دورية أكاديمية
Mobility of sodium ions in agarose gels probed through combined single- and triple-quantum NMR.
| العنوان: | Mobility of sodium ions in agarose gels probed through combined single- and triple-quantum NMR. |
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| المؤلفون: | Nimerovsky E; Department of NMR-based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11 D-37077 Göttingen, Germany., Sieme D; Department of NMR-based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11 D-37077 Göttingen, Germany., Rezaei-Ghaleh N; Heinrich Heine University Düsseldorf, Faculty of Mathematics and Natural Sciences, Institute of Physical Biology, Universitätsstraße 1 D-40225 Düsseldorf, Germany; Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich D-52428 Jülich, Germany. Electronic address: Nasrollah.Rezaie.Ghaleh@hhu.de. |
| المصدر: | Methods (San Diego, Calif.) [Methods] 2024 Aug; Vol. 228, pp. 55-64. Date of Electronic Publication: 2024 May 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Duluth, MN : Academic Press Original Publication: San Diego : Academic Press, c1990- |
| مواضيع طبية MeSH: | Sepharose*/chemistry , Sodium*/chemistry , Gels*/chemistry, Magnetic Resonance Spectroscopy/methods ; Ions/chemistry ; Quantum Theory |
| مستخلص: | Metal ions, including biologically prevalent sodium ions, can modulate electrostatic interactions frequently involved in the stability of condensed compartments in cells. Quantitative characterization of heterogeneous ion dynamics inside biomolecular condensates demands new experimental approaches. Here we develop a 23 Na NMR relaxation-based integrative approach to probe dynamics of sodium ions inside agarose gels as a model system. We exploit the electric quadrupole moment of spin-3/2 23 Na nuclei and, through combination of single-quantum and triple-quantum-filtered 23 Na NMR relaxation methods, disentangle the relaxation contribution of different populations of sodium ions inside gels. Three populations of sodium ions are identified: a population with bi-exponential relaxation representing ions within the slow motion regime and two populations with mono-exponential relaxation but at different rates. Our study demonstrates the dynamical heterogeneity of sodium ions inside agarose gels and presents a new experimental approach for monitoring dynamics of sodium and other spin-3/2 ions (e.g. chloride) in condensed environments. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Condensates; Counterions; Hydrogels; Phase separation; quadrupolar NMR |
| المشرفين على المادة: | 9012-36-6 (Sepharose) 9NEZ333N27 (Sodium) 0 (Gels) 0 (Ions) |
| تواريخ الأحداث: | Date Created: 20240523 Date Completed: 20240613 Latest Revision: 20240613 |
| رمز التحديث: | 20240614 |
| DOI: | 10.1016/j.ymeth.2024.05.015 |
| PMID: | 38782295 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9130 |
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| DOI: | 10.1016/j.ymeth.2024.05.015 |