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Influence of the Tissue Collection Procedure on the Adipogenic Differentiation of Human Stem Cells: Ischemic versus Well-Vascularized Adipose Tissue.

التفاصيل البيبلوغرافية
العنوان: Influence of the Tissue Collection Procedure on the Adipogenic Differentiation of Human Stem Cells: Ischemic versus Well-Vascularized Adipose Tissue.
المؤلفون: Pal P; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Medina A; Division of Plastic Surgery, Department of Surgery, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Chowdhury S; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Cates CA; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Bollavarapu R; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Person JM; Cancer Institute, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., McIntyre B; Division of Plastic Surgery, Department of Surgery, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Speed JS; Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA., Janorkar AV; Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS 39216, USA.
المصدر: Biomedicines [Biomedicines] 2024 May 01; Vol. 12 (5). Date of Electronic Publication: 2024 May 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101691304 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9059 (Print) Linking ISSN: 22279059 NLM ISO Abbreviation: Biomedicines Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: Clinical and basic science applications using adipose-derived stem cells (ADSCs) are gaining popularity. The current adipose tissue harvesting procedures introduce nonphysiological conditions, which may affect the overall performance of the isolated ADSCs. In this study, we elucidate the differences between ADSCs isolated from adipose tissues harvested within the first 5 min of the initial surgical incision (well-vascularized, nonpremedicated condition) versus those isolated from adipose tissues subjected to medications and deprived of blood supply during elective free flap procedures (ischemic condition). ADSCs isolated from well-vascularized and ischemic tissues positively immunostained for several standard stem cell markers. Interestingly, the percent change in the CD36 expression for ADSCs isolated from ischemic versus well-vascularized tissue was significantly lower in males than females ( p < 0.05). Upon differentiation and maturation to adipocytes, spheroids formed using ADSCs isolated from ischemic adipose tissue had lower triglyceride content compared to those formed using ADSCs isolated from the well-vascularized tissue ( p < 0.05). These results indicate that ADSCs isolated from ischemic tissue either fail to uptake fatty acids or fail to efficiently convert those fatty acids into triglycerides. Therefore, more robust ADSCs suitable to establish in vitro adipose tissue models can be obtained by harvesting well-vascularized and nonpremedicated adipose tissues.
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معلومات مُعتمدة: P20GM121334 United States GM NIGMS NIH HHS; R01EB020006 United States EB NIBIB NIH HHS; P20GM104357 United States GM NIGMS NIH HHS; P30 GM149404 United States GM NIGMS NIH HHS; P20 GM104357 United States GM NIGMS NIH HHS; R01DK124327 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: 3D spheroid; CD36; adipose tissue; flow cytometry; triglyceride
تواريخ الأحداث: Date Created: 20240525 Latest Revision: 20240723
رمز التحديث: 20240723
مُعرف محوري في PubMed: PMC11117639
DOI: 10.3390/biomedicines12050997
PMID: 38790959
قاعدة البيانات: MEDLINE
الوصف
تدمد:2227-9059
DOI:10.3390/biomedicines12050997