دورية أكاديمية
miR-181a/b-5p negatively regulates keratinocytes proliferation by targeting MELK.
| العنوان: | miR-181a/b-5p negatively regulates keratinocytes proliferation by targeting MELK. |
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| المؤلفون: | Niu M; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China.; Key Laboratory of Biochemistry and Molecular Biology, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Li M; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China.; Key Laboratory of Biochemistry and Molecular Biology, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Fan X; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China.; Key Laboratory of Biochemistry and Molecular Biology, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Chen F; Department of Dermatology, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, P. R. China., Wang M; Department of Dermatology, The Second Affiliated Hospital of Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Liu Q; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Liang B; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Gan S; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China.; Key Laboratory of Biochemistry and Molecular Biology, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China., Mo Z; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China. mozhijing@glmc.edu.cn.; Key Laboratory of Biochemistry and Molecular Biology, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China. mozhijing@glmc.edu.cn., Gao J; School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China. jintao_gao@glmc.edu.cn.; Key Laboratory of Biochemistry and Molecular Biology, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China. jintao_gao@glmc.edu.cn. |
| المصدر: | Archives of dermatological research [Arch Dermatol Res] 2024 May 25; Vol. 316 (6), pp. 236. Date of Electronic Publication: 2024 May 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Verlag Country of Publication: Germany NLM ID: 8000462 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-069X (Electronic) Linking ISSN: 03403696 NLM ISO Abbreviation: Arch Dermatol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin : Springer Verlag |
| مواضيع طبية MeSH: | MicroRNAs*/metabolism , MicroRNAs*/genetics , Keratinocytes*/metabolism , Cell Proliferation*/genetics , Psoriasis*/pathology , Psoriasis*/genetics , Psoriasis*/metabolism , Protein Serine-Threonine Kinases*/metabolism , Protein Serine-Threonine Kinases*/genetics , HaCaT Cells*, Humans ; Keratin-16/metabolism ; Keratin-16/genetics ; Down-Regulation ; Cell Survival ; Cell Line |
| مستخلص: | Accumulating evidence indicates that microRNAs (miRNAs) have a vital effect on the pathogenesis of psoriasis. This study is conducted to investigate the potential involvement of miR-181a-5p and miR-181b-5p in the proliferation of HaCaT keratinocytes. Cell viability and proliferation were evaluated respectively in this study using the CCK-8 and the 5-ethynyl-2'-deoxyuridine (EdU) assays. The expression of Maternal Embryonic Leucine Zipper Kinase (MELK) and Keratin 16 (KRT16) mRNA and protein in tissues and cells was assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. The Luciferase reporter system analyzes the connection between miR-181a-5p/miR-181b-5p and MELK. The results showed that miR-181a/b-5p expression was downregulated in the psoriasis lesions and negatively regulated the proliferation of keratinocytes. MELK was directly targeted by miR-181a-5p/miR-181b-5p. In addition, HaCaT keratinocytes proliferation was inhibited by knockdown of MELK while promoted dramatically by MELK overexpression. Notably, miR-181a/b-5p mimics could attenuate the effects of MELK in keratinocytes. In conclusion, our research findings suggested miR-181a-5p and miR-181b-5p negatively regulate keratinocyte proliferation by targeting MELK, providing potential diagnostic biomarkers and therapeutic targets for psoriasis. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| معلومات مُعتمدة: | YCSW2023420 Innovation Project of Guangxi Graduate Education; 32360167 and 31860314 National Natural Science Foundation of China; 2018GXNSFAA281041 and 2016GXNSFBA380146 Natural Science Foundation of Guangxi Zhuang Autonomous Region |
| فهرسة مساهمة: | Keywords: Keratinocytes; MELK; Proliferation; miR-181a-5p; miR-181b-5p |
| المشرفين على المادة: | 0 (MicroRNAs) EC 2.7.11.1 (Protein Serine-Threonine Kinases) 0 (MIrn181 microRNA, human) EC 2.7.1.- (MELK protein, human) 0 (Keratin-16) 0 (KRT16 protein, human) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240525 Latest Revision: 20240626 |
| رمز التحديث: | 20240626 |
| DOI: | 10.1007/s00403-024-03081-2 |
| PMID: | 38795158 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1432-069X |
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| DOI: | 10.1007/s00403-024-03081-2 |