دورية أكاديمية
Characterization of a novel multifunctional β-glucosidase/xylanase/feruloyl esterase and its effects on improving the quality of Longjing tea.
| العنوان: | Characterization of a novel multifunctional β-glucosidase/xylanase/feruloyl esterase and its effects on improving the quality of Longjing tea. |
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| المؤلفون: | Wang H; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China; College of Life Engineering, Shenyang Institute of Technology, Fushun, China. Electronic address: 376097301@qq.com., Qi X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. Electronic address: qxh@ujs.edu.cn., Gao S; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China., Kan G; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China., Damdindorj L; Department of Biology, National University of Mongolia, Ulaanbaatar, Mongolia. Electronic address: lkhagvasuren.d@num.edu.mn., An Y; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China. Electronic address: anyingfeng666@163.com., Lu F; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin, China. Electronic address: lfp@tust.edu.cn. |
| المصدر: | Food chemistry [Food Chem] 2024 Sep 30; Vol. 453, pp. 139637. Date of Electronic Publication: 2024 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
| مواضيع طبية MeSH: | Carboxylic Ester Hydrolases*/chemistry , Carboxylic Ester Hydrolases*/metabolism , beta-Glucosidase*/chemistry , beta-Glucosidase*/metabolism , Camellia sinensis*/chemistry , Camellia sinensis*/enzymology , Tea*/chemistry, Endo-1,4-beta Xylanases/chemistry ; Endo-1,4-beta Xylanases/metabolism ; Odorants/analysis |
| مستخلص: | Herein, a novel multifunctional enzyme β-glucosidase/xylanase/feruloyl esterase (GXF) was constructed by fusion of β-glucosidase and bifunctional xylanase/feruloyl esterase. The activities of β-glucosidase, xylanase, feruloyl esterase and acetyl xylan esterase displayed by GXF were 67.18 %, 49.54 %, 38.92 % and 23.54 %, respectively, higher than that of the corresponding single functional enzymes. Moreover, the GXF performed better in enhancing aroma and quality of Longjing tea than the single functional enzymes and their mixtures. After treatment with GXF, the grassy and floral odors of tea infusion were significantly improved. Moreover, GXF treatment could improve concentrations of flavonoid aglycones of myricetin, kaempferol and quercetin by 68.1-, 81.42- and 77.39-fold, respectively. In addition, GXF could accelerate the release of reducing sugars, ferulic acid and xylo-oligosaccharides by 9.48-, 8.25- and 4.11-fold, respectively. This multifunctional enzyme may have potential applications in other fields such as food production and biomass degradation. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aroma; Flavonoid aglycone; Longjing tea; Multifunctional enzyme; β-glucosidase/xylanase/feruloyl esterase |
| المشرفين على المادة: | EC 3.1.1.73 (feruloyl esterase) EC 3.1.1.- (Carboxylic Ester Hydrolases) EC 3.2.1.21 (beta-Glucosidase) 0 (Tea) EC 3.2.1.8 (Endo-1,4-beta Xylanases) |
| تواريخ الأحداث: | Date Created: 20240523 Date Completed: 20240602 Latest Revision: 20240611 |
| رمز التحديث: | 20240611 |
| DOI: | 10.1016/j.foodchem.2024.139637 |
| PMID: | 38781897 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7072 |
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| DOI: | 10.1016/j.foodchem.2024.139637 |