دورية أكاديمية
Fermenter scale production of recombinant beta-mannanase by E. coli BL21 cells under microaerobic environment.
| العنوان: | Fermenter scale production of recombinant beta-mannanase by E. coli BL21 cells under microaerobic environment. |
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| المؤلفون: | Purohit A; Center of Innovative and Applied Bioprocessing (CIAB), Sector-81, Knowledge City, Mohali, 140306, India., Pawar L; Center of Innovative and Applied Bioprocessing (CIAB), Sector-81, Knowledge City, Mohali, 140306, India., Yadav SK; Center of Innovative and Applied Bioprocessing (CIAB), Sector-81, Knowledge City, Mohali, 140306, India; CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, (HP), India. Electronic address: sudesh@ciab.res.in. |
| المصدر: | Carbohydrate research [Carbohydr Res] 2024 Jul; Vol. 541, pp. 109150. Date of Electronic Publication: 2024 May 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0043535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-426X (Electronic) Linking ISSN: 00086215 NLM ISO Abbreviation: Carbohydr Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam. |
| مواضيع طبية MeSH: | beta-Mannosidase*/metabolism , beta-Mannosidase*/genetics , beta-Mannosidase*/biosynthesis , beta-Mannosidase*/chemistry , Escherichia coli*/metabolism , Escherichia coli*/genetics , Recombinant Proteins*/biosynthesis , Recombinant Proteins*/metabolism , Recombinant Proteins*/genetics , Fermentation*, Mannans/metabolism ; Mannans/chemistry ; Mannans/biosynthesis ; Bioreactors ; Hydrogen-Ion Concentration ; Aerobiosis ; Galactans/metabolism ; Galactans/biosynthesis ; Galactans/chemistry ; Culture Media/chemistry ; Culture Media/metabolism ; Plant Gums/chemistry ; Plant Gums/metabolism ; Actinobacteria/enzymology ; Actinobacteria/metabolism ; Actinobacteria/genetics ; Hydrolysis |
| مستخلص: | Aim of the study was to optimize and produce beta-mannanase at fermenter scale by using cheaper minimal media. Increased production of beta-mannanase from Microbacterium camelliasinensis CIAB417 was achieved by heterologous expression in E. coli BL21 (DE3). The scale-up production of beta-mannanase was optimized from shake flask to 5-L fermenter. The cost-effective minimal media (M9+e) without any vitamins was found to be most effective and optimized for culturing the cells. The same media displayed no significant fluctuation in the pH while culturing the cells for the production of beta-mannanase both at shake flask and fermenter level. Additionally, E. coli cells were able to produce similar amount of dry cell weight and recombinant beta-mannanase both in the presence of micro and macro-oxygen environment. The optimized media was demonstrated to show no significant drop in pH throughout the recombinant protein production process. In one litre medium, 2.0314 g dry weight of E. coli cells yielded 1.8 g of purified recombinant beta-mannanase. The purified enzyme was lyophilized and demonstrated to hydrolyse locust bean gum to release mannooligosaccharides. Competing Interests: Declaration of competing interest Authors declare that there is no conflict of interest. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Beta-mannanase; Fermenter; Locust bean gum; Mannooligosachharides; Minimal medium |
| المشرفين على المادة: | EC 3.2.1.25 (beta-Mannosidase) 0 (Recombinant Proteins) 0 (Mannans) V4716MY704 (locust bean gum) 0 (Galactans) 0 (Culture Media) 0 (Plant Gums) |
| تواريخ الأحداث: | Date Created: 20240524 Date Completed: 20240613 Latest Revision: 20240627 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1016/j.carres.2024.109150 |
| PMID: | 38788560 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-426X |
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| DOI: | 10.1016/j.carres.2024.109150 |