دورية أكاديمية
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Engineering the Signal Resolution of a Paper-Based Cell-Free Glutamine Biosensor with Genetic Engineering, Metabolic Engineering, and Process Optimization.

التفاصيل البيبلوغرافية
العنوان: Engineering the Signal Resolution of a Paper-Based Cell-Free Glutamine Biosensor with Genetic Engineering, Metabolic Engineering, and Process Optimization.
المؤلفون: Free TJ; Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA., Talley JP; Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA., Hyer CD; Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA., Miller CJ; Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA., Griffitts JS; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA., Bundy BC; Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA.
المصدر: Sensors (Basel, Switzerland) [Sensors (Basel)] 2024 May 12; Vol. 24 (10). Date of Electronic Publication: 2024 May 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101204366 Publication Model: Electronic Cited Medium: Internet ISSN: 1424-8220 (Electronic) Linking ISSN: 14248220 NLM ISO Abbreviation: Sensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c2000-
مواضيع طبية MeSH: Biosensing Techniques*/methods , Glutamine*/metabolism , Metabolic Engineering*/methods, Humans ; Genetic Engineering/methods ; Paper ; Colorimetry/methods ; Cell-Free System
مستخلص: Specialized cancer treatments have the potential to exploit glutamine dependence to increase patient survival rates. Glutamine diagnostics capable of tracking a patient's response to treatment would enable a personalized treatment dosage to optimize the tradeoff between treatment success and dangerous side effects. Current clinical glutamine testing requires sophisticated and expensive lab-based tests, which are not broadly available on a frequent, individualized basis. To address the need for a low-cost, portable glutamine diagnostic, this work engineers a cell-free glutamine biosensor to overcome assay background and signal-to-noise limitations evident in previously reported studies. The findings from this work culminate in the development of a shelf-stable, paper-based, colorimetric glutamine test with a high signal strength and a high signal-to-background ratio for dramatically improved signal resolution. While the engineered glutamine test is important progress towards improving the management of cancer and other health conditions, this work also expands the assay development field of the promising cell-free biosensing platform, which can facilitate the low-cost detection of a broad variety of target molecules with high clinical value.
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معلومات مُعتمدة: Graduate Research Fellowship The Simmons Center for Cancer Research at Brigham Young University
فهرسة مساهمة: Keywords: biosensor; cancer; cell-free protein synthesis (CFPS); glutamine
المشرفين على المادة: 0RH81L854J (Glutamine)
تواريخ الأحداث: Date Created: 20240525 Date Completed: 20240525 Latest Revision: 20240527
رمز التحديث: 20240527
مُعرف محوري في PubMed: PMC11124800
DOI: 10.3390/s24103073
PMID: 38793927
قاعدة البيانات: MEDLINE
الوصف
تدمد:1424-8220
DOI:10.3390/s24103073