دورية أكاديمية
Salvaging donated kidneys from prolonged warm ischemia during ex vivo hypothermic oxygenated perfusion.
| العنوان: | Salvaging donated kidneys from prolonged warm ischemia during ex vivo hypothermic oxygenated perfusion. |
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| المؤلفون: | Zhang P; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Sun C; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Mo S; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Hu C; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Ning Y; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Liang H; Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China., Liu Z; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Fan X; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China., Wang Y; Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, P.R. China; Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Wuhan, Hubei, P.R. China. Electronic address: yanfengwang@whu.edu.cn. |
| المصدر: | Kidney international [Kidney Int] 2024 Aug; Vol. 106 (2), pp. 273-290. Date of Electronic Publication: 2024 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0323470 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1755 (Electronic) Linking ISSN: 00852538 NLM ISO Abbreviation: Kidney Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : New York : Elsevier Original Publication: New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Warm Ischemia*/adverse effects , Kidney*/blood supply , Kidney*/pathology , Kidney*/drug effects , Perfusion*/methods , Kidney Transplantation*/adverse effects , Kidney Transplantation*/methods , Organ Preservation*/methods , Organometallic Compounds*/administration & dosage , Organometallic Compounds*/pharmacology, Animals ; Humans ; Male ; Reperfusion Injury/prevention & control ; Reperfusion Injury/etiology ; Reperfusion Injury/pathology ; Rats ; Oxygen/metabolism ; Oxidative Stress/drug effects ; Apoptosis/drug effects ; Microcirculation/drug effects ; Time Factors ; Human Umbilical Vein Endothelial Cells/drug effects |
| مستخلص: | Prolonged warm ischemic is the main cause discarding donated organs after cardiac death. Here, we identified that prolonged warm ischemic time induced disseminated intravascular coagulation and severe capillary vasospasm after cardiac death of rat kidneys. Additionally, we found a significant accumulation of fibrinogen in a hypoxic cell culture of human umbilical vein epithelial cells and in isolated kidneys exposed to prolonged warm ischemic following flushing out of blood. However, pre-flushing the kidney with snake venom plasmin in a 90-minute warm ischemic model maximized removal of micro thrombi and facilitated the delivery of oxygen and therapeutic agents. Application of carbon monoxide-releasing CORM-401 during ex vivo hypothermic oxygenated perfusion achieved multipath protective effects in prolonged warm ischemic kidneys. This led to significant improvements in perfusion parameters, restoration of the microcirculation, amelioration of mitochondrial injury, oxidative stress, and apoptosis. This benefit resulted in significantly prolonged warm ischemic kidney recipient survival rates of 70%, compared with none in those receiving ex vivo hypothermic oxygenated perfusion alone. Significantly, ex vivo hypothermic oxygenated perfusion combined with cytoprotective carbon monoxide releasing CORM-401 treatment meaningfully protected the donated kidney after cardiac death from ischemia-reperfusion injury by reducing inflammation, oxidative stress, apoptosis, and pathological damage. Thus, our study suggests a new combination treatment strategy to potentially expand the donor pool by increasing use of organs after cardiac death and salvaging prolonged warm ischemic kidneys. (Copyright © 2024 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: carbon monoxide releasing molecule-401; donation after cardiac death; hypothermic oxygenated perfusion; plasmin; warm ischemia kidney |
| المشرفين على المادة: | 0 (Organometallic Compounds) 0 (tricarbonylchloro(glycinato)ruthenium(II)) S88TT14065 (Oxygen) |
| تواريخ الأحداث: | Date Created: 20240524 Date Completed: 20240720 Latest Revision: 20240720 |
| رمز التحديث: | 20240722 |
| DOI: | 10.1016/j.kint.2024.04.018 |
| PMID: | 38789038 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1523-1755 |
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| DOI: | 10.1016/j.kint.2024.04.018 |