دورية أكاديمية
أعرض في EDS

Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth.

التفاصيل البيبلوغرافية
العنوان: Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth.
المؤلفون: Yoon JY; Institute for Stem Cell & Matters, Cell & Matter Corporation, Cheonan 31116, Republic of Korea.; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Vu HT; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Pediatric Dentistry, Faculty of Odonto-Stomatology, University of Medincine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 17000, Vietnam.; Department of Pediatric Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Lee JH; Institute for Stem Cell & Matters, Cell & Matter Corporation, Cheonan 31116, Republic of Korea.; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Nanobiomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Mechanobiology Dental Medicine Research Center, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Cell & Matter Institute, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Shin JS; Department of Pediatric Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Kim HW; Institute for Stem Cell & Matters, Cell & Matter Corporation, Cheonan 31116, Republic of Korea.; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Nanobiomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Mechanobiology Dental Medicine Research Center, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Cell & Matter Institute, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Lee HH; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Nanobiomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Kim JB; Department of Pediatric Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea., Lee JH; Institute for Stem Cell & Matters, Cell & Matter Corporation, Cheonan 31116, Republic of Korea.; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Department of Nanobiomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Mechanobiology Dental Medicine Research Center, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.; Cell & Matter Institute, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.
المصدر: Cells [Cells] 2024 May 16; Vol. 13 (10). Date of Electronic Publication: 2024 May 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Tooth, Deciduous*/cytology , Stem Cells*/cytology , Stem Cells*/metabolism , Blood Platelets*/metabolism , Cell Differentiation*/drug effects , Cell Proliferation*/drug effects, Humans ; Cattle ; Animals ; Dental Pulp/cytology ; Cell Movement/drug effects ; Culture Media/pharmacology ; Cells, Cultured ; Cell Extracts/pharmacology ; Hydrogen Peroxide/pharmacology ; Oxidative Stress/drug effects ; Cell Survival/drug effects
مستخلص: In recent years, there has been a surge in demand for and research focus on cell therapy, driven by the tissue-regenerative and disease-treating potentials of stem cells. Among the candidates, dental pulp stem cells (DPSCs) or human exfoliated deciduous teeth (SHED) have garnered significant attention due to their easy accessibility (non-invasive), multi-lineage differentiation capability (especially neurogenesis), and low immunogenicity. Utilizing these stem cells for clinical purposes requires careful culture techniques such as excluding animal-derived supplements. Human platelet lysate (hPL) has emerged as a safer alternative to fetal bovine serum (FBS) for cell culture. In our study, we assessed the impact of hPL as a growth factor supplement for culture medium, also conducting a characterization of SHED cultured in hPL-supplemented medium (hPL-SHED). The results showed that hPL has effects in enhancing cell proliferation and migration and increasing cell survivability in oxidative stress conditions induced by H 2 O 2 . The morphology of hPL-SHED exhibited reduced size and elongation, with a differentiation capacity comparable to or even exceeding that of SHED cultured in a medium supplemented with fetal bovine serum (FBS-SHED). Moreover, no evidence of chromosome abnormalities or tumor formation was detected. In conclusion, hPL-SHED emerges as a promising candidate for cell therapy, exhibiting considerable potential for clinical investigation.
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معلومات مُعتمدة: 2022R1F1A1063017 National Research Foundation of Korea; S3282274 Small and Medium Business Administration
فهرسة مساهمة: Keywords: DPSC; FBS alternative; SHED; cell therapy; clinical application of stem cells; hPL; human dental pulp stem cells; human platelet lysate; stem cells derived from human exfoliated deciduous teeth
المشرفين على المادة: 0 (Culture Media)
0 (Cell Extracts)
BBX060AN9V (Hydrogen Peroxide)
تواريخ الأحداث: Date Created: 20240524 Date Completed: 20240524 Latest Revision: 20240526
رمز التحديث: 20240526
مُعرف محوري في PubMed: PMC11120611
DOI: 10.3390/cells13100847
PMID: 38786069
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells13100847