Symplasmic phloem loading and subcellular transport in storage roots are key factors for carbon allocation in cassava.

التفاصيل البيبلوغرافية
العنوان:	Symplasmic phloem loading and subcellular transport in storage roots are key factors for carbon allocation in cassava.
المؤلفون:	Rüscher D; Friedrich-Alexander-University Erlangen-Nuremberg, Department of Biology, Division of Biochemistry, Staudtstrasse 5, 91058 Erlangen, Germany., Vasina VV; School of Biological Sciences, Washington State University, Pullman, United States., Knoblauch J; School of Biological Sciences, Washington State University, Pullman, United States., Bellin L; University of Kaiserslautern-Landau (RPTU), Department of Biology, Division of Plant Physiology, Erwin-Schrödinger-Str. 22, 67663 Kaiserslautern., Pommerrenig B; University of Kaiserslautern-Landau (RPTU), Department of Biology, Division of Plant Physiology, Erwin-Schrödinger-Str. 22, 67663 Kaiserslautern., Alseekh S; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, 20 Germany., Fernie AR; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, 20 Germany., Neuhaus HE; University of Kaiserslautern-Landau (RPTU), Department of Biology, Division of Plant Physiology, Erwin-Schrödinger-Str. 22, 67663 Kaiserslautern., Knoblauch M; School of Biological Sciences, Washington State University, Pullman, United States., Sonnewald U; Friedrich-Alexander-University Erlangen-Nuremberg, Department of Biology, Division of Biochemistry, Staudtstrasse 5, 91058 Erlangen, Germany., Zierer W; Friedrich-Alexander-University Erlangen-Nuremberg, Department of Biology, Division of Biochemistry, Staudtstrasse 5, 91058 Erlangen, Germany.
المصدر:	Plant physiology [Plant Physiol] 2024 May 22. Date of Electronic Publication: 2024 May 22.
Publication Model:	Ahead of Print
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: American Society of Plant Biologists Country of Publication: United States NLM ID: 0401224 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2548 (Electronic) Linking ISSN: 00320889 NLM ISO Abbreviation: Plant Physiol Subsets: MEDLINE
أسماء مطبوعة:	Publication: : [Rockville, MD] : American Society of Plant Biologists Original Publication: Lancaster, Pa., American Society of Plant Physiologists.
مستخلص:	Cassava (Manihot esculenta) is a deciduous woody perennial shrub that stores large amounts of carbon and water in its storage roots. Previous studies have shown that assimilate unloading into storage roots happens symplasmically once secondary anatomy is established. However, mechanisms controlling phloem loading and overall carbon partitioning to different cassava tissues remain unclear. Here, we used a combination of histological, transcriptional, and biochemical analyses on different cassava tissues and at different timepoints to better understand source-sink carbon allocation. We found that cassava likely utilizes a predominantly passive symplasmic phloem loading strategy, indicated by the lack of expression of genes coding for key players of sucrose transport, the existence of branched plasmodesmata in the companion cell/bundle sheath interface of minor leaf veins, and very high leaf sucrose concentrations. Furthermore, we showed that tissue-specific changes in anatomy and non-structural carbohydrate (NSC) contents are associated with tissue-specific modification in gene expression for sucrose cleavage/synthesis, as well as subcellular compartmentalization of sugars. Overall, our data suggest that carbon allocation during storage root filling is mostly facilitated symplasmically and is likely mostly regulated by local tissue demand and subcellular compartmentalization. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
تواريخ الأحداث:	Date Created: 20240522 Latest Revision: 20240522
رمز التحديث:	20240522
DOI:	10.1093/plphys/kiae298
PMID:	38775728
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1532-2548
DOI:	10.1093/plphys/kiae298