دورية أكاديمية
Japanese encephalitis virus hijacks ER-associated degradation regulators for its replication.
| العنوان: | Japanese encephalitis virus hijacks ER-associated degradation regulators for its replication. |
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| المؤلفون: | Sarkar R; Virology Research Group, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, 121001, India.; Centre for Tuberculosis Research, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, 121001, India.; Present address: Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA., Chhabra S; Virology Research Group, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, 121001, India., Tanwar M; Virology Research Group, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, 121001, India., Agarwal N; Centre for Tuberculosis Research, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, 121001, India., Kalia M; Virology Research Group, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, 121001, India. |
| المصدر: | The Journal of general virology [J Gen Virol] 2024 May; Vol. 105 (5). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print Cited Medium: Internet ISSN: 1465-2099 (Electronic) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology. |
| مواضيع طبية MeSH: | Virus Replication* , Encephalitis Virus, Japanese*/physiology , Encephalitis Virus, Japanese*/genetics , Endoplasmic Reticulum-Associated Degradation* , Ubiquitin-Protein Ligases*/metabolism , Ubiquitin-Protein Ligases*/genetics , Membrane Proteins*/metabolism , Membrane Proteins*/genetics, Humans ; HeLa Cells ; Host-Pathogen Interactions ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum/virology ; Proteins/metabolism ; Proteins/genetics ; Antigens, Differentiation |
| مستخلص: | Flaviviruses target their replication on membranous structures derived from the ER, where both viral and host proteins play crucial structural and functional roles. Here, we have characterized the involvement of the ER-associated degradation (ERAD) pathway core E3 ligase complex (SEL1L-HRD1) regulator proteins in the replication of Japanese encephalitis virus (JEV). Through high-resolution immunofluorescence imaging of JEV-infected HeLa cells, we observe that the virus replication complexes marked by NS1 strongly colocalize with the ERAD adapter SEL1L, lectin OS9, ER-membrane shuttle factor HERPUD1, E3 ubiquitin ligase HRD1 and rhomboid superfamily member DERLIN1. NS5 positive structures also show strong overlap with SEL1L. While these effectors show significant transcriptional upregulation, their protein levels remain largely stable in infected cells. siRNA mediated depletion of OS9, SEL1L, HERPUD1 and HRD1 significantly inhibit viral RNA replication and titres, with SEL1L depletion showing the maximum attenuation of replication. By performing protein translation arrest experiments, we show that SEL1L, and OS9 are stabilised upon JEV infection. Overall results from this study suggest that these ERAD effector proteins are crucial host-factors for JEV replication. |
| فهرسة مساهمة: | Keywords: DERLIN1; ERAD; HERPUD1; HRD1; OS9; SEL1L; flavivirus; retrotranslocon; ubiquitin proteasome |
| المشرفين على المادة: | 0 (SEL1L protein, human) EC 2.3.2.27 (Ubiquitin-Protein Ligases) EC 2.3.2.27 (SYVN1 protein, human) 0 (Membrane Proteins) 0 (HERPUD1 protein, human) 0 (DERL1 protein, human) 0 (leu-13 antigen) 0 (Proteins) 0 (Antigens, Differentiation) |
| تواريخ الأحداث: | Date Created: 20240524 Date Completed: 20240524 Latest Revision: 20240524 |
| رمز التحديث: | 20240524 |
| DOI: | 10.1099/jgv.0.001995 |
| PMID: | 38787366 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2099 |
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| DOI: | 10.1099/jgv.0.001995 |