دورية أكاديمية
Viral load in hospitalized infants with respiratory syncytial virus bronchiolitis: a three-way comparative analysis.
| العنوان: | Viral load in hospitalized infants with respiratory syncytial virus bronchiolitis: a three-way comparative analysis. |
|---|---|
| المؤلفون: | Golan-Tripto I; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel. Inbalgt@Clalit.org.il.; Pediatric Pulmonary Unit, Soroka University Medical Center, Beer Sheva, Israel. Inbalgt@Clalit.org.il.; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel. Inbalgt@Clalit.org.il., Danino D; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel.; Infectious Disease Unit, Soroka University Medical Center, Beer Sheva, Israel.; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel., De Waal L; Viroclinics DDL, Cerba Research Company, Rotterdam, The Netherlands., Akel K; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel., Dizitzer-Hillel Y; Department of Pediatrics 'C', Schneider's Children Medical Center of Israel, Petah Tikva, Israel., Tal A; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel., Langman Y; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel., Goldbart A; Department of Pediatrics, Soroka University Medical Center, Beer Sheva, Israel.; Pediatric Pulmonary Unit, Soroka University Medical Center, Beer Sheva, Israel.; Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel. |
| المصدر: | European journal of pediatrics [Eur J Pediatr] 2024 Aug; Vol. 183 (8), pp. 3471-3478. Date of Electronic Publication: 2024 May 23. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7603873 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1076 (Electronic) Linking ISSN: 03406199 NLM ISO Abbreviation: Eur J Pediatr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Verlag Original Publication: Berlin, New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Viral Load* , Respiratory Syncytial Virus Infections*/diagnosis , Respiratory Syncytial Virus Infections*/virology , Bronchiolitis, Viral*/virology , Bronchiolitis, Viral*/diagnosis , Respiratory Syncytial Virus, Human*/isolation & purification , Real-Time Polymerase Chain Reaction*, Humans ; Infant ; Male ; Prospective Studies ; Female ; Specimen Handling/methods ; Hospitalization |
| مستخلص: | Viral load measurement of Respiratory syncytial virus (RSV) in acute bronchiolitis depends on specimen collection, viral load quantification, and transport media. The aim of this study was to investigate viral load in three-way-comparative analyses; nasal swab versus nasal wash, quantitative real-time polymerase chain reaction (RT-PCR) versus cell tissue culture, and various transport media. A prospective cohort study of infants aged < 12 months, admitted to the Soroka Medical Center, due to acute bronchiolitis, was conducted. Two nasal swabs and two nasal wash samples (in UTM and VCM) were collected from each infant upon admission and after 48 h. Samples were immediately stored at -80 °C and tested at Viroclinics DDL (Rotterdam, Netherlands). Quantitative RT-PCR and quantitative virus culture were performed using tissue culture infective dose (TCID50). Spearman's correlation coefficient test assessed the correlation between the different methods, viral load, and clinical severity score. One hundred samples were collected from 13 infants (mean age 5.7 ± 3.8 months, 46% males). Twelve patients were RSV-A positive, and one was RSV-B positive. A high correlation was found between transport media- UTM and VCM (0.92, P < 0.001) and between nasal swabs and nasal wash samples (0.62, P = 0.02). RSV signals were higher in nasal wash than in swabs. PCR signals were lower in the second collection compared to the first. No correlation was found between viral load and clinical severity. Conclusion: RSV viral load is comparable across nasal wash, nasal swabs, and various transport media. However, it did not correlate with clinical severity, probably due to the limited sample size. Broader analyses are warranted. What is Known: • Viral load measurement in Respiratory Syncytial Virus (RSV) bronchiolitis depends on specimen collection, viral load quantification, and transport media. • The COVID-19 pandemic underscored the paramount significance of proper specimen collection, notably through nasal swabs. What is New: • RSV viral load was investigated in three-way-comparative analyses. • RSV viral load correlated well across PCR and tissue culture, nasal wash and swabs, and various transport media. RSV viral load did not correlate with clinical severity. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| References: | Boyce TG, Mellen BG, Mitchel EF Jr, Wright PF, Griffin MR (2000) Rates of hospitalization for respiratory syncytial virus infection among children in medicaid. J Pediatr 137:865–870. (PMID: 10.1067/mpd.2000.11053111113845) Wang EE, Law BJ, Stephens D (1995) Pediatric investigators collaborative Ne2rk on infections in Canada (PICNIC) prospective study of risk factors and outcomes in patients hospitalized with respiratory syncytial viral lower respiratory tract infection. J Pediatr 126:212–219. (PMID: 10.1016/S0022-3476(95)70547-37844667) Hall CB, Weinberg GA, Iwane MK et al (2009) The burden of respiratory syncytial virus infection in young children. N Engl J Med 360:588–598. (PMID: 10.1056/NEJMoa0804877191966754829966) Nair H, Nokes DJ, Gessner BD et al (2010) Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. Lancet 375:1545–1555. (PMID: 10.1016/S0140-6736(10)60206-1203994932864404) Perkins SM, Webb DL, Torrance SA et al (2005) Comparison of a real-time reverse transcriptase PCR assay and a culture technique for quantitative assessment of viral load in children naturally infected with respiratory syncytial virus. J Clin Microbiol 43:2356–2362. (PMID: 10.1128/JCM.43.5.2356-2362.2005158722661153767) Lambert SB, Whiley DM, O’Neill NT et al Comparing nose-throat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction. Pediatrics 2008;122;e615. (PMID: 10.1542/peds.2008-069118725388) El Saleeby CM, Bush AJ, Harrison LM, Aitken JA, Devincenzo JP (2011) Respirator syncytial virus load, viral dynamics, and disease severity in previously healthy naturally infected children. J Infect Dis 204:996–1002. (PMID: 10.1093/infdis/jir494218811133203391) de Waal L, Smits SL, Veldhuis Kroeze EJB, van Amerongen G, Pohl MO, Osterhaus AD, M. E, Stittelaar KJ (2018) Transmission of human respiratory syncytial virus in the immunocompromised ferret model. Viruses 10(1):18. (PMID: 10.3390/v10010018293013135795431) Stittelaar KJ, de Waal L, van Amerongen G, Veldhuis Kroeze EJ, Fraaij PL, van Baalen CA, van Kampen JJ, van der Vries E, Osterhaus AD, de Swart RL (2016) Ferrets as a novel animal model for studying human respiratory syncytial virus infections in immunocompetent and immunocompromised hosts. Viruses 8(6):168. (PMID: 10.3390/v8060168273143794926188) Golan-Tripto I, Goldbart A, Akel KH, Dizitzer Y, Novack V, Tal A (2018) Modified Tal Score: validated score for prediction of bronchiolitis severity. Ped Pulmo 53(6):796–801. (PMID: 10.1002/ppul.24007) DeVincenzo JP et al (2014) Oral GS-5806 activity in a respiratory syncytial virus challenge study. N Engl J Med 371:711–722. (PMID: 10.1056/NEJMoa140118425140957) Jafri HS, Wu X, Makari D, Henrickson KJ (2013) Distribution of respiratory syncytial virus subtypes A and B among infants presenting to the emergency department with lower respiratory tract infection or apnea. Pediatr Infect Dis J 32(4):335–40. (PMID: 10.1097/INF.0b013e318282603a23337904) Thwaites RS, Ito K, Chingono JMS, Coates M, Jarvis HC, Tunstall T, Anderson-Dring L, Cass L, Rapeport G, Openshaw PJ, Nadel S, Hansel TT (2017) Nasosorption as a minimally invasive sampling procedure: mucosal viral load and inflammation in primary RSV bronchiolitis. J Infect Dis 215(8):1240–1244. (PMID: 10.1093/infdis/jix150283684905441107) Jochems SP, Piddock K, Rylance J et al (2017) Novel analysis of immune cells from nasal microbiopsy demonstrates reliable, reproducible data for immune populations, and superior cytokine detection compared to nasal wash. PLoS ONE 12:e0169805. (PMID: 10.1371/journal.pone.0169805281074575249128) Wright PF, Gruber WC, Peters M, Reed G, Zhu Y, Robinson F, Coleman-Dockery S, Graham BS (2002) Illness severity, viral shedding, and antibody responses in infants hospitalized with bronchiolitis caused by respiratory syncytial virus. J Infect Dis 185:1011–1018. (PMID: 10.1086/33982211930309) Garnett L, Bello A, Tran KN, Audet J, Leung A, Schiffman Z, Griffin BD, Tailor N, Kobasa D, Strong JE (2020) Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages. J Virol Methods 285:113947. (PMID: 10.1016/j.jviromet.2020.113947327810087414358) Bidkar V, Selvaraj K, Mishra M, Shete V, Sajjanar A (2021 Mar-Apr) A comparison of swab types on sample adequacy, suspects comfort and provider preference in COVID-19. Am J Otolaryngol 42(2):102872. (PMID: 10.1016/j.amjoto.2020.10287233418177) Legg JP, Hussain IR, Warner JA, Johnston SL, Warner JO (2003) Type 1 and type 2 cytokine imbalance in acute respiratory syncytial virus bronchiolitis. Am J Respir Crit Care Med 168:633–639. (PMID: 10.1164/rccm.200210-1148OC12773328) Buckingham S, Bush A, DeVincenzo J (2000) Nasal quantity of respiratory syncytial virus correlates with disease severity in hospitalized infants. Pediatr Infect Dis J 19:113–117. (PMID: 10.1097/00006454-200002000-0000610693996) Hasegawa K, Jartti T, Mansbach JM et al (2015) Respiratory syncytial virus genomic load and disease severity among children hospitalized with bronchiolitis: multicenter cohort studies in the United States and Finland. J Infect Dis 211:1550–1559. (PMID: 10.1093/infdis/jiu65825425699) DeVincenzo JP, El Saleeby CM, Bush AJ (2005) Respiratory syncytial virus load predicts disease severity in previously healthy infants. J Infect Dis 191:1861–1868. (PMID: 10.1086/43000815871119) Houben M, Coenjaerts F, Rossen J, Belderbos M, Hofland R, Kimpen J, Bont L (2010) Disease severity and viral load are correlated in infants with primary respiratory syncytial virus infection in the community. J Med Virol 82:1266–1271. (PMID: 10.1002/jmv.21771205130947167003) Flynn MF, Kelly M, Dooley JSG (2021) Nasopharyngeal swabs vs. nasal aspirates for respiratory virus detection: a systematic review. Pathogens 10(11):1515. (PMID: 10.3390/pathogens10111515348326708620365) Todsen T, Bohr A, Hovgaard LH, Eið RC, Benfield T, Svendsen MBS, Kirkby N, Konge L, von Buchwald C, Melchiors J, Tolsgaard M (2021) Valid and reliable assessment of upper respiratory. Valid and reliable assessment of upper respiratory tract specimen collection skills during the COVID-19 pandemic 11(11):1987. |
| فهرسة مساهمة: | Keywords: Bronchiolitis; Nasal swab; Nasal wash; Respiratory syncytial virus; Viral load |
| تواريخ الأحداث: | Date Created: 20240523 Date Completed: 20240722 Latest Revision: 20240722 |
| رمز التحديث: | 20240722 |
| DOI: | 10.1007/s00431-024-05614-3 |
| PMID: | 38780651 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-1076 |
|---|---|
| DOI: | 10.1007/s00431-024-05614-3 |