دورية أكاديمية
The Effect of Whey Protein Isolate Hydrolysate on Digestive Properties of Phytosterol.
| العنوان: | The Effect of Whey Protein Isolate Hydrolysate on Digestive Properties of Phytosterol. |
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| المؤلفون: | Zhao T; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China., Sun H; Yichun Dahaigui Life Science Co., Ltd., Yichun 336000, China., Ji S; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China., Yang B; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China., Wang Z; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China., Liu Y; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China., Chen C; Center for Ultrasound Molecular Imaging and Therapeutics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Lu B; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products of Ministry of Agriculture and Rural Affairs, Key Laboratory for Quality and Safety Risk Assessment of Agro-Products Storage and Preservation of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jun 05; Vol. 72 (22), pp. 12738-12751. Date of Electronic Publication: 2024 May 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Phytosterols*/chemistry , Phytosterols*/metabolism , Whey Proteins*/chemistry , Whey Proteins*/metabolism , Protein Hydrolysates*/chemistry , Protein Hydrolysates*/metabolism , Digestion*, Hydrolysis ; Biological Availability ; Hydrogen Bonding ; Subtilisins/chemistry ; Subtilisins/metabolism ; Humans ; Animals |
| مستخلص: | Phytosterol (PS) is a steroid, and its bioavailability can be enhanced by interacting with protein in the C-24 hydroxyl group. The interaction between sterols and amino acid residues in proteins can be enhanced by enzymatic hydrolysis. Phytosterol and whey insulation hydrolysates (WPH1-4) fabricated by the Alcalase enzyme at different enzymatic hydrolysis times were selected as delivery systems to simulate sterol C-24 hydroxyl group interaction with protein. Increasing hydrolysis time can promote the production of β-Lg, which raises the ratio of β-turn in the secondary structure and promotes the formation of interaction between WPH and PS. The correlation coefficient between hydrogen bonds and encapsulation efficiency (EE) and bioaccessibility is 0.91 and 0.88 ( P < 0.05), respectively, indicating that hydrogen bonds of two components significantly influenced the combination by concealing the hydrophobic amino acids and some residues, which improved PS EE and bioavailability by 3.03 and 2.84 times after PS was combined with the WPI hydrolysate. These findings are expected to enhance the absorption of PS and other macromolecules by protein enzymatic hydrolysis to broaden their applications for food. |
| فهرسة مساهمة: | Keywords: bioaccessibility; hydrolysate; interaction force; phytosterol; secondary structure; whey protein isolate |
| المشرفين على المادة: | 0 (Phytosterols) 0 (Whey Proteins) 0 (Protein Hydrolysates) EC 3.4.21.- (Subtilisins) |
| تواريخ الأحداث: | Date Created: 20240524 Date Completed: 20240605 Latest Revision: 20240605 |
| رمز التحديث: | 20240605 |
| DOI: | 10.1021/acs.jafc.4c01111 |
| PMID: | 38788151 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.4c01111 |