دورية أكاديمية
أعرض في EDS

Glycyrrhizic Acid Nanoparticles Subside the Activity of Methicillin-Resistant Staphylococcus aureus by Suppressing PBP2a.

التفاصيل البيبلوغرافية
العنوان: Glycyrrhizic Acid Nanoparticles Subside the Activity of Methicillin-Resistant Staphylococcus aureus by Suppressing PBP2a.
المؤلفون: Rijo P; CBIOS-Lusófona University's Research Center for Biosciences and Health Technologies, 1749-024 Lisbon, Portugal.; Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisbon, Portugal., Abuamara TMM; Department of Basic Medical Science, Faculty of Dentistry, Al-Ahliyya Amman University, Amman 19111, Jordan.; Department of Histology, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt., Ali Lashin LS; Department of Basic Medical Science, Faculty of Dentistry, Al-Ahliyya Amman University, Amman 19111, Jordan.; Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., Kamar SA; Department of Basic Medical Science, Faculty of Dentistry, Al-Ahliyya Amman University, Amman 19111, Jordan.; Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt., Isca VMS; CBIOS-Lusófona University's Research Center for Biosciences and Health Technologies, 1749-024 Lisbon, Portugal., Mohammed TS; Department of Public Health and Community Medicine, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt., Abdrabo MSM; Department of Public Health and Community Medicine, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt., Amin MA; Department of Basic Medical Science, Faculty of Dentistry, Zarqa University, Zarqa 13110, Jordan.; Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt., Abd El Maksoud AI; College of Biotechnology, Misr University of Science and Technology, Giza 12573, Egypt.; Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat 32897, Egypt., Hassan A; Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat 32897, Egypt.
المصدر: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2024 May 06; Vol. 17 (5). Date of Electronic Publication: 2024 May 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101238453 Publication Model: Electronic Cited Medium: Print ISSN: 1424-8247 (Print) Linking ISSN: 14248247 NLM ISO Abbreviation: Pharmaceuticals (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c2004-
مستخلص: Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) are classified as high-risk infections that can lead to death, particularly among older individuals. Nowadays, plant nanoparticles such as glycyrrhizic acid are recognized as efficient bactericides against a wide range of bacterial strains. Recently, scientists have shown interest in plant extract nanoparticles, derived from natural sources, which can be synthesized into nanomaterials. Interestingly, glycyrrhizic acid is rich in antioxidants as well as antibacterial agents, and it exhibits no adverse effects on normal cells. In this study, glycyrrhizic acid nanoparticles (GA-NPs) were synthesized using the hydrothermal method and characterized through physicochemical techniques such as UV-visible spectrometry, DLS, zeta potential, and TEM. The antimicrobial activity of GA-NPs was investigated through various methods, including MIC assays, anti-biofilm activity assays, ATPase activity assays, and kill-time assays. The expression levels of mecA , mecR1 , blaR1 , and blaZ genes were measured by quantitative RT-qPCR. Additionally, the presence of the penicillin-binding protein 2a (PBP2a) protein of S. aureus and MRSA was evaluated by a Western blot assay. The results emphasized the fabrication of GA nanoparticles in spherical shapes with a diameter in the range of 40-50 nm. The data show that GA nanoparticles exhibit great bactericidal effectiveness against S. aureus and MRSA. The treatment with GA-NPs remarkably reduces the expression levels of the mecA , mecR1 , blaR1 , and blaZ genes. PBP2a expression in MRSA was significantly reduced after treatment with GA-NPs. Overall, this study demonstrates that glycyrrhizic acid nanoparticles have potent antibacterial activity, particularly against MRSA. This research elucidates the inhibition mechanism of glycyrrhizic acid, which involves the suppressing of PBP2a expression. This work emphasizes the importance of utilizing plant nanoparticles as effective antimicrobial agents against a broad spectrum of bacteria.
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معلومات مُعتمدة: DOI: 10.54499/UIDP/04567/2020 Fundação para a Ciência e Tecnologia; and DOI: 10.54499/UIDB/04567/2020 Fundação para a Ciência e Tecnologia
فهرسة مساهمة: Keywords: MRSA; glycyrrhizic acid nanoparticles; mecA; mecR1; penicillin-binding protein 2a
تواريخ الأحداث: Date Created: 20240525 Latest Revision: 20240527
رمز التحديث: 20240527
مُعرف محوري في PubMed: PMC11123903
DOI: 10.3390/ph17050589
PMID: 38794159
قاعدة البيانات: MEDLINE
الوصف
تدمد:1424-8247
DOI:10.3390/ph17050589