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Zebrafish Avatars: Toward Functional Precision Medicine in Low-Grade Serous Ovarian Cancer.

التفاصيل البيبلوغرافية
العنوان: Zebrafish Avatars: Toward Functional Precision Medicine in Low-Grade Serous Ovarian Cancer.
المؤلفون: Fieuws C; Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.; Center for Medical Genetics Ghent, Ghent University Hospital, 9000 Ghent, Belgium.; Cancer Research Institute Ghent, 9000 Ghent, Belgium., Bek JW; Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.; Center for Medical Genetics Ghent, Ghent University Hospital, 9000 Ghent, Belgium.; Cancer Research Institute Ghent, 9000 Ghent, Belgium., Parton B; Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.; Center for Medical Genetics Ghent, Ghent University Hospital, 9000 Ghent, Belgium.; Cancer Research Institute Ghent, 9000 Ghent, Belgium., De Neef E; Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.; Center for Medical Genetics Ghent, Ghent University Hospital, 9000 Ghent, Belgium.; Cancer Research Institute Ghent, 9000 Ghent, Belgium., De Wever O; Cancer Research Institute Ghent, 9000 Ghent, Belgium.; Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, 9000 Ghent, Belgium., Hoorne M; Cancer Research Institute Ghent, 9000 Ghent, Belgium.; Department of Medical Oncology, Ghent University Hospital, 9000 Ghent, Belgium., Estrada MF; Champalimaud Centre of the Unknown, Champalimaud Foundation, 1400-038 Lisbon, Portugal., Van Dorpe J; Cancer Research Institute Ghent, 9000 Ghent, Belgium.; Department of Pathology, Ghent University Hospital, 9000 Ghent, Belgium., Denys H; Cancer Research Institute Ghent, 9000 Ghent, Belgium.; Department of Medical Oncology, Ghent University Hospital, 9000 Ghent, Belgium., Van de Vijver K; Cancer Research Institute Ghent, 9000 Ghent, Belgium.; Department of Pathology, Ghent University Hospital, 9000 Ghent, Belgium., Claes KBM; Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.; Center for Medical Genetics Ghent, Ghent University Hospital, 9000 Ghent, Belgium.; Cancer Research Institute Ghent, 9000 Ghent, Belgium.
المصدر: Cancers [Cancers (Basel)] 2024 May 09; Vol. 16 (10). Date of Electronic Publication: 2024 May 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101526829 Publication Model: Electronic Cited Medium: Print ISSN: 2072-6694 (Print) Linking ISSN: 20726694 NLM ISO Abbreviation: Cancers (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مستخلص: Ovarian cancer (OC) is an umbrella term for cancerous malignancies affecting the ovaries, yet treatment options for all subtypes are predominantly derived from high-grade serous ovarian cancer, the largest subgroup. The concept of "functional precision medicine" involves gaining personalized insights on therapy choice, based on direct exposure of patient tissues to drugs. This especially holds promise for rare subtypes like low-grade serous ovarian cancer (LGSOC). This study aims to establish an in vivo model for LGSOC using zebrafish embryos, comparing treatment responses previously observed in mouse PDX models, cell lines and 3D tumor models. To address this goal, a well-characterized patient-derived LGSOC cell line with the KRAS mutation c.35 G>T (p.(Gly12Val)) was used. Fluorescently labeled tumor cells were injected into the perivitelline space of 2 days' post-fertilization zebrafish embryos. At 1 day post-injection, xenografts were assessed for tumor size, followed by random allocation into treatment groups with trametinib, luminespib and trametinib + luminespib. Subsequently, xenografts were euthanized and analyzed for apoptosis and proliferation by confocal microscopy. Tumor cells formed compact tumor masses ( n = 84) in vivo, with clear Ki67 staining, indicating proliferation. Zebrafish xenografts exhibited sensitivity to trametinib and luminespib, individually or combined, within a two-week period, establishing them as a rapid and complementary tool to existing in vitro and in vivo models for evaluating targeted therapies in LGSOC.
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معلومات مُعتمدة: 1S73622N Research Foundation - Flanders
فهرسة مساهمة: Keywords: functional precision medicine; low-grade serous; ovarian cancer; zAvatars; zebrafish xenografts
تواريخ الأحداث: Date Created: 20240525 Latest Revision: 20240527
رمز التحديث: 20240527
مُعرف محوري في PubMed: PMC11120355
DOI: 10.3390/cancers16101812
PMID: 38791891
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6694
DOI:10.3390/cancers16101812