دورية أكاديمية
أعرض في EDS

Aged garlic extract preserves beta-cell functioning via modulation of nuclear factor kappa-B (NF-κB)/Toll-like receptor (TLR)-4 and sarco endoplasmic reticulum calcium ATPase (SERCA)/Ca 2+ in diabetes mellitus.

التفاصيل البيبلوغرافية
العنوان: Aged garlic extract preserves beta-cell functioning via modulation of nuclear factor kappa-B (NF-κB)/Toll-like receptor (TLR)-4 and sarco endoplasmic reticulum calcium ATPase (SERCA)/Ca 2+ in diabetes mellitus.
المؤلفون: Ali SI; Preclinical Research Laboratory, Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India., Elkhalifa AME; Department of Public Health, College of Health Sciences, Saudi Electronic University, 11673, Riyadh, Saudi Arabia. a.alkhalifa@seu.edu.sa.; Department of Haematology, Faculty of Medical Laboratory Sciences, University of El Imam El Mahdi, Kosti, 1158, Sudan. a.alkhalifa@seu.edu.sa., Nabi SU; Preclinical Research Laboratory, Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India. showkatnabi@skuastkashmir.ac.in., Hayyat FS; Srinagar Women's College, Zakura, Srinagar, Jammu and Kashmir, 190024, India., Nazar M; Preclinical Research Laboratory, Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India., Taifa S; Preclinical Research Laboratory, Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India., Rakhshan R; Department of Clinical Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, 190006, India., Shah IH; Preclinical Research Laboratory, Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India., Shaheen M; Preclinical Research Laboratory, Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India., Wani IA; Department of Endocrinology and Clinical Research, Sher-I-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, 190002, India., Muzaffer U; Department of Medicine, Govt. Medical College, Srinagar, Jammu and Kashmir, India., Shah OS; Department of Sheep Husbandry, Srinagar, Jammu and Kashmir, 190006, India., Makhdoomi DM; Directorate of Extension, Sher-E-Kashmir University of Agricultural Sciences and Technology (SKUAST-Kashmir), Srinagar, Jammu and Kashmir, 190006, India., Ahmed EM; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 61922, Bisha, Saudi Arabia., Khalil KAA; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 61922, Bisha, Saudi Arabia., Bazie EA; Pediatric Department, Faculty of Medicine, University of El Imam El Mahdi, Kosti, 1158, Sudan., Zawbaee KI; Department of Blood Bank, Autonomous University of Barcelona, Al-Ghad International College for Applied Sciences, 155166, Riyadh, Saudi Arabia., Al Hasan Ali MM; Department of Pathology, Faculty of Medicine, Al-Baha University, Al-Baha, Saudi Arabia.; Department of Pathology, Faculty of Medicine, University of El Imam El Mahdi, Kosti, 1158, Sudan., Alanazi RJ; Department of Pharmacy Practice, College of Pharmacy, Alfaisal University, 50927, Riyadh, Saudi Arabia., Al Bataj IA; Ministry of Health, Najran General Hospital, 66262, Najran, Saudi Arabia., Al Gahtani SM; Department of Blood Bank, College of Applied Medical Sciences, University of King Saud, 11433, Riyadh, Saudi Arabia., Salwi AJ; Department of Blood Bank, College of Applied Medical Sciences, University of King Saud, 11433, Riyadh, Saudi Arabia., Alrodan LS; Department of Blood Bank, College of Applied Medical Sciences, University of King Saud, 11433, Riyadh, Saudi Arabia.
المصدر: Diabetology & metabolic syndrome [Diabetol Metab Syndr] 2024 May 22; Vol. 16 (1), pp. 110. Date of Electronic Publication: 2024 May 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101488958 Publication Model: Electronic Cited Medium: Print ISSN: 1758-5996 (Print) Linking ISSN: 17585996 NLM ISO Abbreviation: Diabetol Metab Syndr Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central
مستخلص: Background: Peripheral insulin resistance and compromised insulin secretion from pancreatic β-cells are significant factors and pathogenic hallmarks of diabetes mellitus (DM). NF-κβ/TLR-4 and SERCA/Ca 2+ pathways have been identified as potential pathways regulating insulin synthesis by preserving pancreatic β-cell functioning. The current study aimed to evaluate the therapeutic effect of aged garlic extract (AGE) against DM in a streptozotocin (STZ)-induced rat model with particular emphasis on pancreatic β-cell functioning.
Methods: AGE was characterized by gas chromatography-mass spectrometry (GC-MS), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) to evaluate its physio-chemical characteristics followed by in-vitro anti-diabetic and antioxidant potential. This was followed by the induction of DM in laboratory animals for investigating the therapeutic action of AGE by evaluating the role of NF-κβ/TLR-4 and the SERCA/Ca 2+ pathway. The parameters assessed in the present experimental setup encompassed antioxidant parameters, metabolic indicators, insulin concentration, intracellular calcium levels, apoptotic markers (CCK-8 and Caspase Glo-8), and protein expression (P-62 and APACHE-II).
Results: AGE characterization by SEM, GC-MS, and X-ray diffraction (XRD) revealed the presence of phenylalanine, alliin, S-allylmercaptocysteine (SAMC), tryptophan, 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid as major bioactive constituents of AGE. Metabolic studies, including intraperitoneal glucose tolerance test (IPGTT), revealed significantly lower blood glucose levels in the AGE group compared to the disease control group. In contrast, the intraperitoneal insulin tolerance test (ITT) exhibited no significant difference in insulin sensitivity between the AGE supplementation group and the DM control group. Interestingly, AGE was found to have no significant effect on fasting glucose and serum insulin levels. In contrast, AGE supplementation was found to cause significant hypoglycaemia in postprandial blood glucose and insulin levels. Importantly, AGE causes restoration of intracellular Ca 2+ levels by modulation of SERCA/Ca 2 functioning and inhibition NF-κB/TLR-4 pathway. AGE was found to interact with and inhibit the DR-5/ caspase-8/3 apoptotic complex. Furthermore, microscopic studies revealed degeneration and apoptotic changes in pancreatic β-cells of the DM control group, while supplementation of AGE resulted in inhibition of apoptotic pathway and regeneration of pancreatic β-cells.
Conclusion: The current study suggests that AGE enhance glucose homeostasis by exerting their effects on pancreatic β-cells, without ameliorating peripheral sensitivity. Moreover, AGEs promote an increase in β-cell mass by mitigating the apoptosis of pancreatic β-cells. These findings suggest that AGE could aid in developing a viable alternative therapy for diabetes mellitus (DM).
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Aged garlic extract; Diabetes mellitus; NF-κB/TLR-4; Pancreatic β-cells; SERCA/Ca2+; Streptozotocin
تواريخ الأحداث: Date Created: 20240522 Latest Revision: 20240525
رمز التحديث: 20240525
مُعرف محوري في PubMed: PMC11110209
DOI: 10.1186/s13098-024-01350-8
PMID: 38778421
قاعدة البيانات: MEDLINE
الوصف
تدمد:1758-5996
DOI:10.1186/s13098-024-01350-8