory neurons not attainable by traditional methods. This combined patch-proteomics technique creates a new proteogenetic-therapeutic strategy to correlate genotypic alterations to physiology with protein expression in single-cells.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
References: Front Cell Neurosci. 2021 Sep 06;15:732429. (PMID: 34552470)
Nat Metab. 2020 Sep;2(9):873-881. (PMID: 32719536)
Transl Neurodegener. 2022 Jan 28;11(1):4. (PMID: 35090576)
Nat Methods. 2021 Jun;18(6):604-617. (PMID: 34099939)
Neurobiol Aging. 2021 Sep;105:99-114. (PMID: 34052751)
Ann Neurol. 1991 Oct;30(4):572-80. (PMID: 1789684)
Nat Med. 2020 May;26(5):769-780. (PMID: 32284590)
Front Neurosci. 2019 Feb 28;13:164. (PMID: 30872998)
Mol Neurodegener. 2020 Jan 10;15(1):4. (PMID: 31924226)
Front Chem. 2022 Apr 08;10:863979. (PMID: 35464213)
Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):E2518-27. (PMID: 23776240)
Arch Neurol. 2009 Apr;66(4):435-40. (PMID: 19204149)
Mol Cell Proteomics. 2020 Nov;19(11):1739-1748. (PMID: 32847821)
Adv Sci (Weinh). 2024 Mar;11(12):e2306469. (PMID: 38235614)
Nat Neurosci. 2022 Feb;25(2):213-225. (PMID: 35115731)
Genes (Basel). 2023 Jan 29;14(2):. (PMID: 36833274)
Aging (Albany NY). 2021 Jun 8;13(12):15750-15769. (PMID: 34102611)
Aging Cell. 2023 Sep;22(9):e13910. (PMID: 37357988)
PLoS One. 2009;4(1):e4183. (PMID: 19159013)
Brief Bioinform. 2018 Jan 1;19(1):1-11. (PMID: 27694351)
Lancet. 1998 Jul 25;352(9124):287. (PMID: 9690413)
Blood. 2020 Aug 6;136(6):726-739. (PMID: 32374849)
Cell Chem Biol. 2023 Aug 17;30(8):965-975.e6. (PMID: 37478858)
Methods Enzymol. 2019;628:263-292. (PMID: 31668233)
Adv Sci (Weinh). 2024 Aug;11(29):e2400545. (PMID: 38773714)
Cell Metab. 2022 May 3;34(5):654-655. (PMID: 35508106)
Cell Stem Cell. 2020 Sep 3;27(3):361-365. (PMID: 32888425)
Cell Genom. 2022 Sep 14;2(9):. (PMID: 36268052)
Cancer Cell Int. 2021 Oct 9;21(1):521. (PMID: 34627255)
Front Aging Neurosci. 2022 Nov 03;14:1003721. (PMID: 36408110)
Sci Rep. 2019 Dec 6;9(1):18509. (PMID: 31811163)
Cell. 2023 Sep 28;186(20):4438-4453.e23. (PMID: 37774681)
Front Synaptic Neurosci. 2020 Jul 23;12:28. (PMID: 32848694)
Mol Psychiatry. 2021 Oct;26(10):5751-5765. (PMID: 32467645)
Nature. 2016 May 05;533(7601):125-9. (PMID: 27120160)
Front Neurosci. 2021 Sep 21;15:734001. (PMID: 34621153)
Am J Physiol Cell Physiol. 2021 Jan 1;320(1):C106-C118. (PMID: 33112643)
Cell Rep. 2013 Nov 27;5(4):974-85. (PMID: 24239350)
Cell. 2023 Sep 28;186(20):4365-4385.e27. (PMID: 37774677)
Nat Med. 2017 Jun;23(6):678-680. (PMID: 28459436)
Mol Neurodegener. 2021 Aug 12;16(1):55. (PMID: 34384464)
Stem Cell Reports. 2023 Jul 11;18(7):1516-1533. (PMID: 37352850)
Nat Neurosci. 2018 Apr;21(4):463-473. (PMID: 29403035)
Anal Chem. 2022 Jan 25;94(3):1637-1644. (PMID: 34964611)
Sci Rep. 2016 Sep 08;6:33047. (PMID: 27605042)
Cell Mol Life Sci. 2023 May 7;80(6):141. (PMID: 37149819)
Nat Rev Neurosci. 2016 Dec;17(12):777-792. (PMID: 27829687)
Elife. 2019 Nov 29;8:. (PMID: 31782729)
Science. 2020 Jan 31;367(6477):512-513. (PMID: 32001644)
Annu Rev Neurosci. 2009;32:149-84. (PMID: 19555289)
Ann Neurol. 1990 May;27(5):457-64. (PMID: 2360787)
Genome Biol. 2021 Jan 27;22(1):50. (PMID: 33504367)
Cell. 2023 Sep 28;186(20):4257-4259. (PMID: 37774675)
Brain Commun. 2021 Oct 27;3(4):fcab261. (PMID: 34778762)
Neuron. 2018 Oct 24;100(2):330-348. (PMID: 30359600)
JAMA Neurol. 2013 Sep 1;70(9):1158-66. (PMID: 23835471)
Arch Gen Psychiatry. 2000 Jan;57(1):47-53. (PMID: 10632232)
Antioxidants (Basel). 2020 Nov 30;9(12):. (PMID: 33265993)
Int J Mol Sci. 2014 Nov 25;15(12):21740-53. (PMID: 25429433)
Mol Cell Pharmacol. 2010 Jan 1;2(2):43-46. (PMID: 20523917)
Anal Chem. 2023 Jun 20;95(24):9145-9150. (PMID: 37289937)
NIDA Res Monogr. 1994;139:127-53. (PMID: 8742555)
JAMA Psychiatry. 2020 Nov 1;77(11):1172-1180. (PMID: 32609320)
ACS Chem Neurosci. 2022 Jul 6;13(13):1992-2005. (PMID: 35758417)
Cell Metab. 2022 May 3;34(5):651-653. (PMID: 35508105)
Alzheimers Res Ther. 2020 Jul 6;12(1):80. (PMID: 32631408)
Front Aging Neurosci. 2020 Mar 12;12:38. (PMID: 32226377)
Cell. 2023 Sep 28;186(20):4404-4421.e20. (PMID: 37774679)
Front Neurosci. 2019 May 16;13:463. (PMID: 31156365)
Antioxidants (Basel). 2022 Oct 31;11(11):. (PMID: 36358532)
J Alzheimers Dis Rep. 2023 May 31;7(1):475-503. (PMID: 37313495)
Adv Drug Deliv Rev. 2020;159:214-231. (PMID: 31911096)
Elife. 2021 Jan 06;10:. (PMID: 33404500)
Nat Methods. 2023 Mar;20(3):320-323. (PMID: 36899157)
Cell. 2023 Sep 28;186(20):4422-4437.e21. (PMID: 37774680)
Struct Equ Modeling. 2020;27(6):975-984. (PMID: 33536726)
Hum Genomics. 2009 Jan;3(2):106-20. (PMID: 19164088)
F1000Res. 2020 Dec 10;9:1444. (PMID: 33604029)
Oncol Lett. 2022 Feb;23(2):58. (PMID: 34992690)
معلومات مُعتمدة: DP1DA041722 United States NH NIH HHS; R56 AG065372 United States AG NIA NIH HHS; F32ES031815 United States NH NIH HHS; R01 MH100175 United States MH NIMH NIH HHS; UH3 AG064706 United States AG NIA NIH HHS; F32 ES031815 United States ES NIEHS NIH HHS; DP1 DA041722 United States DA NIDA NIH HHS; R21 MH129776 United States MH NIMH NIH HHS; R21MH129776 United States NH NIH HHS; U19AG023122 United States NH NIH HHS; R35AG071734 United States NH NIH HHS; R01MH100175 United States NH NIH HHS; U24 AG078753 United States AG NIA NIH HHS; UH3AG064706 United States NH NIH HHS; R56AG065372 United States NH NIH HHS; R35 AG071734 United States AG NIA NIH HHS; U19 AG023122 United States AG NIA NIH HHS; R01 AG056259 United States AG NIA NIH HHS; U19AG065169 United States NH NIH HHS; R01 DA048882 United States DA NIDA NIH HHS; R01DA048882 United States NH NIH HHS; RF1 AG057409 United States AG NIA NIH HHS; U19 AG065169 United States AG NIA NIH HHS; U24AG078753 United States NH NIH HHS; RF1AG057409 United States NH NIH HHS; R01AG056259 United States NH NIH HHS
فهرسة مساهمة: Keywords: Alzheimer's disease; hiPSC‐derived neurons; patch clamp electrophysiology, single‐cell proteomics
تواريخ الأحداث: Date Created: 20240522 Date Completed: 20240807 Latest Revision: 20240809
رمز التحديث: 20240809
مُعرف محوري في PubMed: PMC11304297
DOI: 10.1002/advs.202400545
PMID: 38773714
قاعدة البيانات: MEDLINE
الوصف
تدمد:2198-3844
DOI:10.1002/advs.202400545