دورية أكاديمية
Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis.
| العنوان: | Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis. |
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| المؤلفون: | Maruri-Aransolo A; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain., de Dios Caballero J; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. juan.dcaballero@gmail.com.; CIBER de Enfermedades Infecciosas CIBERINFEC, Instituto de Salud Carlos III, Madrid, Spain. juan.dcaballero@gmail.com., Michelena M; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain., Medina-Pascual MJ; Laboratorio de Referencia e Investigación en Taxonomía, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain., Carrasco G; Laboratorio de Referencia e Investigación en Taxonomía, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain., Asensio O; Pediatric Pulmonology Unit, Consorci Corporació Sanitària Parc Taulí, Sabadell, Spain., Cols M; Pediatric Pulmonology Department and Cystic Fibrosis Unit, Hospital Sant Joan de Déu, Barcelona, Spain., Cantón R; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; CIBER de Enfermedades Infecciosas CIBERINFEC, Instituto de Salud Carlos III, Madrid, Spain. |
| مؤلفون مشاركون: | GEIFQ group |
| المصدر: | European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2024 Jul; Vol. 43 (7), pp. 1349-1353. Date of Electronic Publication: 2024 May 23. |
| نوع المنشور: | Journal Article; Evaluation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Original Publication: [Wiesbaden, Federal Republic of Germany] : Vieweg, [c1988- |
| مواضيع طبية MeSH: | Cystic Fibrosis*/microbiology , Cystic Fibrosis*/complications , Burkholderia cepacia complex*/isolation & purification , Burkholderia cepacia complex*/classification , Sputum*/microbiology , Burkholderia Infections*/microbiology , Burkholderia Infections*/diagnosis , Culture Media*/chemistry , Agar* , Sensitivity and Specificity* , Bacteriological Techniques*/methods, Humans |
| مستخلص: | Introduction: Burkholderia cepacia complex (BCC) are non-fermenting Gram-negative bacteria that can chronically colonize the lungs of people with cystic fibrosis (pwCF), causing a severe and progressive respiratory failure, post-transplant complications and epidemic outbreaks. Therefore, rapid and accurate identification of these bacteria is relevant for pwCF, in order to facilitate early eradication and prevent chronic colonization. However, BCCs are often quite difficult to detect on culture media as they have a slow growth rate and can be hidden by other fast-growing microorganisms, including Pseudomonas aeruginosa and filamentous fungi. Material and Methods: We evaluated the sensitivity of CHROMagar™ B. cepacia agar using 11 isolates from a well-characterized BCC collection, using BCA agar (Oxoid, UK) as a gold standard. We also studied 180 clinical sputum samples to calculate positive (PPV) and negative (NPV) predictive values. Furthermore, we used three of the well-characterized BCC isolates to determine the limit of detection (LOD). Results: Eleven isolates grew on CHROMagar™ B. cepacia at 37ºC after 48 h. The NPV and PPV of CHROMagar™ B. cepacia were 100% and 87.5%, respectively. The LOD of CHROMagar™ B. cepacia was around 1 × 10 3 CFU/ml, requiring a ten-fold dilution lower bacterial load than BCA for BCC detection. Conclusion: CHROMagar™ B. cepacia agar proved to have a very good sensitivity and specificity for the detection of clinical BCCs. Moreover, the chromogenic nature of the medium allowed us to clearly differentiate BCC from other Gram-negative species, filamentous fungi and yeasts, thereby facilitating the identification of contaminants. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | Project PI19/01043 Instituto de Salud Carlos III; JR18/00034 Instituto de Salud Carlos III; CB21/13/00084 Instituto de Salud Carlos III |
| فهرسة مساهمة: | Keywords: Burkholderia cepacia complex; CHROMagar™; Chronic respiratory infections; Cystic fibrosis |
| المشرفين على المادة: | 0 (Culture Media) 9002-18-0 (Agar) |
| تواريخ الأحداث: | Date Created: 20240523 Date Completed: 20240719 Latest Revision: 20240727 |
| رمز التحديث: | 20240727 |
| مُعرف محوري في PubMed: | PMC11271321 |
| DOI: | 10.1007/s10096-024-04845-4 |
| PMID: | 38780755 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1435-4373 |
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| DOI: | 10.1007/s10096-024-04845-4 |