دورية أكاديمية
Ursoricin, a bacteriocin of Streptococcus ursoris , has potent activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci.
| العنوان: | Ursoricin, a bacteriocin of Streptococcus ursoris , has potent activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. |
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| المؤلفون: | Wang C; Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.; Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan., Le MN-T; Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan., Kawada-Matsuo M; Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan., Hisatsune J; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Sugawara Y; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Arai C; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Nakanishi J; Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan., Takeda K; Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan., Shiba H; Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan., Sugai M; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Komatsuzawa H; Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.; Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2024 Jun 18; Vol. 90 (6), pp. e0016224. Date of Electronic Publication: 2024 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacteriocins*/pharmacology , Bacteriocins*/genetics , Anti-Bacterial Agents*/pharmacology , Vancomycin-Resistant Enterococci*/drug effects , Methicillin-Resistant Staphylococcus aureus*/drug effects , Microbial Sensitivity Tests* , Biofilms*/drug effects , Streptococcus*/drug effects |
| مستخلص: | The emergence of drug-resistant bacteria, particularly methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), has increased the need to discover novel antimicrobial agents that are effective against these species. Here, we describe the identification and purification of the mutacin BHT-B-like gene locus and bacteriocin peptide from Streptococcus ursoris , which is closely related to Streptococcus ratti ; hence, we named this bacteriocin ursoricin. Ursoricin is a cationic, chromosome-encoded peptide that has potent antimicrobial effects against Gram-positive pathogens, including MRSA and VRE, with minimum inhibitory concentrations in the micromolar range. Ursoricin also inhibits the biofilm formation of high biofilm-forming S. aureus . Antibacterial activity was retained after treatment at 100°C for 60 min at a pH range of 3-9 and was partially reduced by treatment with proteinase K for 2 h (63% residual activity). The potent anti-MRSA, anti-VRE, and antibiofilm effects of ursoricin suggest that it is a possible candidate for the treatment of MRSA, VRE, and biofilm-associated infections. Importance: The emergence of multidrug-resistant bacteria worldwide has posed a significant public health threat and economic burdens that make the identification and development of novel antimicrobial agents urgent. Bacteriocins are promising new agents that exhibit antibacterial activity against a wide range of human pathogens. In this study, we report that the bacteriocin produced by Streptococcus ursoris showed good antibacterial activity against a wide range of Staphylococcus aureus and enterococcus strains, particularly methicillin-resistant Staphylococcus aureus , vancomycin-resistant enterococci, and high biofilm-forming S. aureus . Interestingly, this bacteriocin had a stronger effect on S. aureus than on Staphylococcus epidermidis , which is a major commensal bacterium in human skin; this result is important when considering the disturbance of bacterial flora, especially on the skin, mediated by the application of antibacterial agents. Competing Interests: The authors declare no conflict of interest. |
| معلومات مُعتمدة: | JP23fk0108606h0003 Japan Agency for Medical Research and Development (AMED) |
| فهرسة مساهمة: | Keywords: Streptococcus ursoris; anti-MRSA; anti-VRE; antimicrobial peptide; bacteriocin; ursoricin |
| المشرفين على المادة: | 0 (Bacteriocins) 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20240522 Date Completed: 20240618 Latest Revision: 20240618 |
| رمز التحديث: | 20240619 |
| DOI: | 10.1128/aem.00162-24 |
| PMID: | 38775468 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
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| DOI: | 10.1128/aem.00162-24 |