دورية أكاديمية
Viral load in hospitalized infants with respiratory syncytial virus bronchiolitis: a three-way comparative analysis.
العنوان: | Viral load in hospitalized infants with respiratory syncytial virus bronchiolitis: a three-way comparative analysis. |
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المؤلفون: | Golan-Tripto I; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel. Inbalgt@Clalit.org.il.; Pediatric Pulmonary Unit, Soroka University Medical Center, Beer Sheva, Israel. Inbalgt@Clalit.org.il.; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel. Inbalgt@Clalit.org.il., Danino D; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel.; Infectious Disease Unit, Soroka University Medical Center, Beer Sheva, Israel.; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel., De Waal L; Viroclinics DDL, Cerba Research Company, Rotterdam, The Netherlands., Akel K; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel., Dizitzer-Hillel Y; Department of Pediatrics 'C', Schneider's Children Medical Center of Israel, Petah Tikva, Israel., Tal A; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel., Langman Y; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel., Goldbart A; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel.; Pediatric Pulmonary Unit, Soroka University Medical Center, Beer Sheva, Israel.; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel. |
المصدر: | European journal of pediatrics [Eur J Pediatr] 2024 Aug; Vol. 183 (8), pp. 3471-3478. Date of Electronic Publication: 2024 May 23. |
نوع المنشور: | Journal Article; Comparative Study |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7603873 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1076 (Electronic) Linking ISSN: 03406199 NLM ISO Abbreviation: Eur J Pediatr Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Berlin : Springer Verlag Original Publication: Berlin, New York, Springer-Verlag. |
مواضيع طبية MeSH: | Viral Load* , Respiratory Syncytial Virus Infections*/diagnosis , Respiratory Syncytial Virus Infections*/virology , Bronchiolitis, Viral*/virology , Bronchiolitis, Viral*/diagnosis , Respiratory Syncytial Virus, Human*/isolation & purification , Real-Time Polymerase Chain Reaction*, Humans ; Infant ; Male ; Prospective Studies ; Female ; Specimen Handling/methods ; Hospitalization |
مستخلص: | Viral load measurement of Respiratory syncytial virus (RSV) in acute bronchiolitis depends on specimen collection, viral load quantification, and transport media. The aim of this study was to investigate viral load in three-way-comparative analyses; nasal swab versus nasal wash, quantitative real-time polymerase chain reaction (RT-PCR) versus cell tissue culture, and various transport media. A prospective cohort study of infants aged < 12 months, admitted to the Soroka Medical Center, due to acute bronchiolitis, was conducted. Two nasal swabs and two nasal wash samples (in UTM and VCM) were collected from each infant upon admission and after 48 h. Samples were immediately stored at -80 °C and tested at Viroclinics DDL (Rotterdam, Netherlands). Quantitative RT-PCR and quantitative virus culture were performed using tissue culture infective dose (TCID50). Spearman's correlation coefficient test assessed the correlation between the different methods, viral load, and clinical severity score. One hundred samples were collected from 13 infants (mean age 5.7 ± 3.8 months, 46% males). Twelve patients were RSV-A positive, and one was RSV-B positive. A high correlation was found between transport media- UTM and VCM (0.92, P < 0.001) and between nasal swabs and nasal wash samples (0.62, P = 0.02). RSV signals were higher in nasal wash than in swabs. PCR signals were lower in the second collection compared to the first. No correlation was found between viral load and clinical severity. Conclusion: RSV viral load is comparable across nasal wash, nasal swabs, and various transport media. However, it did not correlate with clinical severity, probably due to the limited sample size. Broader analyses are warranted. What is Known: • Viral load measurement in Respiratory Syncytial Virus (RSV) bronchiolitis depends on specimen collection, viral load quantification, and transport media. • The COVID-19 pandemic underscored the paramount significance of proper specimen collection, notably through nasal swabs. What is New: • RSV viral load was investigated in three-way-comparative analyses. • RSV viral load correlated well across PCR and tissue culture, nasal wash and swabs, and various transport media. RSV viral load did not correlate with clinical severity. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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فهرسة مساهمة: | Keywords: Bronchiolitis; Nasal swab; Nasal wash; Respiratory syncytial virus; Viral load |
تواريخ الأحداث: | Date Created: 20240523 Date Completed: 20240722 Latest Revision: 20240722 |
رمز التحديث: | 20240722 |
DOI: | 10.1007/s00431-024-05614-3 |
PMID: | 38780651 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1432-1076 |
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DOI: | 10.1007/s00431-024-05614-3 |