دورية أكاديمية
Effects of clinical covariates on serum miRNA expression among women without ovarian cancer.
| العنوان: | Effects of clinical covariates on serum miRNA expression among women without ovarian cancer. |
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| المؤلفون: | Wollborn L; Brigham and Women's Hospital, Boston, MA, United States., Webber JW; Brigham and Women's Hospital, Boston, United States., Alimena S; Brigham and Women's Hospital, Boston, MA, United States., Mishra S; Brigham and Women's Hospital, Boston, MA, United States., Sussman CB; Harvard Medical School, Boston, United States., Comrie CE; Harvard Medical School, United States., Packard DG; Tufts University, Boston, MA, United States., Williams M; Harvard Medical School, Boston, United States., Russell T; Harvard Medical School, Boston, United States., Fendler W; Medical University of Lodz, Lodz, Poland., Chowdhury D; Dana Farber Cancer Institute, Harvard Medical School, Boston, United States., Elias KM; Brigham and Women's Hospital, Boston, MA, United States. |
| المصدر: | Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology [Cancer Epidemiol Biomarkers Prev] 2024 May 23. Date of Electronic Publication: 2024 May 23. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 9200608 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-7755 (Electronic) Linking ISSN: 10559965 NLM ISO Abbreviation: Cancer Epidemiol Biomarkers Prev Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Philadelphia, PA : American Association for Cancer Research, c1991- |
| مستخلص: | Background: Serum microRNAs (miRNAs) are potential biomarkers for ovarian cancer; however, many factors may influence miRNA expression. To understand potential confounders in miRNA analysis, we examined how sociodemographic factors and comorbidities, including known ovarian cancer risk factors, influence serum miRNA levels in women without ovarian cancer. Methods: Data from 1,576 women from the Mass General Brigham Biobank collected between 2012 and 2019, excluding subjects previously or subsequently diagnosed with ovarian cancer, were examined. Using a focused panel of 179 miRNA probes optimized for serum profiling, miRNA expression was measured by flow cytometry using the Abcam Fireplex® assay and correlated with subjects' electronic medical records. Results: The study population broadly reflected the New England population. The median age of subjects was 49 years, 34% were current or prior smokers, 33% were obese (BMI >30kg/m2), 49% were postmenopausal, and 11% had undergone prior bilateral oophorectomy. Significant differences in miRNA expression were observed among ovarian risk factors such as age, obesity, menopause, BRCA1 or BRCA2 germline mutations or breast cancer in family history. Additionally, miRNA expression was significantly altered by prior bilateral oophorectomy, hypertension, and hypercholesterolemia. Other variables, such as smoking, parity, age at menarche, hormonal replacement therapy, oral contraception, breast, endometrial, or colon cancer, and diabetes were not associated with significant changes in the panel when corrected for multiple testing. Conclusions: Serum miRNA expression patterns are significantly affected by patient demographics, exposure history, and medical comorbidities. Impact: Understanding confounders in serum miRNA expression is important for refining clinical assays for cancer screening. |
| تواريخ الأحداث: | Date Created: 20240523 Latest Revision: 20240523 |
| رمز التحديث: | 20240523 |
| DOI: | 10.1158/1055-9965.EPI-23-1355 |
| PMID: | 38780899 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1538-7755 |
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| DOI: | 10.1158/1055-9965.EPI-23-1355 |