دورية أكاديمية
أعرض في EDS

Investigating the Transepithelial Transport and Enzymatic Stability of Lactononadecapeptide (NIPPLTQTPVVVPPFLQPE), a 19-Amino Acid Casein-Derived Peptide in Caco-2 Cells.

التفاصيل البيبلوغرافية
العنوان: Investigating the Transepithelial Transport and Enzymatic Stability of Lactononadecapeptide (NIPPLTQTPVVVPPFLQPE), a 19-Amino Acid Casein-Derived Peptide in Caco-2 Cells.
المؤلفون: Nakatani E; Laboratory of Pharmaceutics, Faculty of Pharmaceutical Sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan., Sasai M; Core Technology Laboratories, Asahi Quality and Innovations, Ltd., 1-1-21 Midori, Moriya, Ibaraki 302-0106, Japan., Miyazaki H; Core Technology Laboratories, Asahi Quality and Innovations, Ltd., 1-1-21 Midori, Moriya, Ibaraki 302-0106, Japan., Tanaka S; Laboratory of Pharmaceutics, Faculty of Pharmaceutical Sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan., Hirota T; Core Technology Laboratories, Asahi Quality and Innovations, Ltd., 1-1-21 Midori, Moriya, Ibaraki 302-0106, Japan., Okura T; Laboratory of Pharmaceutics, Faculty of Pharmaceutical Sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jun 05; Vol. 72 (22), pp. 12719-12724. Date of Electronic Publication: 2024 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Caseins*/metabolism , Caseins*/chemistry , Caseins*/genetics, Humans ; Caco-2 Cells ; Biological Transport ; Peptide Transporter 1/genetics ; Peptide Transporter 1/metabolism ; Intestinal Mucosa/metabolism ; Enzyme Stability ; Peptides/chemistry ; Peptides/metabolism
مستخلص: Lactononadecapeptide (LNDP; NIPPLTQTPVVVPPFLQPE), a casein-derived peptide comprising 19 residues, is known for its capacity to enhance cognitive function. This study aimed to explore the transepithelial transport and stability of LNDP. Results showed that LNDP retained over 90% stability after 2 h of treatment with gastrointestinal enzymes. The stability of LNDP on Caco-2 cell monolayers ranged from 93.4% ± 0.9% to 101.1% ± 1.2% over a period of 15-60 min, with no significant differences at each time point. The permeability of LNDP across an artificial lipid membrane was very low with the effective permeability of 3.6 × 10 -11 cm/s. The Caco-2 assay demonstrated that LNDP could traverse the intestinal epithelium, with an apparent permeability of 1.22 × 10 -6 cm/s. Its transport was significantly inhibited to 67.9% ± 5.0% of the control by Gly-Pro, a competitor of peptide transporter 1 (PEPT1). Furthermore, PEPT1 knockdown using siRNA significantly inhibited LNDP transport by 77.6% ± 1.9% in Caco-2 cell monolayers. The LNDP uptake in PEPT1-expressing HEK293 cells was significantly higher (54.5% ± 14.6%) than that in mock cells. These findings suggest that PEPT1 plays a crucial role in LNDP transport, and LNDP exhibits good resistance to gastrointestinal enzymes.
References: Mol Nutr Food Res. 2008 Dec;52(12):1507-13. (PMID: 18727013)
Am J Physiol Gastrointest Liver Physiol. 2002 Oct;283(4):G996-G1003. (PMID: 12223360)
Nutr Res Rev. 2016 Jun;29(1):91-101. (PMID: 27109024)
Nahrung. 1999 Jun;43(3):154-8. (PMID: 10399347)
Asian J Pharm Sci. 2018 Nov;13(6):555-565. (PMID: 32104429)
J Agric Food Chem. 2023 Aug 30;71(34):12749-12756. (PMID: 37587911)
J Agric Food Chem. 2008 May 28;56(10):3582-6. (PMID: 18442243)
J Pept Sci. 2007 Jul;13(7):468-74. (PMID: 17554807)
J Agric Food Chem. 2014 Apr 9;62(14):3177-3182. (PMID: 24670259)
J Biol Chem. 1998 Feb 13;273(7):3861-4. (PMID: 9461567)
Biochem Biophys Res Commun. 1991 Mar 29;175(3):880-5. (PMID: 1673839)
J Dairy Sci. 1995 Jun;78(6):1253-7. (PMID: 7673515)
Pharm Res. 2005 Sep;22(9):1510-8. (PMID: 16132363)
Curr Protoc Protein Sci. 2001 Nov;Chapter 21:Unit 21.3. (PMID: 18429164)
Biosci Biotechnol Biochem. 2002 Feb;66(2):378-84. (PMID: 11999412)
Gastroenterology. 1989 Mar;96(3):736-49. (PMID: 2914637)
J Agric Food Chem. 2022 Apr 27;70(16):4934-4941. (PMID: 35436096)
Org Biomol Chem. 2003 May 7;1(9):1498-502. (PMID: 12926278)
Nutr Neurosci. 2015 Jul;18(5):232-40. (PMID: 24694020)
Biochim Biophys Acta. 1974 Nov 25;370(1):283-96. (PMID: 4214560)
Biochem J. 1992 Jun 1;284 ( Pt 2):595-601. (PMID: 1318037)
J Agric Food Chem. 2017 Aug 30;65(34):7406-7414. (PMID: 28782363)
Peptides. 2022 Sep;155:170843. (PMID: 35878657)
Molecules. 2019 Aug 05;24(15):. (PMID: 31387268)
J Proteome Res. 2008 Jan;7(1):300-5. (PMID: 18067249)
Int J Food Sci Nutr. 2018 May;69(3):369-376. (PMID: 28819993)
J Chem Inf Model. 2021 Jul 26;61(7):3681-3695. (PMID: 34236179)
Curr Opin Pharmacol. 2013 Dec;13(6):881-7. (PMID: 24007794)
Neurobiol Aging. 2018 Dec;72:23-31. (PMID: 30176402)
Biosci Biotechnol Biochem. 2021 Mar 24;85(4):948-956. (PMID: 33580692)
Biochem Biophys Res Commun. 2018 Sep 5;503(2):1070-1074. (PMID: 29936178)
J Cell Biol. 1986 Jun;102(6):2125-36. (PMID: 3711143)
J Dairy Sci. 2004 Oct;87(10):3183-8. (PMID: 15377596)
Nature. 1994 Apr 7;368(6471):563-6. (PMID: 8139693)
J Dairy Sci. 1996 Dec;79(12):2112-20. (PMID: 9029350)
Pharmaceutics. 2023 Oct 23;15(10):. (PMID: 37896276)
J Biol Chem. 1995 Nov 24;270(47):28425-32. (PMID: 7499348)
J Pept Sci. 2002 Mar;8(3):95-100. (PMID: 11931586)
J Pharm Sci. 2004 Jun;93(6):1440-53. (PMID: 15124203)
Molecules. 2023 Jan 24;28(3):. (PMID: 36770817)
J Agric Food Chem. 2015 Sep 23;63(37):8143-50. (PMID: 26335384)
FASEB J. 2019 Dec;33(12):14095-14102. (PMID: 31652095)
Food Chem. 2016 Jan 1;190:681-688. (PMID: 26213026)
Int J Food Sci Nutr. 2018 Feb;69(1):33-45. (PMID: 28535697)
Mol Nutr Food Res. 2010 Oct;54(10):1428-35. (PMID: 20397193)
فهرسة مساهمة: Keywords: Caco-2 cells; beta-casein; intestinal transport; lactononadecapeptide (LNDP); peptide; peptide transporter 1 (PEPT1)
المشرفين على المادة: 0 (Caseins)
0 (Peptide Transporter 1)
0 (Peptides)
تواريخ الأحداث: Date Created: 20240524 Date Completed: 20240605 Latest Revision: 20240609
رمز التحديث: 20240609
مُعرف محوري في PubMed: PMC11157532
DOI: 10.1021/acs.jafc.4c00974
PMID: 38789103
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.4c00974