دورية أكاديمية
أعرض في EDS

Neurexin drives Caenorhabditis elegans avoidance behavior independently of its post-synaptic binding partner neuroligin.

التفاصيل البيبلوغرافية
العنوان: Neurexin drives Caenorhabditis elegans avoidance behavior independently of its post-synaptic binding partner neuroligin.
المؤلفون: Muirhead CS; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01605, USA., Reddy KC; Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA., Guerra S; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01605, USA., Rieger M; Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA., Hart MP; Department of Genetics, University of Pennsylvania, Philadelphia, PA 19104, USA., Srinivasan J; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01605, USA., Chalasani SH; Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
المصدر: G3 (Bethesda, Md.) [G3 (Bethesda)] 2024 Aug 07; Vol. 14 (8).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Print Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Bethesda, MD : Genetics Society of America, 2011-
مواضيع طبية MeSH: Caenorhabditis elegans*/metabolism , Caenorhabditis elegans*/genetics , Caenorhabditis elegans*/physiology , Caenorhabditis elegans Proteins*/metabolism , Caenorhabditis elegans Proteins*/genetics , Cell Adhesion Molecules, Neuronal*/metabolism , Cell Adhesion Molecules, Neuronal*/genetics , Protein Isoforms*/metabolism, Animals ; Mutation ; Avoidance Learning ; Behavior, Animal ; Animals, Genetically Modified ; Glycerol/metabolism ; Neurons/metabolism ; Calcium-Binding Proteins/metabolism ; Calcium-Binding Proteins/genetics ; Synapses/metabolism ; Protein Binding ; Neuroligins
مستخلص: Neurexins and their canonical binding partners, neuroligins, are localized to neuronal pre-, and post-synapses, respectively, but less is known about their role in driving behaviors. Here, we use the nematode C. elegans to show that neurexin, but not neuroligin, is required for avoiding specific chemorepellents. We find that adults with knockouts of the entire neurexin locus exhibit a strong avoidance deficit in response to glycerol and a weaker defect in response to copper. Notably, the C. elegans neurexin (nrx-1) locus, like its mammalian homologs, encodes multiple isoforms, α and γ. Using isoform-specific mutations, we find that the γ isoform is selectively required for glycerol avoidance. Next, we used transgenic rescue experiments to show that this isoform functions at least partially in the nervous system. We also confirm that the transgenes are expressed in the neurons and observe protein accumulation in neurites. Furthermore, we tested whether these mutants affect the behavioral responses of juveniles. We find that juveniles (4th larval stages) of mutants knocking out the entire locus or the α-isoforms, but not γ-isoform, are defective in avoiding glycerol. These results suggest that the different neurexin isoforms affect chemosensory avoidance behavior in juveniles and adults, providing a general principle of how isoforms of this conserved gene affect behavior across species.
Competing Interests: Conflicts of interest The author(s) declare no conflicts of interest.
(© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America.)
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معلومات مُعتمدة: P40 OD010440 United States CD ODCDC CDC HHS; P40 OD010440 United States OD NIH HHS; Nippert Foundation; Caenorhabditis Genetics Center; R01 DC016058 United States DC NIDCD NIH HHS; R01 MH096881 United States MH NIMH NIH HHS; 1R35GM146782 United States GF NIH HHS
فهرسة مساهمة: Keywords: C elegans; avoidance assays; behavior; neurexin; neuroligin-independent
المشرفين على المادة: 0 (Caenorhabditis elegans Proteins)
0 (Cell Adhesion Molecules, Neuronal)
0 (Protein Isoforms)
0 (neuroligin 1)
0 (nrx-1 protein, C elegans)
PDC6A3C0OX (Glycerol)
0 (Calcium-Binding Proteins)
0 (Neuroligins)
تواريخ الأحداث: Date Created: 20240523 Date Completed: 20240807 Latest Revision: 20240809
رمز التحديث: 20240809
مُعرف محوري في PubMed: PMC11304965
DOI: 10.1093/g3journal/jkae111
PMID: 38781440
قاعدة البيانات: MEDLINE
الوصف
تدمد:2160-1836
DOI:10.1093/g3journal/jkae111